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Expression of the HXT13, HXT15 and HXT17 genes in Saccharomyces cerevisiae and stabilization of the HXT1 gene transcript by sugar-induced osmotic stress.
Curr Genet. 2006 Apr; 49(4):205-17.CG

Abstract

Saccharomyces cerevisiae contains a family of 17 hexose transporter (HXT) genes; only nine have assigned functions, some of which are still poorly defined. Despite extensive efforts to characterize the hexose transporters, the expression of HXT6 and HXT8-17 remains an enigma. In nature, S. cerevisiae finds itself under extreme nutritional conditions including sugars in excess of 40% (w/v), depletion of nutrients and extremes of both temperature and pH. Using HXT promoter-lacZ fusions, we have identified novel conditions under which the HXT17 gene is expressed; HXT17 promoter activity is up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7. We demonstrated that HXT5, HXT13 and, to a lesser extent, HXT15 were all induced in the presence of non-fermentable carbon sources. HXT1 encodes a low-affinity transporter and in short-term osmotic shock experiments, HXT1 promoter activity was reduced when cells were exposed to media containing 40% glucose. However, we found that the HXT1 mRNA transcript was stabilized under conditions of osmotic stress. Furthermore, the stabilization of HXT1 mRNA does not appear to be gene specific because 30 min after transcriptional arrest there is a fourfold more mRNA in osmotically stressed versus non-stressed yeast cells. A large portion of S. cerevisiae mRNA molecules may, therefore, have a decreased rate of turnover during exposure to osmotic stress indicating that post-transcriptional regulation plays an important role in the adaptation of S. cerevisiae to osmotic stress.

Authors+Show Affiliations

Wine Research Centre, The University of British Columbia, Suite 231#2205 East Mall, V6T 1Z4, Vancouver, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16397765

Citation

Greatrix, Bradley W., and Hennie J J. van Vuuren. "Expression of the HXT13, HXT15 and HXT17 Genes in Saccharomyces Cerevisiae and Stabilization of the HXT1 Gene Transcript By Sugar-induced Osmotic Stress." Current Genetics, vol. 49, no. 4, 2006, pp. 205-17.
Greatrix BW, van Vuuren HJ. Expression of the HXT13, HXT15 and HXT17 genes in Saccharomyces cerevisiae and stabilization of the HXT1 gene transcript by sugar-induced osmotic stress. Curr Genet. 2006;49(4):205-17.
Greatrix, B. W., & van Vuuren, H. J. (2006). Expression of the HXT13, HXT15 and HXT17 genes in Saccharomyces cerevisiae and stabilization of the HXT1 gene transcript by sugar-induced osmotic stress. Current Genetics, 49(4), 205-17.
Greatrix BW, van Vuuren HJ. Expression of the HXT13, HXT15 and HXT17 Genes in Saccharomyces Cerevisiae and Stabilization of the HXT1 Gene Transcript By Sugar-induced Osmotic Stress. Curr Genet. 2006;49(4):205-17. PubMed PMID: 16397765.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of the HXT13, HXT15 and HXT17 genes in Saccharomyces cerevisiae and stabilization of the HXT1 gene transcript by sugar-induced osmotic stress. AU - Greatrix,Bradley W, AU - van Vuuren,Hennie J J, Y1 - 2006/01/06/ PY - 2005/09/20/received PY - 2005/11/15/accepted PY - 2005/11/14/revised PY - 2006/1/7/pubmed PY - 2006/5/31/medline PY - 2006/1/7/entrez SP - 205 EP - 17 JF - Current genetics JO - Curr Genet VL - 49 IS - 4 N2 - Saccharomyces cerevisiae contains a family of 17 hexose transporter (HXT) genes; only nine have assigned functions, some of which are still poorly defined. Despite extensive efforts to characterize the hexose transporters, the expression of HXT6 and HXT8-17 remains an enigma. In nature, S. cerevisiae finds itself under extreme nutritional conditions including sugars in excess of 40% (w/v), depletion of nutrients and extremes of both temperature and pH. Using HXT promoter-lacZ fusions, we have identified novel conditions under which the HXT17 gene is expressed; HXT17 promoter activity is up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7. We demonstrated that HXT5, HXT13 and, to a lesser extent, HXT15 were all induced in the presence of non-fermentable carbon sources. HXT1 encodes a low-affinity transporter and in short-term osmotic shock experiments, HXT1 promoter activity was reduced when cells were exposed to media containing 40% glucose. However, we found that the HXT1 mRNA transcript was stabilized under conditions of osmotic stress. Furthermore, the stabilization of HXT1 mRNA does not appear to be gene specific because 30 min after transcriptional arrest there is a fourfold more mRNA in osmotically stressed versus non-stressed yeast cells. A large portion of S. cerevisiae mRNA molecules may, therefore, have a decreased rate of turnover during exposure to osmotic stress indicating that post-transcriptional regulation plays an important role in the adaptation of S. cerevisiae to osmotic stress. SN - 0172-8083 UR - https://www.unboundmedicine.com/medline/citation/16397765/Expression_of_the_HXT13_HXT15_and_HXT17_genes_in_Saccharomyces_cerevisiae_and_stabilization_of_the_HXT1_gene_transcript_by_sugar_induced_osmotic_stress_ DB - PRIME DP - Unbound Medicine ER -