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Hybrid quadrupole/time-of-flight mass spectrometers for analysis of biomolecules.
Methods Enzymol. 2005; 402:49-78.ME

Abstract

The basic principles of quadrupole/time-of-flight (TOF) mass spectrometers are discussed. These instruments can be used for ions produced either by electrospray ionization (ESI) or by matrix-assisted laser desorption ionization (MALDI). In the most common configuration, the functions of collisional cooling, parent ion selection, and collision-induced dissociation are carried out successively in three separate quadrupoles. The ions are then injected orthogonally into a TOF spectrometer, which makes the m/z measurement. Thus, these hybrid instruments benefit from the versatile ability of quadrupoles to carry out various tasks and from the high performance of TOF spectrometers in both simple mass spectrometry (MS) and tandem (MS/MS) modes. Significantly, collisions in the initial quadrupole decouple the instrument almost completely from the ion production process, so the quadrupole/TOF spectrometer is a stable device that is relatively insensitive to variations in the ion source.

Authors+Show Affiliations

Department of Physics and Astronomy, University of Manitoba, Winnipeg, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

16401506

Citation

Ens, Werner, and Kenneth G. Standing. "Hybrid Quadrupole/time-of-flight Mass Spectrometers for Analysis of Biomolecules." Methods in Enzymology, vol. 402, 2005, pp. 49-78.
Ens W, Standing KG. Hybrid quadrupole/time-of-flight mass spectrometers for analysis of biomolecules. Methods Enzymol. 2005;402:49-78.
Ens, W., & Standing, K. G. (2005). Hybrid quadrupole/time-of-flight mass spectrometers for analysis of biomolecules. Methods in Enzymology, 402, 49-78.
Ens W, Standing KG. Hybrid Quadrupole/time-of-flight Mass Spectrometers for Analysis of Biomolecules. Methods Enzymol. 2005;402:49-78. PubMed PMID: 16401506.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hybrid quadrupole/time-of-flight mass spectrometers for analysis of biomolecules. AU - Ens,Werner, AU - Standing,Kenneth G, PY - 2006/1/13/pubmed PY - 2006/3/1/medline PY - 2006/1/13/entrez SP - 49 EP - 78 JF - Methods in enzymology JO - Methods Enzymol VL - 402 N2 - The basic principles of quadrupole/time-of-flight (TOF) mass spectrometers are discussed. These instruments can be used for ions produced either by electrospray ionization (ESI) or by matrix-assisted laser desorption ionization (MALDI). In the most common configuration, the functions of collisional cooling, parent ion selection, and collision-induced dissociation are carried out successively in three separate quadrupoles. The ions are then injected orthogonally into a TOF spectrometer, which makes the m/z measurement. Thus, these hybrid instruments benefit from the versatile ability of quadrupoles to carry out various tasks and from the high performance of TOF spectrometers in both simple mass spectrometry (MS) and tandem (MS/MS) modes. Significantly, collisions in the initial quadrupole decouple the instrument almost completely from the ion production process, so the quadrupole/TOF spectrometer is a stable device that is relatively insensitive to variations in the ion source. SN - 0076-6879 UR - https://www.unboundmedicine.com/medline/citation/16401506/Hybrid_quadrupole/time_of_flight_mass_spectrometers_for_analysis_of_biomolecules_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0076-6879(05)02002-1 DB - PRIME DP - Unbound Medicine ER -