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Tandem time-of-flight mass spectrometry.
Methods Enzymol. 2005; 402:79-108.ME

Abstract

A new tandem time-of-flight (TOF-TOF) instrument has been developed by modifying a standard matrix-assisted laser desorption ionization (MALDI)-TOF instrument to make high-performance, high-energy collision-induced dissociation (CID) MALDI tandem mass spectrometry (MS) a practical reality. To optimize fragment spectra quality, the selected precursor ion is decelerated before entering a floating collision cell and the potential difference between the source and the collision cell defines the collision energy of the ions. Standard operating conditions for tandem MS use a 1-kV collision energy with single-collision conditions and increased laser power for ion formation. Hence, both high- and low-energy fragments are observed in MALDI TOF-TOF spectra. On standard peptides, sensitivities down to 1 fmol are demonstrated. On a mixture of two solution tryptic digests at the 25-fmol level, 23 spectra were sufficient to result in proper database identification.

Authors+Show Affiliations

Applied Biosystems, Framingham, Massachusetts, USA.No affiliation info available

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

16401507

Citation

Vestal, Marvin L., and Jennifer M. Campbell. "Tandem Time-of-flight Mass Spectrometry." Methods in Enzymology, vol. 402, 2005, pp. 79-108.
Vestal ML, Campbell JM. Tandem time-of-flight mass spectrometry. Methods Enzymol. 2005;402:79-108.
Vestal, M. L., & Campbell, J. M. (2005). Tandem time-of-flight mass spectrometry. Methods in Enzymology, 402, 79-108.
Vestal ML, Campbell JM. Tandem Time-of-flight Mass Spectrometry. Methods Enzymol. 2005;402:79-108. PubMed PMID: 16401507.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tandem time-of-flight mass spectrometry. AU - Vestal,Marvin L, AU - Campbell,Jennifer M, PY - 2006/1/13/pubmed PY - 2006/3/1/medline PY - 2006/1/13/entrez SP - 79 EP - 108 JF - Methods in enzymology JO - Methods Enzymol VL - 402 N2 - A new tandem time-of-flight (TOF-TOF) instrument has been developed by modifying a standard matrix-assisted laser desorption ionization (MALDI)-TOF instrument to make high-performance, high-energy collision-induced dissociation (CID) MALDI tandem mass spectrometry (MS) a practical reality. To optimize fragment spectra quality, the selected precursor ion is decelerated before entering a floating collision cell and the potential difference between the source and the collision cell defines the collision energy of the ions. Standard operating conditions for tandem MS use a 1-kV collision energy with single-collision conditions and increased laser power for ion formation. Hence, both high- and low-energy fragments are observed in MALDI TOF-TOF spectra. On standard peptides, sensitivities down to 1 fmol are demonstrated. On a mixture of two solution tryptic digests at the 25-fmol level, 23 spectra were sufficient to result in proper database identification. SN - 0076-6879 UR - https://www.unboundmedicine.com/medline/citation/16401507/Tandem_time_of_flight_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0076-6879(05)02003-3 DB - PRIME DP - Unbound Medicine ER -