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Evaluation of the E test for the assessment of synergy of antibiotic combinations against multiresistant Pseudomonas aeruginosa isolates from cystic fibrosis patients.
Eur J Clin Microbiol Infect Dis. 2006 Jan; 25(1):25-30.EJ

Abstract

The determination of synergistic effects of antimicrobial drug combinations can lead to improved therapeutic options in the antibiotic treatment of cystic fibrosis patients who are chronically infected with multiresistant Pseudomonas aeruginosa isolates. The aim of this study was to evaluate the performance of the E test versus the standard agar dilution checkerboard susceptibility test in the assessment of synergy and, in addition, to determine the activity of two antimicrobial combinations against 163 multiresistant P. aeruginosa isolates from cystic fibrosis patients. The agreement between the checkerboard method and the E test was excellent (>90%) for nonmucoid as well as mucoid isolates from cystic fibrosis patients. The rate of synergy was higher for the antibiotic combination of ceftazidime and tobramycin (28.8% of the cystic fibrosis strains) than for the combination of meropenem and tobramycin (19.0%). However, the probability of synergy for the second antibiotic combination increased significantly when the synergy of the first antibiotic combination had already been demonstrated (Fischer's exact test, p=0.049). The results show that the E test is a valuable and practical method for routine microbiological diagnostics and can aid in the selection of improved antibiotic options in the treatment of cystic fibrosis patients chronically infected with P. aeruginosa.

Authors+Show Affiliations

Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hanover, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16402226

Citation

Balke, B, et al. "Evaluation of the E Test for the Assessment of Synergy of Antibiotic Combinations Against Multiresistant Pseudomonas Aeruginosa Isolates From Cystic Fibrosis Patients." European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, vol. 25, no. 1, 2006, pp. 25-30.
Balke B, Hogardt M, Schmoldt S, et al. Evaluation of the E test for the assessment of synergy of antibiotic combinations against multiresistant Pseudomonas aeruginosa isolates from cystic fibrosis patients. Eur J Clin Microbiol Infect Dis. 2006;25(1):25-30.
Balke, B., Hogardt, M., Schmoldt, S., Hoy, L., Weissbrodt, H., & Häussler, S. (2006). Evaluation of the E test for the assessment of synergy of antibiotic combinations against multiresistant Pseudomonas aeruginosa isolates from cystic fibrosis patients. European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, 25(1), 25-30.
Balke B, et al. Evaluation of the E Test for the Assessment of Synergy of Antibiotic Combinations Against Multiresistant Pseudomonas Aeruginosa Isolates From Cystic Fibrosis Patients. Eur J Clin Microbiol Infect Dis. 2006;25(1):25-30. PubMed PMID: 16402226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the E test for the assessment of synergy of antibiotic combinations against multiresistant Pseudomonas aeruginosa isolates from cystic fibrosis patients. AU - Balke,B, AU - Hogardt,M, AU - Schmoldt,S, AU - Hoy,L, AU - Weissbrodt,H, AU - Häussler,S, PY - 2006/1/13/pubmed PY - 2007/4/6/medline PY - 2006/1/13/entrez SP - 25 EP - 30 JF - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology JO - Eur J Clin Microbiol Infect Dis VL - 25 IS - 1 N2 - The determination of synergistic effects of antimicrobial drug combinations can lead to improved therapeutic options in the antibiotic treatment of cystic fibrosis patients who are chronically infected with multiresistant Pseudomonas aeruginosa isolates. The aim of this study was to evaluate the performance of the E test versus the standard agar dilution checkerboard susceptibility test in the assessment of synergy and, in addition, to determine the activity of two antimicrobial combinations against 163 multiresistant P. aeruginosa isolates from cystic fibrosis patients. The agreement between the checkerboard method and the E test was excellent (>90%) for nonmucoid as well as mucoid isolates from cystic fibrosis patients. The rate of synergy was higher for the antibiotic combination of ceftazidime and tobramycin (28.8% of the cystic fibrosis strains) than for the combination of meropenem and tobramycin (19.0%). However, the probability of synergy for the second antibiotic combination increased significantly when the synergy of the first antibiotic combination had already been demonstrated (Fischer's exact test, p=0.049). The results show that the E test is a valuable and practical method for routine microbiological diagnostics and can aid in the selection of improved antibiotic options in the treatment of cystic fibrosis patients chronically infected with P. aeruginosa. SN - 0934-9723 UR - https://www.unboundmedicine.com/medline/citation/16402226/Evaluation_of_the_E_test_for_the_assessment_of_synergy_of_antibiotic_combinations_against_multiresistant_Pseudomonas_aeruginosa_isolates_from_cystic_fibrosis_patients_ L2 - https://dx.doi.org/10.1007/s10096-005-0076-9 DB - PRIME DP - Unbound Medicine ER -