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Separation and determination of strychnine and brucine in Strychnos nux-vomica L. and its preparation by nonaqueous capillary electrophoresis.
J Pharm Biomed Anal. 2006 May 03; 41(2):400-7.JP

Abstract

An easy, rapid method for simultaneous determination of strychnine and brucine in Strychnos nux-vomica L. and its preparation was developed by nonaqueous capillary electrophoresis (NACE) without pretreatment for the first time. Optimum separation was achieved with a fused-silica capillary column (50 cmx75 microm i.d.) and a running buffer containing 30 mM ammonium acetate, 1.0% acetic acid and 15% acetonitrile (ACN) in methanol medium. The applied voltage was 30.0 kV. The analytes were detected by UV at 214 nm. The effects of concentration of ammonium acetate, acetic acid and organic modifier on electrophoretic behavior of the analytes were studied. The established method with sophoridine as internal standard was linear in the range of 5-1000 mg/mL for both strychnine and brucine. The extracts of Strychnos nux-vomica and its preparation could be directly injected for determination with recoveries ranging from 94.5 to 104%.

Authors+Show Affiliations

Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16406713

Citation

Li, Yuqin, et al. "Separation and Determination of Strychnine and Brucine in Strychnos Nux-vomica L. and Its Preparation By Nonaqueous Capillary Electrophoresis." Journal of Pharmaceutical and Biomedical Analysis, vol. 41, no. 2, 2006, pp. 400-7.
Li Y, He X, Qi S, et al. Separation and determination of strychnine and brucine in Strychnos nux-vomica L. and its preparation by nonaqueous capillary electrophoresis. J Pharm Biomed Anal. 2006;41(2):400-7.
Li, Y., He, X., Qi, S., Gao, W., Chen, X., & Hu, Z. (2006). Separation and determination of strychnine and brucine in Strychnos nux-vomica L. and its preparation by nonaqueous capillary electrophoresis. Journal of Pharmaceutical and Biomedical Analysis, 41(2), 400-7.
Li Y, et al. Separation and Determination of Strychnine and Brucine in Strychnos Nux-vomica L. and Its Preparation By Nonaqueous Capillary Electrophoresis. J Pharm Biomed Anal. 2006 May 3;41(2):400-7. PubMed PMID: 16406713.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and determination of strychnine and brucine in Strychnos nux-vomica L. and its preparation by nonaqueous capillary electrophoresis. AU - Li,Yuqin, AU - He,Xiaojun, AU - Qi,Shengda, AU - Gao,Wenhua, AU - Chen,Xingguo, AU - Hu,Zhide, Y1 - 2006/01/09/ PY - 2005/06/01/received PY - 2005/11/23/revised PY - 2005/11/25/accepted PY - 2006/1/13/pubmed PY - 2007/8/2/medline PY - 2006/1/13/entrez SP - 400 EP - 7 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 41 IS - 2 N2 - An easy, rapid method for simultaneous determination of strychnine and brucine in Strychnos nux-vomica L. and its preparation was developed by nonaqueous capillary electrophoresis (NACE) without pretreatment for the first time. Optimum separation was achieved with a fused-silica capillary column (50 cmx75 microm i.d.) and a running buffer containing 30 mM ammonium acetate, 1.0% acetic acid and 15% acetonitrile (ACN) in methanol medium. The applied voltage was 30.0 kV. The analytes were detected by UV at 214 nm. The effects of concentration of ammonium acetate, acetic acid and organic modifier on electrophoretic behavior of the analytes were studied. The established method with sophoridine as internal standard was linear in the range of 5-1000 mg/mL for both strychnine and brucine. The extracts of Strychnos nux-vomica and its preparation could be directly injected for determination with recoveries ranging from 94.5 to 104%. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/16406713/Separation_and_determination_of_strychnine_and_brucine_in_Strychnos_nux_vomica_L__and_its_preparation_by_nonaqueous_capillary_electrophoresis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(05)00748-X DB - PRIME DP - Unbound Medicine ER -