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Soy moderately improves microstructural properties without affecting bone mass in an ovariectomized rat model of osteoporosis.

Abstract

Soy protein is reported to prevent bone loss in both women and rat models of osteoporosis. However, the role of soy isoflavones on the trabecular microarchitectural properties needs to be explored. In the present study, we examined whether soy protein with graded doses of isoflavones reverses loss of bone mineral density (BMD), bone mineral content (BMC), and trabecular microstructure in an ovariectomized (Ovx) osteopenic rat model. Seventy-eight 9-m old female Sprague-Dawley rats were either sham-operated (Sham; 1 group) or Ovx (5 groups) and fed a semi-purified casein-based diet. After 90 days, the occurrence of bone loss was confirmed using dual energy X-ray absorptiometry. Thereafter, rats were assigned to the following treatments: Sham, Ovx (control), Ovx + 17beta-estradiol (E(2); 10 microg/kg body wt. twice per week), Ovx + soy protein depleted of isoflavones (Soy-; 0.06 mg isoflavones/g protein), Ovx + soy protein with normal isoflavone content (Soy; 3.55 mg isoflavones/g protein), and Ovx + isoflavone-enriched soy protein (Soy+; 7.10 mg isoflavones/g protein). After 125 days of treatment, rats were euthanized, and tibia and lumbar bones were collected for the assessment of BMD, BMC, and trabecular microarchitectural properties using X-ray microcomputed tomography. None of the treatments had an effect on BMD or microarchitectural properties of the lumbar vertebra. However, Soy treatment significantly increased tibial BMC and BMD by 10% and 4.5% compared with Ovx control, but the increase in BMD was not enough to reach the BMD levels of the Sham control group. The Soy+ diet positively affected the tibial architectural properties including trabecular thickness, separation, and number. In summary, our findings suggest that soy protein does not restore bone loss in osteopenic rats; however, higher doses of isoflavones may be required to reverse the loss of tibial microstructural properties.

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  • Authors+Show Affiliations

    ,

    Department of Nutritional Sciences, Oklahoma State University, 301 Human Environmental Sciences, Stillwater, OK 74078-6141, USA.

    , , , , ,

    Source

    Bone 38:5 2006 May pg 686-93

    MeSH

    Absorptiometry, Photon
    Animals
    Body Weight
    Bone Density
    Disease Models, Animal
    Female
    Isoflavones
    Lumbar Vertebrae
    Organ Size
    Osteoporosis
    Ovariectomy
    Placebos
    Rats
    Rats, Sprague-Dawley
    Soybean Proteins
    Soybeans
    Tibia
    Tomography, X-Ray Computed

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    16406762

    Citation

    Devareddy, Latha, et al. "Soy Moderately Improves Microstructural Properties Without Affecting Bone Mass in an Ovariectomized Rat Model of Osteoporosis." Bone, vol. 38, no. 5, 2006, pp. 686-93.
    Devareddy L, Khalil DA, Smith BJ, et al. Soy moderately improves microstructural properties without affecting bone mass in an ovariectomized rat model of osteoporosis. Bone. 2006;38(5):686-93.
    Devareddy, L., Khalil, D. A., Smith, B. J., Lucas, E. A., Soung, d. o. . Y., Marlow, D. D., & Arjmandi, B. H. (2006). Soy moderately improves microstructural properties without affecting bone mass in an ovariectomized rat model of osteoporosis. Bone, 38(5), pp. 686-93.
    Devareddy L, et al. Soy Moderately Improves Microstructural Properties Without Affecting Bone Mass in an Ovariectomized Rat Model of Osteoporosis. Bone. 2006;38(5):686-93. PubMed PMID: 16406762.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Soy moderately improves microstructural properties without affecting bone mass in an ovariectomized rat model of osteoporosis. AU - Devareddy,Latha, AU - Khalil,Dania A, AU - Smith,Brenda J, AU - Lucas,Edralin A, AU - Soung,Do Y, AU - Marlow,Denver D, AU - Arjmandi,Bahram H, Y1 - 2006/01/10/ PY - 2004/07/29/received PY - 2005/09/19/revised PY - 2005/10/13/accepted PY - 2006/1/13/pubmed PY - 2006/7/29/medline PY - 2006/1/13/entrez SP - 686 EP - 93 JF - Bone JO - Bone VL - 38 IS - 5 N2 - Soy protein is reported to prevent bone loss in both women and rat models of osteoporosis. However, the role of soy isoflavones on the trabecular microarchitectural properties needs to be explored. In the present study, we examined whether soy protein with graded doses of isoflavones reverses loss of bone mineral density (BMD), bone mineral content (BMC), and trabecular microstructure in an ovariectomized (Ovx) osteopenic rat model. Seventy-eight 9-m old female Sprague-Dawley rats were either sham-operated (Sham; 1 group) or Ovx (5 groups) and fed a semi-purified casein-based diet. After 90 days, the occurrence of bone loss was confirmed using dual energy X-ray absorptiometry. Thereafter, rats were assigned to the following treatments: Sham, Ovx (control), Ovx + 17beta-estradiol (E(2); 10 microg/kg body wt. twice per week), Ovx + soy protein depleted of isoflavones (Soy-; 0.06 mg isoflavones/g protein), Ovx + soy protein with normal isoflavone content (Soy; 3.55 mg isoflavones/g protein), and Ovx + isoflavone-enriched soy protein (Soy+; 7.10 mg isoflavones/g protein). After 125 days of treatment, rats were euthanized, and tibia and lumbar bones were collected for the assessment of BMD, BMC, and trabecular microarchitectural properties using X-ray microcomputed tomography. None of the treatments had an effect on BMD or microarchitectural properties of the lumbar vertebra. However, Soy treatment significantly increased tibial BMC and BMD by 10% and 4.5% compared with Ovx control, but the increase in BMD was not enough to reach the BMD levels of the Sham control group. The Soy+ diet positively affected the tibial architectural properties including trabecular thickness, separation, and number. In summary, our findings suggest that soy protein does not restore bone loss in osteopenic rats; however, higher doses of isoflavones may be required to reverse the loss of tibial microstructural properties. SN - 8756-3282 UR - https://www.unboundmedicine.com/medline/citation/16406762/Soy_moderately_improves_microstructural_properties_without_affecting_bone_mass_in_an_ovariectomized_rat_model_of_osteoporosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S8756-3282(05)00424-2 DB - PRIME DP - Unbound Medicine ER -