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Brain-derived neurotrophic factor silences GABA synapses onto hypothalamic neuroendocrine cells through a postsynaptic dynamin-mediated mechanism.
J Neurophysiol 2006; 95(4):2193-8JN

Abstract

In the paraventricular nucleus of the hypothalamus (PVN), experimental stress paradigms that suppress gamma-aminobutyric acid (GABA) inputs to parvocellular neuroendocrine cells (PNCs) also increase the expression of brain-derived neurotrophic factor (BDNF). In the adult CNS, BDNF regulates the efficacy of GABAergic transmission, but its contributions to functional changes at inhibitory synapses in the PVN have not been investigated. Analysis of quantal transmission revealed a decrease in the frequency of miniature inhibitory postsynaptic currents (mIPSCs) in response to BDNF with no accompanying changes in their amplitude. These effects were completely blocked by prior inclusion of the TrKB receptor antagonist K252a in the patch pipette. Inclusion of a dynamin inhibitory peptide in the patch pipette also blocked the effects of BDNF, consistent with an all-or-none removal of clusters of postsynaptic GABAA receptors. Finally, to confirm a decrease in the availability of postsynaptic GABAA receptors, we tested the effects of BDNF on focal application of the GABAA agonist muscimol. Postsynaptic responses to muscimol were reduced after BDNF. Collectively, these data indicate that BDNF remodels functional synaptic contacts putatively by reducing the surface expression of postsynaptic GABAA receptors.

Authors+Show Affiliations

Hotchkiss Brain Institute and Department of Physiology and Biophysics, University of Calgary, Calgary, Canada T2N 4N1.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16407427

Citation

Hewitt, Sarah A., and Jaideep S. Bains. "Brain-derived Neurotrophic Factor Silences GABA Synapses Onto Hypothalamic Neuroendocrine Cells Through a Postsynaptic Dynamin-mediated Mechanism." Journal of Neurophysiology, vol. 95, no. 4, 2006, pp. 2193-8.
Hewitt SA, Bains JS. Brain-derived neurotrophic factor silences GABA synapses onto hypothalamic neuroendocrine cells through a postsynaptic dynamin-mediated mechanism. J Neurophysiol. 2006;95(4):2193-8.
Hewitt, S. A., & Bains, J. S. (2006). Brain-derived neurotrophic factor silences GABA synapses onto hypothalamic neuroendocrine cells through a postsynaptic dynamin-mediated mechanism. Journal of Neurophysiology, 95(4), pp. 2193-8.
Hewitt SA, Bains JS. Brain-derived Neurotrophic Factor Silences GABA Synapses Onto Hypothalamic Neuroendocrine Cells Through a Postsynaptic Dynamin-mediated Mechanism. J Neurophysiol. 2006;95(4):2193-8. PubMed PMID: 16407427.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Brain-derived neurotrophic factor silences GABA synapses onto hypothalamic neuroendocrine cells through a postsynaptic dynamin-mediated mechanism. AU - Hewitt,Sarah A, AU - Bains,Jaideep S, Y1 - 2006/01/11/ PY - 2006/1/13/pubmed PY - 2006/9/26/medline PY - 2006/1/13/entrez SP - 2193 EP - 8 JF - Journal of neurophysiology JO - J. Neurophysiol. VL - 95 IS - 4 N2 - In the paraventricular nucleus of the hypothalamus (PVN), experimental stress paradigms that suppress gamma-aminobutyric acid (GABA) inputs to parvocellular neuroendocrine cells (PNCs) also increase the expression of brain-derived neurotrophic factor (BDNF). In the adult CNS, BDNF regulates the efficacy of GABAergic transmission, but its contributions to functional changes at inhibitory synapses in the PVN have not been investigated. Analysis of quantal transmission revealed a decrease in the frequency of miniature inhibitory postsynaptic currents (mIPSCs) in response to BDNF with no accompanying changes in their amplitude. These effects were completely blocked by prior inclusion of the TrKB receptor antagonist K252a in the patch pipette. Inclusion of a dynamin inhibitory peptide in the patch pipette also blocked the effects of BDNF, consistent with an all-or-none removal of clusters of postsynaptic GABAA receptors. Finally, to confirm a decrease in the availability of postsynaptic GABAA receptors, we tested the effects of BDNF on focal application of the GABAA agonist muscimol. Postsynaptic responses to muscimol were reduced after BDNF. Collectively, these data indicate that BDNF remodels functional synaptic contacts putatively by reducing the surface expression of postsynaptic GABAA receptors. SN - 0022-3077 UR - https://www.unboundmedicine.com/medline/citation/16407427/Brain_derived_neurotrophic_factor_silences_GABA_synapses_onto_hypothalamic_neuroendocrine_cells_through_a_postsynaptic_dynamin_mediated_mechanism_ L2 - http://www.physiology.org/doi/full/10.1152/jn.01135.2005?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -