Tags

Type your tag names separated by a space and hit enter

Determination of biogenic amines in HeLa cell lysate by 6-oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl) fluorescein and micellar electrokinetic capillary chromatography with laser-induced fluorescence detection.
Electrophoresis. 2006 Feb; 27(4):827-36.E

Abstract

An MEKC-LIF method using 6-oxy-(N-succinimidyl acetate)-9-(2'-methoxy-carbonyl) fluorescein (SAMF) newly synthesized in our lab as a labeling reagent for the separation and determination of eight typical biogenic amines was proposed. After careful study of the derivatization condition such as pH value, reagent concentration, temperature, and reaction time, derivatization reaction was accomplished as quickly as 10 min with stable yield. Optimal separation of SAMF-labeled amines was achieved with a running buffer (pH 9.3) containing 30 mM boric acid, 25 mM SDS, and 20% v/v ACN. The proposed method allowed biogenic amines to be determined with LODs as low as 0.25-2.5 nmol/L and RSD values from 0.4 to 4.5%. The present method has been successfully used to monitor biogenic amines in HeLa cells and fish samples. This study exploits the potential of MEKC-LIF with SAMF labeling as a tool for monitoring biogenic amines involved in complex physiological and behavioral processes in various matrices.

Authors+Show Affiliations

Department of Chemistry, Wuhan University, Wuhan, PR China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16411271

Citation

Cao, Liwei, et al. "Determination of Biogenic Amines in HeLa Cell Lysate By 6-oxy-(N-succinimidyl Acetate)-9-(2'-methoxycarbonyl) Fluorescein and Micellar Electrokinetic Capillary Chromatography With Laser-induced Fluorescence Detection." Electrophoresis, vol. 27, no. 4, 2006, pp. 827-36.
Cao L, Wang H, Ma M, et al. Determination of biogenic amines in HeLa cell lysate by 6-oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl) fluorescein and micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. Electrophoresis. 2006;27(4):827-36.
Cao, L., Wang, H., Ma, M., & Zhang, H. (2006). Determination of biogenic amines in HeLa cell lysate by 6-oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl) fluorescein and micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. Electrophoresis, 27(4), 827-36.
Cao L, et al. Determination of Biogenic Amines in HeLa Cell Lysate By 6-oxy-(N-succinimidyl Acetate)-9-(2'-methoxycarbonyl) Fluorescein and Micellar Electrokinetic Capillary Chromatography With Laser-induced Fluorescence Detection. Electrophoresis. 2006;27(4):827-36. PubMed PMID: 16411271.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of biogenic amines in HeLa cell lysate by 6-oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl) fluorescein and micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. AU - Cao,Liwei, AU - Wang,Hong, AU - Ma,Ming, AU - Zhang,Huashan, PY - 2006/1/18/pubmed PY - 2006/5/11/medline PY - 2006/1/18/entrez SP - 827 EP - 36 JF - Electrophoresis JO - Electrophoresis VL - 27 IS - 4 N2 - An MEKC-LIF method using 6-oxy-(N-succinimidyl acetate)-9-(2'-methoxy-carbonyl) fluorescein (SAMF) newly synthesized in our lab as a labeling reagent for the separation and determination of eight typical biogenic amines was proposed. After careful study of the derivatization condition such as pH value, reagent concentration, temperature, and reaction time, derivatization reaction was accomplished as quickly as 10 min with stable yield. Optimal separation of SAMF-labeled amines was achieved with a running buffer (pH 9.3) containing 30 mM boric acid, 25 mM SDS, and 20% v/v ACN. The proposed method allowed biogenic amines to be determined with LODs as low as 0.25-2.5 nmol/L and RSD values from 0.4 to 4.5%. The present method has been successfully used to monitor biogenic amines in HeLa cells and fish samples. This study exploits the potential of MEKC-LIF with SAMF labeling as a tool for monitoring biogenic amines involved in complex physiological and behavioral processes in various matrices. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/16411271/Determination_of_biogenic_amines_in_HeLa_cell_lysate_by_6_oxy__N_succinimidyl_acetate__9__2'_methoxycarbonyl__fluorescein_and_micellar_electrokinetic_capillary_chromatography_with_laser_induced_fluorescence_detection_ L2 - https://doi.org/10.1002/elps.200500301 DB - PRIME DP - Unbound Medicine ER -