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Effect of resveratrol on L-type calcium current in rat ventricular myocytes.
Acta Pharmacol Sin. 2006 Feb; 27(2):179-83.AP

Abstract

AIM

To study the effect of resveratrol on L-type calcium current (I(Ca-L)) in isolated rat ventricular myocytes and the mechanisms underlying these effects.

METHODS

I(Ca-L) was examined in isolated single rat ventricular myocytes by using the whole cell patch-clamp recording technique.

RESULTS

Resveratrol (10-40 micromol/L) reduced the peak amplitude of I(Ca-L) and shifted the current-voltage (I-V) curve upwards in a concentration-dependent manner. Resveratrol (10, 20, 40 micromol/L) decreased the peak amplitude of I(Ca-L) from -14.2+/-1.5 pA/pF to -10.5+/-1.5 pA/pF (P<0.05), -7.5+/-2.4 pA/pF (P<0.01), and -5.2+/-1.2 pA/pF (P<0.01), respectively. Resveratrol (40 micromol/L) shifted the steady-state activation curve of I(Ca-L) to the right and changed the half-activation potential (V0.5) from -19.4+/-0.4 mV to -15.4+/-1.9 mV (P<0.05). Resveratrol at a concentration of 40 micromol/L did not affect the steady-state inactivation curve of I(Ca-L), but did markedly shift the time-dependent recovery curve of I(Ca-L) to the right, and slow down the recovery of I(Ca-L) from inactivation. Sodium orthovanadate (Na(3)VO(4); 1 mmol/L), a potent inhibitor of tyrosine phosphatase, significantly inhibited the effects of resveratrol (P<0.01).

CONCLUSION

Resveratrol inhibited I(Ca-L) mainly by inhibiting the activation of L-type calcium channels and slowing down the recovery of L-type calcium channels from inactivation. This inhibitory effect of resveratrol was mediated by the inhibition of protein tyrosine kinase in rat ventricular myocytes.

Authors+Show Affiliations

Zhang LP
Department of Physiology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China.
Yin JX
No affiliation info available
Liu Z
No affiliation info available
Zhang Y
No affiliation info available
Wang QS
No affiliation info available
Zhao J
No affiliation info available

MeSH

AnimalsAnti-Arrhythmia AgentsCalcium Channels, L-TypeDose-Response Relationship, DrugHeart VentriclesMaleMyocytes, CardiacPatch-Clamp TechniquesProtein-Tyrosine KinasesRatsRats, Sprague-DawleyResveratrolStilbenesVanadates

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16412267

Citation

Zhang, Li-ping, et al. "Effect of Resveratrol On L-type Calcium Current in Rat Ventricular Myocytes." Acta Pharmacologica Sinica, vol. 27, no. 2, 2006, pp. 179-83.
Zhang LP, Yin JX, Liu Z, et al. Effect of resveratrol on L-type calcium current in rat ventricular myocytes. Acta Pharmacol Sin. 2006;27(2):179-83.
Zhang, L. P., Yin, J. X., Liu, Z., Zhang, Y., Wang, Q. S., & Zhao, J. (2006). Effect of resveratrol on L-type calcium current in rat ventricular myocytes. Acta Pharmacologica Sinica, 27(2), 179-83.
Zhang LP, et al. Effect of Resveratrol On L-type Calcium Current in Rat Ventricular Myocytes. Acta Pharmacol Sin. 2006;27(2):179-83. PubMed PMID: 16412267.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of resveratrol on L-type calcium current in rat ventricular myocytes. AU - Zhang,Li-ping, AU - Yin,Jing-xiang, AU - Liu,Zheng, AU - Zhang,Yi, AU - Wang,Qing-shan, AU - Zhao,Juan, PY - 2006/1/18/pubmed PY - 2006/9/12/medline PY - 2006/1/18/entrez SP - 179 EP - 83 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 27 IS - 2 N2 - AIM: To study the effect of resveratrol on L-type calcium current (I(Ca-L)) in isolated rat ventricular myocytes and the mechanisms underlying these effects. METHODS: I(Ca-L) was examined in isolated single rat ventricular myocytes by using the whole cell patch-clamp recording technique. RESULTS: Resveratrol (10-40 micromol/L) reduced the peak amplitude of I(Ca-L) and shifted the current-voltage (I-V) curve upwards in a concentration-dependent manner. Resveratrol (10, 20, 40 micromol/L) decreased the peak amplitude of I(Ca-L) from -14.2+/-1.5 pA/pF to -10.5+/-1.5 pA/pF (P<0.05), -7.5+/-2.4 pA/pF (P<0.01), and -5.2+/-1.2 pA/pF (P<0.01), respectively. Resveratrol (40 micromol/L) shifted the steady-state activation curve of I(Ca-L) to the right and changed the half-activation potential (V0.5) from -19.4+/-0.4 mV to -15.4+/-1.9 mV (P<0.05). Resveratrol at a concentration of 40 micromol/L did not affect the steady-state inactivation curve of I(Ca-L), but did markedly shift the time-dependent recovery curve of I(Ca-L) to the right, and slow down the recovery of I(Ca-L) from inactivation. Sodium orthovanadate (Na(3)VO(4); 1 mmol/L), a potent inhibitor of tyrosine phosphatase, significantly inhibited the effects of resveratrol (P<0.01). CONCLUSION: Resveratrol inhibited I(Ca-L) mainly by inhibiting the activation of L-type calcium channels and slowing down the recovery of L-type calcium channels from inactivation. This inhibitory effect of resveratrol was mediated by the inhibition of protein tyrosine kinase in rat ventricular myocytes. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/16412267/Effect_of_resveratrol_on_L_type_calcium_current_in_rat_ventricular_myocytes_ DB - PRIME DP - Unbound Medicine ER -