Tags

Type your tag names separated by a space and hit enter

Analysis of family-level relationships in bees (Hymenoptera: Apiformes) using 28S and two previously unexplored nuclear genes: CAD and RNA polymerase II.
Mol Phylogenet Evol. 2006 May; 39(2):358-72.MP

Abstract

We analyzed a combined data set of two protein-coding nuclear genes (CAD and RNA polymerase II) and a nuclear ribosomal gene (28S D2-D4 region) for 68 bee species and 11 wasp outgroups. Our taxon sampling included all seven extant bee families, 17 of 20 subfamilies, and diverse tribes. Wasp outgroups included the two families most closely related to bees: Crabronidae and Sphecidae. We analyzed the combined and single gene data sets using parsimony and Bayesian methods, which yielded largely congruent results. Our results provide reasonably strong support for family and subfamily-level relationships among bees. Our data set strongly supports the sister-group relationship of the Colletidae and Stenotritidae, and places Halictidae as sister to this clade combined. Our analyses place the Melittidae and the long-tongued (LT) bee clade (Apidae+Megachilidae) near the base of the tree with Colletidae (and Stenotritidae) in a fairly highly derived position. This topology ("Melittidae-LT basal") was obtained in previous morphological studies under certain methods of character coding. A more widely accepted tree topology that places Colletidae (and/or Stenotritidae) as sister to all other bees ("Colletidae basal") is not supported by our data. The "Melittidae-LT basal" hypothesis may better explain patterns in the bee fossil record as well as historical biogeography of certain bee groups. Our results provide new insights into higher-level bee phylogeny and indicate that CAD, RNA polymerase II, and 28S are useful data sets for resolving Cretaceous-age divergences in bees and other Hymenoptera.

Authors+Show Affiliations

Department of Entomology, 3119 Comstock Hall, Cornell University, Ithaca, NY 14853, USA. bnd1@cornell.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16412668

Citation

Danforth, Bryan N., et al. "Analysis of Family-level Relationships in Bees (Hymenoptera: Apiformes) Using 28S and Two Previously Unexplored Nuclear Genes: CAD and RNA Polymerase II." Molecular Phylogenetics and Evolution, vol. 39, no. 2, 2006, pp. 358-72.
Danforth BN, Fang J, Sipes S. Analysis of family-level relationships in bees (Hymenoptera: Apiformes) using 28S and two previously unexplored nuclear genes: CAD and RNA polymerase II. Mol Phylogenet Evol. 2006;39(2):358-72.
Danforth, B. N., Fang, J., & Sipes, S. (2006). Analysis of family-level relationships in bees (Hymenoptera: Apiformes) using 28S and two previously unexplored nuclear genes: CAD and RNA polymerase II. Molecular Phylogenetics and Evolution, 39(2), 358-72.
Danforth BN, Fang J, Sipes S. Analysis of Family-level Relationships in Bees (Hymenoptera: Apiformes) Using 28S and Two Previously Unexplored Nuclear Genes: CAD and RNA Polymerase II. Mol Phylogenet Evol. 2006;39(2):358-72. PubMed PMID: 16412668.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of family-level relationships in bees (Hymenoptera: Apiformes) using 28S and two previously unexplored nuclear genes: CAD and RNA polymerase II. AU - Danforth,Bryan N, AU - Fang,Jennifer, AU - Sipes,Sedonia, Y1 - 2006/01/18/ PY - 2005/07/06/received PY - 2005/09/13/revised PY - 2005/09/20/accepted PY - 2006/1/18/pubmed PY - 2006/7/28/medline PY - 2006/1/18/entrez SP - 358 EP - 72 JF - Molecular phylogenetics and evolution JO - Mol Phylogenet Evol VL - 39 IS - 2 N2 - We analyzed a combined data set of two protein-coding nuclear genes (CAD and RNA polymerase II) and a nuclear ribosomal gene (28S D2-D4 region) for 68 bee species and 11 wasp outgroups. Our taxon sampling included all seven extant bee families, 17 of 20 subfamilies, and diverse tribes. Wasp outgroups included the two families most closely related to bees: Crabronidae and Sphecidae. We analyzed the combined and single gene data sets using parsimony and Bayesian methods, which yielded largely congruent results. Our results provide reasonably strong support for family and subfamily-level relationships among bees. Our data set strongly supports the sister-group relationship of the Colletidae and Stenotritidae, and places Halictidae as sister to this clade combined. Our analyses place the Melittidae and the long-tongued (LT) bee clade (Apidae+Megachilidae) near the base of the tree with Colletidae (and Stenotritidae) in a fairly highly derived position. This topology ("Melittidae-LT basal") was obtained in previous morphological studies under certain methods of character coding. A more widely accepted tree topology that places Colletidae (and/or Stenotritidae) as sister to all other bees ("Colletidae basal") is not supported by our data. The "Melittidae-LT basal" hypothesis may better explain patterns in the bee fossil record as well as historical biogeography of certain bee groups. Our results provide new insights into higher-level bee phylogeny and indicate that CAD, RNA polymerase II, and 28S are useful data sets for resolving Cretaceous-age divergences in bees and other Hymenoptera. SN - 1055-7903 UR - https://www.unboundmedicine.com/medline/citation/16412668/Analysis_of_family_level_relationships_in_bees__Hymenoptera:_Apiformes__using_28S_and_two_previously_unexplored_nuclear_genes:_CAD_and_RNA_polymerase_II_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1055-7903(05)00385-4 DB - PRIME DP - Unbound Medicine ER -