Tags

Type your tag names separated by a space and hit enter

BMP-6 inhibits human bone marrow B lymphopoiesis--upregulation of Id1 and Id3.
Exp Hematol. 2006 Jan; 34(1):72-81.EH

Abstract

OBJECTIVE

In mammals, factors produced by bone marrow (BM) stromal cells are instrumental in orchestrating the developmental process of B lymphocytes. Bone morphogenetic proteins (BMPs) are multifunctional cytokines previously found to regulate hematopoietic stem cells. In the present study, we have explored the role of BMP-6 in human B progenitor cells.

MATERIALS AND METHODS

In vitro B lymphopoiesis of CD10(+) B progenitor cells from human BM was evaluated in the presence or absence of BMP-6 in short- or long-term coculture on MS-5 stromal cells, by tracking CFSE-labeled CD10(+) B progenitor cells or by quantification of CD19(+) cells. DNA synthesis in the pre-B cell line Nalm-6 was measured by (3)H-thymidine incorporation. BMP-6-induced phosphorylation of Smad1/5/8 was determined by Western blot analysis, whereas elevation of Id1-Id4 mRNA levels and basal BMP-6 mRNA levels were measured by real-time and conventional RT-PCR, respectively.

RESULTS

By in vitro coculture of CD10(+) B progenitor cells or monoculture of Nalm-6 cells, we found that BMP-6 inhibited B lymphopoiesis by impeding cell proliferation. Furthermore, in CD10(+) B progenitors as well as in Nalm-6 cells, BMP-6 rapidly induced phosphorylation of Smad1/5/8, followed by an upregulation of Id1 and Id3 mRNA levels. Finally, we demonstrated that human bone marrow stromal cells express BMP-6 mRNA whereas B progenitor cells did not.

CONCLUSIONS

We suggest that BMP-6, produced by the BM, may participate to fine-tune the balance between proliferation, apoptosis, and differentiation in human B progenitor cells during BM B lymphopoiesis.

Authors+Show Affiliations

Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway. christian.kersten@sshf.noNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16413393

Citation

Kersten, Christian, et al. "BMP-6 Inhibits Human Bone Marrow B Lymphopoiesis--upregulation of Id1 and Id3." Experimental Hematology, vol. 34, no. 1, 2006, pp. 72-81.
Kersten C, Dosen G, Myklebust JH, et al. BMP-6 inhibits human bone marrow B lymphopoiesis--upregulation of Id1 and Id3. Exp Hematol. 2006;34(1):72-81.
Kersten, C., Dosen, G., Myklebust, J. H., Sivertsen, E. A., Hystad, M. E., Smeland, E. B., & Rian, E. (2006). BMP-6 inhibits human bone marrow B lymphopoiesis--upregulation of Id1 and Id3. Experimental Hematology, 34(1), 72-81.
Kersten C, et al. BMP-6 Inhibits Human Bone Marrow B Lymphopoiesis--upregulation of Id1 and Id3. Exp Hematol. 2006;34(1):72-81. PubMed PMID: 16413393.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - BMP-6 inhibits human bone marrow B lymphopoiesis--upregulation of Id1 and Id3. AU - Kersten,Christian, AU - Dosen,Guri, AU - Myklebust,June H, AU - Sivertsen,Einar A, AU - Hystad,Marit E, AU - Smeland,Erlend B, AU - Rian,Edith, PY - 2005/06/29/received PY - 2005/09/14/revised PY - 2005/09/14/accepted PY - 2006/1/18/pubmed PY - 2006/3/24/medline PY - 2006/1/18/entrez SP - 72 EP - 81 JF - Experimental hematology JO - Exp Hematol VL - 34 IS - 1 N2 - OBJECTIVE: In mammals, factors produced by bone marrow (BM) stromal cells are instrumental in orchestrating the developmental process of B lymphocytes. Bone morphogenetic proteins (BMPs) are multifunctional cytokines previously found to regulate hematopoietic stem cells. In the present study, we have explored the role of BMP-6 in human B progenitor cells. MATERIALS AND METHODS: In vitro B lymphopoiesis of CD10(+) B progenitor cells from human BM was evaluated in the presence or absence of BMP-6 in short- or long-term coculture on MS-5 stromal cells, by tracking CFSE-labeled CD10(+) B progenitor cells or by quantification of CD19(+) cells. DNA synthesis in the pre-B cell line Nalm-6 was measured by (3)H-thymidine incorporation. BMP-6-induced phosphorylation of Smad1/5/8 was determined by Western blot analysis, whereas elevation of Id1-Id4 mRNA levels and basal BMP-6 mRNA levels were measured by real-time and conventional RT-PCR, respectively. RESULTS: By in vitro coculture of CD10(+) B progenitor cells or monoculture of Nalm-6 cells, we found that BMP-6 inhibited B lymphopoiesis by impeding cell proliferation. Furthermore, in CD10(+) B progenitors as well as in Nalm-6 cells, BMP-6 rapidly induced phosphorylation of Smad1/5/8, followed by an upregulation of Id1 and Id3 mRNA levels. Finally, we demonstrated that human bone marrow stromal cells express BMP-6 mRNA whereas B progenitor cells did not. CONCLUSIONS: We suggest that BMP-6, produced by the BM, may participate to fine-tune the balance between proliferation, apoptosis, and differentiation in human B progenitor cells during BM B lymphopoiesis. SN - 0301-472X UR - https://www.unboundmedicine.com/medline/citation/16413393/BMP_6_inhibits_human_bone_marrow_B_lymphopoiesis__upregulation_of_Id1_and_Id3_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0301-472X(05)00461-3 DB - PRIME DP - Unbound Medicine ER -