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NADPH oxidase is involved in angiotensin II-induced apoptosis in H9C2 cardiac muscle cells: effects of apocynin.
Free Radic Biol Med. 2006 Jan 15; 40(2):236-46.FR

Abstract

Angiotensin II stimulates NADPH oxidase activity in vascular cells. However, it is not fully understood whether angiotensin II, which plays an important role in heart failure, stimulates NADPH oxidase activation and expression in cardiac myocytes. Previous studies have shown that angiotensin II induces myocyte apoptosis, but whether the change is mediated via NADPH oxidase remains to be elucidated. In this study we proposed to determine whether angiotensin II stimulated NADPH oxidase activation and NADPH oxidase subunit p47-phox expression in H9C2 cardiac muscle cells. If so, we would determine whether the NADPH oxidase inhibitor apocynin prevented angiotensin II-induced apoptosis. The results showed that angiotensin II increased NADPH oxidase activity, p47-phox protein and mRNA expression, intracellular reactive oxygen species, and apoptosis in H9C2 cells. Angiotensin II elevated p38 mitogen-activated protein kinase (MAPK) activity, decreased Bcl-2 protein, and increased Bax protein and caspase-3 activity. Apocynin treatment inhibited angiotensin II-induced NADPH oxidase activation and increases in p47-phox expression, intracellular reactive oxygen species, and apoptosis. The effect of apocynin on apoptosis was associated with reduced p38 MAPK activity, increased Bcl-2 protein, and decreased Bax protein and caspase-3 activity. These results suggest that angiotensin II-induced apoptosis is mediated via NADPH oxidase activation probably through p38 MAPK activation, a decrease in Bcl-2 protein, and caspase activation.

Authors+Show Affiliations

Cardiology Unit, Department of Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA. fuzhong-qin@urmc.rochester.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16413406

Citation

Qin, Fuzhong, et al. "NADPH Oxidase Is Involved in Angiotensin II-induced Apoptosis in H9C2 Cardiac Muscle Cells: Effects of Apocynin." Free Radical Biology & Medicine, vol. 40, no. 2, 2006, pp. 236-46.
Qin F, Patel R, Yan C, et al. NADPH oxidase is involved in angiotensin II-induced apoptosis in H9C2 cardiac muscle cells: effects of apocynin. Free Radic Biol Med. 2006;40(2):236-46.
Qin, F., Patel, R., Yan, C., & Liu, W. (2006). NADPH oxidase is involved in angiotensin II-induced apoptosis in H9C2 cardiac muscle cells: effects of apocynin. Free Radical Biology & Medicine, 40(2), 236-46.
Qin F, et al. NADPH Oxidase Is Involved in Angiotensin II-induced Apoptosis in H9C2 Cardiac Muscle Cells: Effects of Apocynin. Free Radic Biol Med. 2006 Jan 15;40(2):236-46. PubMed PMID: 16413406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NADPH oxidase is involved in angiotensin II-induced apoptosis in H9C2 cardiac muscle cells: effects of apocynin. AU - Qin,Fuzhong, AU - Patel,Ravish, AU - Yan,Chen, AU - Liu,Weimin, Y1 - 2005/11/10/ PY - 2004/12/30/received PY - 2005/07/17/revised PY - 2005/08/05/accepted PY - 2006/1/18/pubmed PY - 2006/4/14/medline PY - 2006/1/18/entrez SP - 236 EP - 46 JF - Free radical biology & medicine JO - Free Radic Biol Med VL - 40 IS - 2 N2 - Angiotensin II stimulates NADPH oxidase activity in vascular cells. However, it is not fully understood whether angiotensin II, which plays an important role in heart failure, stimulates NADPH oxidase activation and expression in cardiac myocytes. Previous studies have shown that angiotensin II induces myocyte apoptosis, but whether the change is mediated via NADPH oxidase remains to be elucidated. In this study we proposed to determine whether angiotensin II stimulated NADPH oxidase activation and NADPH oxidase subunit p47-phox expression in H9C2 cardiac muscle cells. If so, we would determine whether the NADPH oxidase inhibitor apocynin prevented angiotensin II-induced apoptosis. The results showed that angiotensin II increased NADPH oxidase activity, p47-phox protein and mRNA expression, intracellular reactive oxygen species, and apoptosis in H9C2 cells. Angiotensin II elevated p38 mitogen-activated protein kinase (MAPK) activity, decreased Bcl-2 protein, and increased Bax protein and caspase-3 activity. Apocynin treatment inhibited angiotensin II-induced NADPH oxidase activation and increases in p47-phox expression, intracellular reactive oxygen species, and apoptosis. The effect of apocynin on apoptosis was associated with reduced p38 MAPK activity, increased Bcl-2 protein, and decreased Bax protein and caspase-3 activity. These results suggest that angiotensin II-induced apoptosis is mediated via NADPH oxidase activation probably through p38 MAPK activation, a decrease in Bcl-2 protein, and caspase activation. SN - 0891-5849 UR - https://www.unboundmedicine.com/medline/citation/16413406/NADPH_oxidase_is_involved_in_angiotensin_II_induced_apoptosis_in_H9C2_cardiac_muscle_cells:_effects_of_apocynin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-5849(05)00452-1 DB - PRIME DP - Unbound Medicine ER -