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Seizure-induced changes in mitochondrial redox status.
Free Radic Biol Med. 2006 Jan 15; 40(2):316-22.FR

Abstract

The aim of this study was to determine seizure-induced oxidative stress by measuring hippocampal glutathione (GSH) and glutathione disulfide (GSSG) levels in tissue and mitochondria. Kainate-induced status epilepticus (SE) in rats resulted in a time-dependent decrease of GSH/GSSG ratios in both hippocampal tissue and mitochondria. However, changes in GSH/GSSG ratios were more dramatic in the mitochondrial fractions compared to hippocampal tissue. This was accompanied by a mild increase in glutathione peroxidase activity and a decrease in glutathione reductase activity in hippocampal tissue and mitochondria, respectively. Since coenzyme A (CoASH) and its disulfide with GSH (CoASSG) are primarily compartmentalized within mitochondria, their measurement in tissue was undertaken to overcome problems associated with GSH/GSSG measurement following subcellular fractionation. Hippocampal tissue CoASH/CoASSG ratios were decreased following kainate-induced SE, the time course and magnitude of change paralleling mitochondrial GSH/GSSG levels. Cysteine, a rate-limiting precursor of glutathione was decreased following kainate administration in both hippocampal tissue and mitochondrial fractions. Together these changes in altered redox status provide further evidence for seizure-induced mitochondrial oxidative stress.

Authors+Show Affiliations

Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Box C238, Denver, CO 80262, USA.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16413413

Citation

Liang, Li-Ping, and Manisha Patel. "Seizure-induced Changes in Mitochondrial Redox Status." Free Radical Biology & Medicine, vol. 40, no. 2, 2006, pp. 316-22.
Liang LP, Patel M. Seizure-induced changes in mitochondrial redox status. Free Radic Biol Med. 2006;40(2):316-22.
Liang, L. P., & Patel, M. (2006). Seizure-induced changes in mitochondrial redox status. Free Radical Biology & Medicine, 40(2), 316-22.
Liang LP, Patel M. Seizure-induced Changes in Mitochondrial Redox Status. Free Radic Biol Med. 2006 Jan 15;40(2):316-22. PubMed PMID: 16413413.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Seizure-induced changes in mitochondrial redox status. AU - Liang,Li-Ping, AU - Patel,Manisha, Y1 - 2005/10/14/ PY - 2005/05/27/received PY - 2005/08/10/revised PY - 2005/08/18/accepted PY - 2006/1/18/pubmed PY - 2006/4/14/medline PY - 2006/1/18/entrez SP - 316 EP - 22 JF - Free radical biology & medicine JO - Free Radic Biol Med VL - 40 IS - 2 N2 - The aim of this study was to determine seizure-induced oxidative stress by measuring hippocampal glutathione (GSH) and glutathione disulfide (GSSG) levels in tissue and mitochondria. Kainate-induced status epilepticus (SE) in rats resulted in a time-dependent decrease of GSH/GSSG ratios in both hippocampal tissue and mitochondria. However, changes in GSH/GSSG ratios were more dramatic in the mitochondrial fractions compared to hippocampal tissue. This was accompanied by a mild increase in glutathione peroxidase activity and a decrease in glutathione reductase activity in hippocampal tissue and mitochondria, respectively. Since coenzyme A (CoASH) and its disulfide with GSH (CoASSG) are primarily compartmentalized within mitochondria, their measurement in tissue was undertaken to overcome problems associated with GSH/GSSG measurement following subcellular fractionation. Hippocampal tissue CoASH/CoASSG ratios were decreased following kainate-induced SE, the time course and magnitude of change paralleling mitochondrial GSH/GSSG levels. Cysteine, a rate-limiting precursor of glutathione was decreased following kainate administration in both hippocampal tissue and mitochondrial fractions. Together these changes in altered redox status provide further evidence for seizure-induced mitochondrial oxidative stress. SN - 0891-5849 UR - https://www.unboundmedicine.com/medline/citation/16413413/Seizure_induced_changes_in_mitochondrial_redox_status_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-5849(05)00487-9 DB - PRIME DP - Unbound Medicine ER -