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Oral insulin enhances intestinal regrowth following massive small bowel resection in rat.
Dig Dis Sci. 2005 Dec; 50(12):2379-85.DD

Abstract

Experimental studies have suggested that insulin (INS) plays an important role in small intestinal growth and development. In the present study we investigated the effect of oral INS on structural intestinal adaptation and enterocyte proliferation and loss via apoptosis in a rat model of short bowel syndrome (SBS). Male Sprague-Dawley rats were divided into three experimental groups: sham rats underwent bowel transection, SBS rats underwent 75% small bowel resection, and SBS-INS rats underwent bowel resection and were treated with oral INS given in the drinking water from the 3rd to the 15th postoperative day. Parameters of intestinal adaptation (bowel and mucosal weight, mucosal DNA and protein, villous height, and crypt depth), enterocyte proliferation, and apoptosis were determined on day 15. SBS-INS rats demonstrated a significant increase (vs SBS rats) in jejunal and ileal overall bowel and mucosal weight, ileal mucosal DNA and protein, ileal villous height, and crypt depth. SBS-INS rats also showed an increased cell proliferation index in jejunum and ileum and decreased apoptotic index in jejunum compared to SBS animals. In conclusion, in a rat model of SBS, oral INS strongly enhances intestinal adaptation. Possible mechanisms may include increased cell proliferation and decreased enterocyte loss via apoptosis.

Authors+Show Affiliations

Rappaport Faculty of Medicine, Technion, Department of Pediatric Surgery B, Bnai Zion Medical Center, Haifa, Israel. igor-dr@internet-zahav.netNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16416194

Citation

Sukhotnik, Igor, et al. "Oral Insulin Enhances Intestinal Regrowth Following Massive Small Bowel Resection in Rat." Digestive Diseases and Sciences, vol. 50, no. 12, 2005, pp. 2379-85.
Sukhotnik I, Shehadeh N, Shamir R, et al. Oral insulin enhances intestinal regrowth following massive small bowel resection in rat. Dig Dis Sci. 2005;50(12):2379-85.
Sukhotnik, I., Shehadeh, N., Shamir, R., Bejar, J., Bernshteyn, A., & Mogilner, J. G. (2005). Oral insulin enhances intestinal regrowth following massive small bowel resection in rat. Digestive Diseases and Sciences, 50(12), 2379-85.
Sukhotnik I, et al. Oral Insulin Enhances Intestinal Regrowth Following Massive Small Bowel Resection in Rat. Dig Dis Sci. 2005;50(12):2379-85. PubMed PMID: 16416194.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oral insulin enhances intestinal regrowth following massive small bowel resection in rat. AU - Sukhotnik,Igor, AU - Shehadeh,Naim, AU - Shamir,Raanan, AU - Bejar,Jacob, AU - Bernshteyn,Aleksander, AU - Mogilner,Jorge G, PY - 2004/12/07/received PY - 2005/02/08/accepted PY - 2006/1/18/pubmed PY - 2006/2/4/medline PY - 2006/1/18/entrez SP - 2379 EP - 85 JF - Digestive diseases and sciences JO - Dig Dis Sci VL - 50 IS - 12 N2 - Experimental studies have suggested that insulin (INS) plays an important role in small intestinal growth and development. In the present study we investigated the effect of oral INS on structural intestinal adaptation and enterocyte proliferation and loss via apoptosis in a rat model of short bowel syndrome (SBS). Male Sprague-Dawley rats were divided into three experimental groups: sham rats underwent bowel transection, SBS rats underwent 75% small bowel resection, and SBS-INS rats underwent bowel resection and were treated with oral INS given in the drinking water from the 3rd to the 15th postoperative day. Parameters of intestinal adaptation (bowel and mucosal weight, mucosal DNA and protein, villous height, and crypt depth), enterocyte proliferation, and apoptosis were determined on day 15. SBS-INS rats demonstrated a significant increase (vs SBS rats) in jejunal and ileal overall bowel and mucosal weight, ileal mucosal DNA and protein, ileal villous height, and crypt depth. SBS-INS rats also showed an increased cell proliferation index in jejunum and ileum and decreased apoptotic index in jejunum compared to SBS animals. In conclusion, in a rat model of SBS, oral INS strongly enhances intestinal adaptation. Possible mechanisms may include increased cell proliferation and decreased enterocyte loss via apoptosis. SN - 0163-2116 UR - https://www.unboundmedicine.com/medline/citation/16416194/Oral_insulin_enhances_intestinal_regrowth_following_massive_small_bowel_resection_in_rat_ DB - PRIME DP - Unbound Medicine ER -