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Study on the antiinflammatory activity of methanol extract from seagrass Zostera japonica.
J Agric Food Chem. 2006 Jan 25; 54(2):306-11.JA

Abstract

Methanolic extracts from the seagrass Zostera japonica were extracted successively using n-hexane (n-C(6)H(14)), dichloromethane (CH(2)Cl(2)), ethyl acetate (EtOAc), and water to give the n-C(6)H(14) (16.8%), CH(2)Cl(2) (40.6%), EtOAc (34.1%), and H(2)O (8.5%) soluble fractions, respectively. We have demonstrated that the hexane fraction has the highest capacity to inhibit proIL-1beta expression as compared to other fractions in lipopolysaccharides (LPS)-stimulated J774A.1 murine macrophages. Further analysis of the composition and antiinflammatory activity of the subfraction H5 from hexane fraction showed that it had the best antiinflammatory capacity and that it's major constituents were fatty acids, including palmitic acid methyl ester (21.5%), palmitic acid (24.02%), linoleic acid methyl ester (13.09%), oleic acid methyl ester (8.41%), and linoleic acid (7.93%), respectively. H5 inhibited LPS-induced TNFalpha, IL-1beta, and IL-6 in a dose-dependent manner, suggesting that H5 is bioactive in antiinflammation in vitro. This study is the first to report the antiinflammatory activity of extracts obtained from the seagrass Z. japonica.

Authors+Show Affiliations

Hua KF
Faculty of Biotechnology and Laboratory Science in Medicine, Institute of Biotechnology in Medicine, National Yang-Ming University, Taipei 112, Taiwan.
Hsu HY
No affiliation info available
Su YC
No affiliation info available
Lin IF
No affiliation info available
Yang SS
No affiliation info available
Chen YM
No affiliation info available
Chao LK
No affiliation info available

MeSH

AnimalsAnti-Inflammatory AgentsBlotting, WesternCell LineGas Chromatography-Mass SpectrometryHexanesInterleukin-1Interleukin-6LipopolysaccharidesMacrophagesMethanolMicePlant ExtractsTumor Necrosis Factor-alphaZosteraceae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16417284

Citation

Hua, Kuo-Feng, et al. "Study On the Antiinflammatory Activity of Methanol Extract From Seagrass Zostera Japonica." Journal of Agricultural and Food Chemistry, vol. 54, no. 2, 2006, pp. 306-11.
Hua KF, Hsu HY, Su YC, et al. Study on the antiinflammatory activity of methanol extract from seagrass Zostera japonica. J Agric Food Chem. 2006;54(2):306-11.
Hua, K. F., Hsu, H. Y., Su, Y. C., Lin, I. F., Yang, S. S., Chen, Y. M., & Chao, L. K. (2006). Study on the antiinflammatory activity of methanol extract from seagrass Zostera japonica. Journal of Agricultural and Food Chemistry, 54(2), 306-11.
Hua KF, et al. Study On the Antiinflammatory Activity of Methanol Extract From Seagrass Zostera Japonica. J Agric Food Chem. 2006 Jan 25;54(2):306-11. PubMed PMID: 16417284.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Study on the antiinflammatory activity of methanol extract from seagrass Zostera japonica. AU - Hua,Kuo-Feng, AU - Hsu,Hsien-Yeh, AU - Su,Yu-Chang, AU - Lin,I-Fan, AU - Yang,Su-Sing, AU - Chen,Ya-Mai, AU - Chao,Louis Kuoping, PY - 2006/1/19/pubmed PY - 2006/3/29/medline PY - 2006/1/19/entrez SP - 306 EP - 11 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 54 IS - 2 N2 - Methanolic extracts from the seagrass Zostera japonica were extracted successively using n-hexane (n-C(6)H(14)), dichloromethane (CH(2)Cl(2)), ethyl acetate (EtOAc), and water to give the n-C(6)H(14) (16.8%), CH(2)Cl(2) (40.6%), EtOAc (34.1%), and H(2)O (8.5%) soluble fractions, respectively. We have demonstrated that the hexane fraction has the highest capacity to inhibit proIL-1beta expression as compared to other fractions in lipopolysaccharides (LPS)-stimulated J774A.1 murine macrophages. Further analysis of the composition and antiinflammatory activity of the subfraction H5 from hexane fraction showed that it had the best antiinflammatory capacity and that it's major constituents were fatty acids, including palmitic acid methyl ester (21.5%), palmitic acid (24.02%), linoleic acid methyl ester (13.09%), oleic acid methyl ester (8.41%), and linoleic acid (7.93%), respectively. H5 inhibited LPS-induced TNFalpha, IL-1beta, and IL-6 in a dose-dependent manner, suggesting that H5 is bioactive in antiinflammation in vitro. This study is the first to report the antiinflammatory activity of extracts obtained from the seagrass Z. japonica. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16417284/Study_on_the_antiinflammatory_activity_of_methanol_extract_from_seagrass_Zostera_japonica_ DB - PRIME DP - Unbound Medicine ER -