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Detection of cannabis use in drivers with the drugwipe device and by GC-MS after Intercept device collection.
J Anal Toxicol. 2005 Oct; 29(7):724-7.JA

Abstract

Saliva or "oral fluid" has been presented as an alternative matrix in the establishment of drug exposure. The noninvasive collection of a saliva sample, which is relatively easy to perform and can be achieved under close supervision, is one of the most important benefits in a driving under the influence situation. Moreover, the presence of delta9-tetrahydrocannabinol (THC) in oral fluid is a better indication of recent use than when the drug is detected in urine, so there is a higher probability that the subject is experiencing pharmacological effects at the time of sampling. At 3 check points organized by the Swiss police in Bern, 61 drivers were tested for the presence of drugs of abuse using the Drugwipe 5 device. In parallel, oral fluid was collected with the Intercept DOA Oral Specimen Collection device and tested by gas chromatography-mass spectrometry (GC-MS) after methylation of THC (limit of quantitation 1 ng/mL). The Drugwipe device identified 1 exposed driver, but with GC-MS, 18 drivers tested positive. THC concentrations in the Intercept buffer ranged from 2.1 to 205.1 ng/mL. These concentrations represent about 1/2 to 1/3 the authentic THC concentrations in oral fluid because of the dilution by the blue liquid of the device. Two main limitations of oral fluid were 1. the amount of matrix collected is smaller when compared to urine and 2. the levels of drugs in urine are higher than in oral fluid. A current limitation of the use of this specimen for roadside testing is the absence of a suitable immunoassay that detects the parent compound in sufficiently low concentrations.

Authors+Show Affiliations

X'pertise Consulting, Laboratoire ChemTox, 3, rue Grüninger, F-67400 Illkirch, France. pascal.kintz@wanadoo.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16419408

Citation

Kintz, Pascal, et al. "Detection of Cannabis Use in Drivers With the Drugwipe Device and By GC-MS After Intercept Device Collection." Journal of Analytical Toxicology, vol. 29, no. 7, 2005, pp. 724-7.
Kintz P, Bernhard W, Villain M, et al. Detection of cannabis use in drivers with the drugwipe device and by GC-MS after Intercept device collection. J Anal Toxicol. 2005;29(7):724-7.
Kintz, P., Bernhard, W., Villain, M., Gasser, M., Aebi, B., & Cirimele, V. (2005). Detection of cannabis use in drivers with the drugwipe device and by GC-MS after Intercept device collection. Journal of Analytical Toxicology, 29(7), 724-7.
Kintz P, et al. Detection of Cannabis Use in Drivers With the Drugwipe Device and By GC-MS After Intercept Device Collection. J Anal Toxicol. 2005;29(7):724-7. PubMed PMID: 16419408.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of cannabis use in drivers with the drugwipe device and by GC-MS after Intercept device collection. AU - Kintz,Pascal, AU - Bernhard,Werner, AU - Villain,Marion, AU - Gasser,Martina, AU - Aebi,Beat, AU - Cirimele,Vincent, PY - 2006/1/20/pubmed PY - 2006/2/4/medline PY - 2006/1/20/entrez SP - 724 EP - 7 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 29 IS - 7 N2 - Saliva or "oral fluid" has been presented as an alternative matrix in the establishment of drug exposure. The noninvasive collection of a saliva sample, which is relatively easy to perform and can be achieved under close supervision, is one of the most important benefits in a driving under the influence situation. Moreover, the presence of delta9-tetrahydrocannabinol (THC) in oral fluid is a better indication of recent use than when the drug is detected in urine, so there is a higher probability that the subject is experiencing pharmacological effects at the time of sampling. At 3 check points organized by the Swiss police in Bern, 61 drivers were tested for the presence of drugs of abuse using the Drugwipe 5 device. In parallel, oral fluid was collected with the Intercept DOA Oral Specimen Collection device and tested by gas chromatography-mass spectrometry (GC-MS) after methylation of THC (limit of quantitation 1 ng/mL). The Drugwipe device identified 1 exposed driver, but with GC-MS, 18 drivers tested positive. THC concentrations in the Intercept buffer ranged from 2.1 to 205.1 ng/mL. These concentrations represent about 1/2 to 1/3 the authentic THC concentrations in oral fluid because of the dilution by the blue liquid of the device. Two main limitations of oral fluid were 1. the amount of matrix collected is smaller when compared to urine and 2. the levels of drugs in urine are higher than in oral fluid. A current limitation of the use of this specimen for roadside testing is the absence of a suitable immunoassay that detects the parent compound in sufficiently low concentrations. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/16419408/Detection_of_cannabis_use_in_drivers_with_the_drugwipe_device_and_by_GC_MS_after_Intercept_device_collection_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/29.7.724 DB - PRIME DP - Unbound Medicine ER -