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The hairless guinea-pig as a model for treatment of cumulative irritation in humans.
Skin Res Technol. 2006 Feb; 12(1):60-7.SR

Abstract

BACKGROUND

The effect of six skin-care formulations (SCFs) on experimentally induced cumulative irritation was studied in hairless guinea-pigs (HLGPs) and in human volunteers (HVs). The formulations were a basic cream, a carbomer cream and four modifications of the carbomer cream, containing either 10% isopropyl palmitate (IPP cream), 10% glycerol (glycerol cream), 19.5% canola oil (canola oil cream) or 0.5% (-)-alpha-bisabolol (bisabolol cream).

METHODS

In HLGP, irritant dermatitis was induced with 30 min daily exposure for 4 days to 0.5% sodium lauryl sulfate aq. (SLS). In HVs, irritant dermatitis was induced with 10 min daily exposure for 5+4 days (no irritation on weekends) to 3% SLS aq. on the right and 30% nonanoic acid (NON) in n-propanol on the left volar forearm. Clinical scoring was performed daily; evaporimetry (total epidermal water loss (TEWL)), hydration and colorimetry were measured at baseline (day 0) in the middle and at the end of treatment. Treatments were applied twice daily. The basic cream and the IPP cream were excluded from testing in HLGP because they were known from previous studies to be irritant in HLGP, while all formulations were known to be equally and well tolerated locally in humans.

RESULTS

All formulations worsened the skin irritation in HLGP: the glycerol cream the least, the canola oil cream the most, while the bisabolol cream and the carbomer cream were indistinguishable. In humans, the glycerol cream was better than 'No Treatment' after cumulative irritation with both SLS and NON. The basic cream was better tolerated in humans than was expected from previous testing in HLGPs.

CONCLUSION

In conclusion, the results from the studies in HLGPs and HVs are in agreement with regard to ranking of the SCFs. Further, the glycerol cream showed a positive treatment effect on both SLS- and NON-irritated skin in HVs.

Authors+Show Affiliations

Department of Dermatology and Allergy Center, Odense University Hospital, University of Southern Denmark, Odense, Denmark. flemming.andersen@ouh.fyns-amt.dkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article

Language

eng

PubMed ID

16420540

Citation

Andersen, F, et al. "The Hairless Guinea-pig as a Model for Treatment of Cumulative Irritation in Humans." Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI), vol. 12, no. 1, 2006, pp. 60-7.
Andersen F, Hedegaard K, Petersen TK, et al. The hairless guinea-pig as a model for treatment of cumulative irritation in humans. Skin Res Technol. 2006;12(1):60-7.
Andersen, F., Hedegaard, K., Petersen, T. K., Bindslev-Jensen, C., Fullerton, A., & Andersen, K. E. (2006). The hairless guinea-pig as a model for treatment of cumulative irritation in humans. Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI), 12(1), 60-7.
Andersen F, et al. The Hairless Guinea-pig as a Model for Treatment of Cumulative Irritation in Humans. Skin Res Technol. 2006;12(1):60-7. PubMed PMID: 16420540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The hairless guinea-pig as a model for treatment of cumulative irritation in humans. AU - Andersen,F, AU - Hedegaard,K, AU - Petersen,T K, AU - Bindslev-Jensen,C, AU - Fullerton,A, AU - Andersen,K E, PY - 2006/1/20/pubmed PY - 2006/3/30/medline PY - 2006/1/20/entrez SP - 60 EP - 7 JF - Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) JO - Skin Res Technol VL - 12 IS - 1 N2 - BACKGROUND: The effect of six skin-care formulations (SCFs) on experimentally induced cumulative irritation was studied in hairless guinea-pigs (HLGPs) and in human volunteers (HVs). The formulations were a basic cream, a carbomer cream and four modifications of the carbomer cream, containing either 10% isopropyl palmitate (IPP cream), 10% glycerol (glycerol cream), 19.5% canola oil (canola oil cream) or 0.5% (-)-alpha-bisabolol (bisabolol cream). METHODS: In HLGP, irritant dermatitis was induced with 30 min daily exposure for 4 days to 0.5% sodium lauryl sulfate aq. (SLS). In HVs, irritant dermatitis was induced with 10 min daily exposure for 5+4 days (no irritation on weekends) to 3% SLS aq. on the right and 30% nonanoic acid (NON) in n-propanol on the left volar forearm. Clinical scoring was performed daily; evaporimetry (total epidermal water loss (TEWL)), hydration and colorimetry were measured at baseline (day 0) in the middle and at the end of treatment. Treatments were applied twice daily. The basic cream and the IPP cream were excluded from testing in HLGP because they were known from previous studies to be irritant in HLGP, while all formulations were known to be equally and well tolerated locally in humans. RESULTS: All formulations worsened the skin irritation in HLGP: the glycerol cream the least, the canola oil cream the most, while the bisabolol cream and the carbomer cream were indistinguishable. In humans, the glycerol cream was better than 'No Treatment' after cumulative irritation with both SLS and NON. The basic cream was better tolerated in humans than was expected from previous testing in HLGPs. CONCLUSION: In conclusion, the results from the studies in HLGPs and HVs are in agreement with regard to ranking of the SCFs. Further, the glycerol cream showed a positive treatment effect on both SLS- and NON-irritated skin in HVs. SN - 0909-752X UR - https://www.unboundmedicine.com/medline/citation/16420540/The_hairless_guinea_pig_as_a_model_for_treatment_of_cumulative_irritation_in_humans_ DB - PRIME DP - Unbound Medicine ER -