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Flow cytometry-based method to analyze the change in Tau phosphorylation in a hGSK-3beta and hTau over-expressing EcR-293 cell line.
Neurochem Int. 2006 Apr; 48(5):374-82.NI

Abstract

Neurofibrillary tangles are composed of insoluble aggregates of microtubule-associated protein Tau. In the pathology of Alzheimer's disease (AD), accumulation of hyperphosphorylated Tau results in formation of paired helical filaments. One of the main candidate to hyperphosphorylate Tau in AD is glycogen synthase kinase 3beta (GSK-3beta). Here we introduce a non-neuronal cell line, stably co-expressing human Tau and GSK-3beta proteins, where the effect of potential kinase inhibitors on Tau phosphorylation can be monitored. The aim of our study was to establish a new flow-cytometry-based method to quantitatively analyze the changing of Tau phosphorylation, which is a suitable alternative to the well-accepted but non-quantitative Western blot technique. Our results demonstrate that the flow cytometry-based method is a convenient tool to analyze the effect of GSK-3beta inhibitors on Tau phosphorylation. This new approach provides appropriate throughput for screening purposes in preclinical research for characterization of GSK-3beta inhibitors, as potential drug candidate to cure Alzheimer's disease.

Authors+Show Affiliations

Kurko D
Pharmacological and Drug Safety Research, Gedeon Richter Ltd., Budapest, Hungary.
Boros A
No affiliation info available
Dezso P
No affiliation info available
Urbányi Z
No affiliation info available
Sárvári M
No affiliation info available
Nagy J
No affiliation info available
Szombathelyi Z
No affiliation info available
Szendrei GI
No affiliation info available

MeSH

Alzheimer DiseaseCell LineDrug Evaluation, PreclinicalEnzyme InhibitorsFlow CytometryGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaHumansNeurofibrillary TanglesPhosphorylationRNA, MessengerUp-Regulationtau Proteins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16420965

Citation

Kurko, Dalma, et al. "Flow Cytometry-based Method to Analyze the Change in Tau Phosphorylation in a hGSK-3beta and hTau Over-expressing EcR-293 Cell Line." Neurochemistry International, vol. 48, no. 5, 2006, pp. 374-82.
Kurko D, Boros A, Dezso P, et al. Flow cytometry-based method to analyze the change in Tau phosphorylation in a hGSK-3beta and hTau over-expressing EcR-293 cell line. Neurochem Int. 2006;48(5):374-82.
Kurko, D., Boros, A., Dezso, P., Urbányi, Z., Sárvári, M., Nagy, J., Szombathelyi, Z., & Szendrei, G. I. (2006). Flow cytometry-based method to analyze the change in Tau phosphorylation in a hGSK-3beta and hTau over-expressing EcR-293 cell line. Neurochemistry International, 48(5), 374-82.
Kurko D, et al. Flow Cytometry-based Method to Analyze the Change in Tau Phosphorylation in a hGSK-3beta and hTau Over-expressing EcR-293 Cell Line. Neurochem Int. 2006;48(5):374-82. PubMed PMID: 16420965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flow cytometry-based method to analyze the change in Tau phosphorylation in a hGSK-3beta and hTau over-expressing EcR-293 cell line. AU - Kurko,Dalma, AU - Boros,András, AU - Dezso,Péter, AU - Urbányi,Zoltán, AU - Sárvári,Miklós, AU - Nagy,József, AU - Szombathelyi,Zsolt, AU - Szendrei,Györgyi I, Y1 - 2006/01/18/ PY - 2005/08/25/received PY - 2005/11/18/accepted PY - 2006/1/20/pubmed PY - 2006/6/2/medline PY - 2006/1/20/entrez SP - 374 EP - 82 JF - Neurochemistry international JO - Neurochem Int VL - 48 IS - 5 N2 - Neurofibrillary tangles are composed of insoluble aggregates of microtubule-associated protein Tau. In the pathology of Alzheimer's disease (AD), accumulation of hyperphosphorylated Tau results in formation of paired helical filaments. One of the main candidate to hyperphosphorylate Tau in AD is glycogen synthase kinase 3beta (GSK-3beta). Here we introduce a non-neuronal cell line, stably co-expressing human Tau and GSK-3beta proteins, where the effect of potential kinase inhibitors on Tau phosphorylation can be monitored. The aim of our study was to establish a new flow-cytometry-based method to quantitatively analyze the changing of Tau phosphorylation, which is a suitable alternative to the well-accepted but non-quantitative Western blot technique. Our results demonstrate that the flow cytometry-based method is a convenient tool to analyze the effect of GSK-3beta inhibitors on Tau phosphorylation. This new approach provides appropriate throughput for screening purposes in preclinical research for characterization of GSK-3beta inhibitors, as potential drug candidate to cure Alzheimer's disease. SN - 0197-0186 UR - https://www.unboundmedicine.com/medline/citation/16420965/Flow_cytometry_based_method_to_analyze_the_change_in_Tau_phosphorylation_in_a_hGSK_3beta_and_hTau_over_expressing_EcR_293_cell_line_ DB - PRIME DP - Unbound Medicine ER -