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Effects of a chronic compression of the dorsal root ganglion on voltage-gated Na+ and K+ currents in cutaneous afferent neurons.
J Neurophysiol. 2006 Feb; 95(2):1115-23.JN

Abstract

A chronic compression of the dorsal root ganglion (CCD) produces ipsilateral cutaneous hyperalgesia that is associated with an increased excitability of neuronal somata in the compressed ganglion, as evidenced by spontaneous activity and a lower rheobase. We searched for differences in the properties of voltage-gated Na+ and K+ currents between somata of CCD- and control (unoperated) rats. CCD was produced in adult rats by inserting two rods through the intervertebral foramina, one compressing the L4, and the other, the ipsilateral, L5 dorsal root ganglion (DRG). After 5-9 days, DRG somata were dissociated and placed in culture for 16-26 h. Cutaneous neurons of medium size (35-45 microm), Fluorogold-labeled from the hindpaw, were selected for whole cell patch-clamp recording of action potentials and ion currents. In comparison with control neurons, CCD neurons had steady-state activation curves for TTX-sensitive (TTX-S) Na+ currents that were shifted in the hyperpolarizing direction, and CCD neurons had enhanced TTX-resistant (TTX-R) current. CCD neurons also had smaller, fast-inactivating K+ currents (Ka) at voltages from -30 to 50 mV. The reduction in Ka, the hyperpolarizing shift in TTX-S Na+ current activation, and the enhanced TTX-R Na+ current may all contribute to the enhanced neuronal excitability and thus to the pain and hyperalgesia associated with CCD.

Authors+Show Affiliations

Department of Anesthesiology, Yale University School of Medicine, New Haven, CT 06510, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16424456

Citation

Tan, Z Y., et al. "Effects of a Chronic Compression of the Dorsal Root Ganglion On Voltage-gated Na+ and K+ Currents in Cutaneous Afferent Neurons." Journal of Neurophysiology, vol. 95, no. 2, 2006, pp. 1115-23.
Tan ZY, Donnelly DF, LaMotte RH. Effects of a chronic compression of the dorsal root ganglion on voltage-gated Na+ and K+ currents in cutaneous afferent neurons. J Neurophysiol. 2006;95(2):1115-23.
Tan, Z. Y., Donnelly, D. F., & LaMotte, R. H. (2006). Effects of a chronic compression of the dorsal root ganglion on voltage-gated Na+ and K+ currents in cutaneous afferent neurons. Journal of Neurophysiology, 95(2), 1115-23.
Tan ZY, Donnelly DF, LaMotte RH. Effects of a Chronic Compression of the Dorsal Root Ganglion On Voltage-gated Na+ and K+ Currents in Cutaneous Afferent Neurons. J Neurophysiol. 2006;95(2):1115-23. PubMed PMID: 16424456.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of a chronic compression of the dorsal root ganglion on voltage-gated Na+ and K+ currents in cutaneous afferent neurons. AU - Tan,Z Y, AU - Donnelly,D F, AU - LaMotte,R H, PY - 2006/1/21/pubmed PY - 2006/3/1/medline PY - 2006/1/21/entrez SP - 1115 EP - 23 JF - Journal of neurophysiology JO - J Neurophysiol VL - 95 IS - 2 N2 - A chronic compression of the dorsal root ganglion (CCD) produces ipsilateral cutaneous hyperalgesia that is associated with an increased excitability of neuronal somata in the compressed ganglion, as evidenced by spontaneous activity and a lower rheobase. We searched for differences in the properties of voltage-gated Na+ and K+ currents between somata of CCD- and control (unoperated) rats. CCD was produced in adult rats by inserting two rods through the intervertebral foramina, one compressing the L4, and the other, the ipsilateral, L5 dorsal root ganglion (DRG). After 5-9 days, DRG somata were dissociated and placed in culture for 16-26 h. Cutaneous neurons of medium size (35-45 microm), Fluorogold-labeled from the hindpaw, were selected for whole cell patch-clamp recording of action potentials and ion currents. In comparison with control neurons, CCD neurons had steady-state activation curves for TTX-sensitive (TTX-S) Na+ currents that were shifted in the hyperpolarizing direction, and CCD neurons had enhanced TTX-resistant (TTX-R) current. CCD neurons also had smaller, fast-inactivating K+ currents (Ka) at voltages from -30 to 50 mV. The reduction in Ka, the hyperpolarizing shift in TTX-S Na+ current activation, and the enhanced TTX-R Na+ current may all contribute to the enhanced neuronal excitability and thus to the pain and hyperalgesia associated with CCD. SN - 0022-3077 UR - https://www.unboundmedicine.com/medline/citation/16424456/Effects_of_a_chronic_compression_of_the_dorsal_root_ganglion_on_voltage_gated_Na+_and_K+_currents_in_cutaneous_afferent_neurons_ L2 - https://journals.physiology.org/doi/10.1152/jn.00830.2005?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -