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In vitro antioxidant activity of Diospyros malabarica Kostel bark.
Indian J Exp Biol. 2006 Jan; 44(1):39-44.IJ

Abstract

Antioxidant activity of defatted methanol extract of D. malabarica bark was studied for its free radical scavenging property on different in vitro models e.g. 1,1-diphenyl-2-picryl hydrazyl (DPPH), nitric oxide, superoxide, hydroxyl radical and lipid peroxide radical model. The extract showed good dose-dependent free radical scavenging property in all the models except in hydroxyl radical inhibition assay. IC50 values were found to be 9.16, 13.21, 25.27 and 17.33 microg/ml respectively in DPPH, nitric oxide, superoxide and lipid peroxidation inhibition assays. In hydroxyl radical inhibition assay 1000 microg/ml extract showed only 10% inhibition with respect to the control. Measurement of total phenolic compounds by Folin-Ciocalteu's phenol reagent indicated that 1 mg of the extract contained 120.7 microg equivalent of pyrocatechol. The results indicate that the antioxidant property of the extract may be due to the high content of phenolic compounds. However, the underlying mechanism may not involve hydroxyl radical scavenging property.

Authors+Show Affiliations

Mondal SK
Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700 032, India. susantam@india.com
Chakraborty G
No affiliation info available
Gupta M
No affiliation info available
Mazumder UK
No affiliation info available

MeSH

AnimalsAntioxidantsDiospyrosFemaleFree Radical ScavengersIn Vitro TechniquesLipid PeroxidationLiverMaleMicePlant ExtractsRatsRats, Wistar

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16430089

Citation

Mondal, Susanta Kumar, et al. "In Vitro Antioxidant Activity of Diospyros Malabarica Kostel Bark." Indian Journal of Experimental Biology, vol. 44, no. 1, 2006, pp. 39-44.
Mondal SK, Chakraborty G, Gupta M, et al. In vitro antioxidant activity of Diospyros malabarica Kostel bark. Indian J Exp Biol. 2006;44(1):39-44.
Mondal, S. K., Chakraborty, G., Gupta, M., & Mazumder, U. K. (2006). In vitro antioxidant activity of Diospyros malabarica Kostel bark. Indian Journal of Experimental Biology, 44(1), 39-44.
Mondal SK, et al. In Vitro Antioxidant Activity of Diospyros Malabarica Kostel Bark. Indian J Exp Biol. 2006;44(1):39-44. PubMed PMID: 16430089.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antioxidant activity of Diospyros malabarica Kostel bark. AU - Mondal,Susanta Kumar, AU - Chakraborty,Goutam, AU - Gupta,M, AU - Mazumder,U K, PY - 2006/1/25/pubmed PY - 2006/3/3/medline PY - 2006/1/25/entrez SP - 39 EP - 44 JF - Indian journal of experimental biology JO - Indian J Exp Biol VL - 44 IS - 1 N2 - Antioxidant activity of defatted methanol extract of D. malabarica bark was studied for its free radical scavenging property on different in vitro models e.g. 1,1-diphenyl-2-picryl hydrazyl (DPPH), nitric oxide, superoxide, hydroxyl radical and lipid peroxide radical model. The extract showed good dose-dependent free radical scavenging property in all the models except in hydroxyl radical inhibition assay. IC50 values were found to be 9.16, 13.21, 25.27 and 17.33 microg/ml respectively in DPPH, nitric oxide, superoxide and lipid peroxidation inhibition assays. In hydroxyl radical inhibition assay 1000 microg/ml extract showed only 10% inhibition with respect to the control. Measurement of total phenolic compounds by Folin-Ciocalteu's phenol reagent indicated that 1 mg of the extract contained 120.7 microg equivalent of pyrocatechol. The results indicate that the antioxidant property of the extract may be due to the high content of phenolic compounds. However, the underlying mechanism may not involve hydroxyl radical scavenging property. SN - 0019-5189 UR - https://www.unboundmedicine.com/medline/citation/16430089/In_vitro_antioxidant_activity_of_Diospyros_malabarica_Kostel_bark_ DB - PRIME DP - Unbound Medicine ER -