Tags

Type your tag names separated by a space and hit enter

Habituation to repeated restraint stress is associated with lack of stress-induced c-fos expression in primary sensory processing areas of the rat brain.
Neuroscience 2006; 138(4):1067-81N

Abstract

Rats repeatedly exposed to restraint show a reduced hypothalamic-pituitary-adrenal axis response upon restraint re-exposure. This hypothalamic-pituitary-adrenal axis response habituation to restraint does not generalize to other novel stressors and is associated with a decrease in stress-induced c-fos expression in a number of stress-reactive brain regions. We examined whether habituation to repeated restraint is also associated with adaptation of immediate early gene expression in brain regions that process and relay primary sensory information. These brain regions may not be expected to show gene expression adaptation to repeated restraint because of their necessary role in experience discrimination. Rats were divided into a repeated restraint group (five 1-hour daily restraint sessions) and an unstressed group (restraint naïve). On the sixth day rats from each group were either killed with no additional stress experience or at 15, 30 or 60 min during restraint. Immediate early gene expression (corticotrophin-releasing hormone heteronuclear RNA, c-fos mRNA, zif268 mRNA) was determined by in situ hybridization. A reduction in stress-induced hypothalamic-pituitary-adrenal axis hormone secretion (plasma corticosterone and adrenocorticotropic hormone) and immediate early gene expression levels in the paraventricular nucleus of the hypothalamus, the lateral septum and the orbital cortex was observed in repeated restraint as compared with restraint naïve animals. This reduction was already evident at 15 min of restraint. Unexpectedly, we also found in repeated restraint rats a reduction in restraint-induced c-fos expression in primary sensory-processing brain areas (primary somatosensory cortex, and ventroposteriomedial and dorsolateral geniculate nuclei of thalamus). The overall levels of hippocampal mineralocorticoid receptor heteronuclear RNA or glucocorticoid receptor mRNA were not decreased by repeated restraint, as may occur in response to severe chronic stress. We propose that repeated restraint leads to a systems-level adaptation whereby re-exposure to restraint elicits a rapid inhibitory modulation of primary sensory processing (i.e. sensory gating), thereby producing a widespread attenuation of the neural response to restraint.

Authors+Show Affiliations

Department of Psychology, Muenzinger Building, Room D244, University of Colorado, UCB 345, Boulder, CO 80309, USA. girotti@colorado.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16431027

Citation

Girotti, M, et al. "Habituation to Repeated Restraint Stress Is Associated With Lack of Stress-induced C-fos Expression in Primary Sensory Processing Areas of the Rat Brain." Neuroscience, vol. 138, no. 4, 2006, pp. 1067-81.
Girotti M, Pace TW, Gaylord RI, et al. Habituation to repeated restraint stress is associated with lack of stress-induced c-fos expression in primary sensory processing areas of the rat brain. Neuroscience. 2006;138(4):1067-81.
Girotti, M., Pace, T. W., Gaylord, R. I., Rubin, B. A., Herman, J. P., & Spencer, R. L. (2006). Habituation to repeated restraint stress is associated with lack of stress-induced c-fos expression in primary sensory processing areas of the rat brain. Neuroscience, 138(4), pp. 1067-81.
Girotti M, et al. Habituation to Repeated Restraint Stress Is Associated With Lack of Stress-induced C-fos Expression in Primary Sensory Processing Areas of the Rat Brain. Neuroscience. 2006;138(4):1067-81. PubMed PMID: 16431027.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Habituation to repeated restraint stress is associated with lack of stress-induced c-fos expression in primary sensory processing areas of the rat brain. AU - Girotti,M, AU - Pace,T W W, AU - Gaylord,R I, AU - Rubin,B A, AU - Herman,J P, AU - Spencer,R L, Y1 - 2006/01/23/ PY - 2005/09/27/received PY - 2005/11/21/revised PY - 2005/12/01/accepted PY - 2006/1/25/pubmed PY - 2006/6/9/medline PY - 2006/1/25/entrez SP - 1067 EP - 81 JF - Neuroscience JO - Neuroscience VL - 138 IS - 4 N2 - Rats repeatedly exposed to restraint show a reduced hypothalamic-pituitary-adrenal axis response upon restraint re-exposure. This hypothalamic-pituitary-adrenal axis response habituation to restraint does not generalize to other novel stressors and is associated with a decrease in stress-induced c-fos expression in a number of stress-reactive brain regions. We examined whether habituation to repeated restraint is also associated with adaptation of immediate early gene expression in brain regions that process and relay primary sensory information. These brain regions may not be expected to show gene expression adaptation to repeated restraint because of their necessary role in experience discrimination. Rats were divided into a repeated restraint group (five 1-hour daily restraint sessions) and an unstressed group (restraint naïve). On the sixth day rats from each group were either killed with no additional stress experience or at 15, 30 or 60 min during restraint. Immediate early gene expression (corticotrophin-releasing hormone heteronuclear RNA, c-fos mRNA, zif268 mRNA) was determined by in situ hybridization. A reduction in stress-induced hypothalamic-pituitary-adrenal axis hormone secretion (plasma corticosterone and adrenocorticotropic hormone) and immediate early gene expression levels in the paraventricular nucleus of the hypothalamus, the lateral septum and the orbital cortex was observed in repeated restraint as compared with restraint naïve animals. This reduction was already evident at 15 min of restraint. Unexpectedly, we also found in repeated restraint rats a reduction in restraint-induced c-fos expression in primary sensory-processing brain areas (primary somatosensory cortex, and ventroposteriomedial and dorsolateral geniculate nuclei of thalamus). The overall levels of hippocampal mineralocorticoid receptor heteronuclear RNA or glucocorticoid receptor mRNA were not decreased by repeated restraint, as may occur in response to severe chronic stress. We propose that repeated restraint leads to a systems-level adaptation whereby re-exposure to restraint elicits a rapid inhibitory modulation of primary sensory processing (i.e. sensory gating), thereby producing a widespread attenuation of the neural response to restraint. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/16431027/Habituation_to_repeated_restraint_stress_is_associated_with_lack_of_stress_induced_c_fos_expression_in_primary_sensory_processing_areas_of_the_rat_brain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(05)01377-1 DB - PRIME DP - Unbound Medicine ER -