Abstract
INTRODUCTION
Malassezia spp. yeasts are opportunistic and newly emergent diseases. One or more species have been isolated in association with dermatological pathology and systemic disease. Their pathological role has not been fully elucidated since Malassezia spp. are common in normal skin flora.
OBJECTIVE
In the current study, a search was undertaken for genetic markers in the Malassezia spp. isolates that correlate with each type of dermatologic lesion.
MATERIALS AND METHODS
A total of 103 strains of Malassezia spp were isolated from patients with pytiriasis versicolor, seborrheic dermatitis, seborrheic dermatitis HIV (+), and atopic dermatitis, as well as from healthy individuals. Eight isolates from CBS Centraalbureau voor Schimmelcultures (Netherlands) were used as controls. Fingerprinting was done using random amplification of polymorphic DNA technique (RAPD) with three primers (OPA2, OPA4, OPA13). The data were analyzed with Diversity Database and SYN-TAX-PC programs.
RESULTS
Intraspecies genetic heterogeneity in M. furfur, M. globosa, M. restricta, M. slooffiae, M. obtusa was observed, whereas M. sympodialis showed the greatest homogeneity.
CONCLUSION
The dermatological disease caused by these different species was not associated with distinctive RAPD fingerprints.
TY - JOUR
T1 - [Genetic polymorphism of Malassezia spp. yeast isolates from individuals with and without dermatological lesions].
AU - Celis,Adriana M,
AU - Cepero de García,María Caridad,
PY - 2006/1/26/pubmed
PY - 2006/4/21/medline
PY - 2006/1/26/entrez
SP - 481
EP - 7
JF - Biomedica : revista del Instituto Nacional de Salud
JO - Biomedica
VL - 25
IS - 4
N2 - INTRODUCTION: Malassezia spp. yeasts are opportunistic and newly emergent diseases. One or more species have been isolated in association with dermatological pathology and systemic disease. Their pathological role has not been fully elucidated since Malassezia spp. are common in normal skin flora. OBJECTIVE: In the current study, a search was undertaken for genetic markers in the Malassezia spp. isolates that correlate with each type of dermatologic lesion. MATERIALS AND METHODS: A total of 103 strains of Malassezia spp were isolated from patients with pytiriasis versicolor, seborrheic dermatitis, seborrheic dermatitis HIV (+), and atopic dermatitis, as well as from healthy individuals. Eight isolates from CBS Centraalbureau voor Schimmelcultures (Netherlands) were used as controls. Fingerprinting was done using random amplification of polymorphic DNA technique (RAPD) with three primers (OPA2, OPA4, OPA13). The data were analyzed with Diversity Database and SYN-TAX-PC programs. RESULTS: Intraspecies genetic heterogeneity in M. furfur, M. globosa, M. restricta, M. slooffiae, M. obtusa was observed, whereas M. sympodialis showed the greatest homogeneity. CONCLUSION: The dermatological disease caused by these different species was not associated with distinctive RAPD fingerprints.
SN - 0120-4157
UR - https://www.unboundmedicine.com/medline/citation/16433174/[Genetic_polymorphism_of_Malassezia_spp__yeast_isolates_from_individuals_with_and_without_dermatological_lesions]_
DB - PRIME
DP - Unbound Medicine
ER -
