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Expression of human Kallikrein 14 (KLK14) in breast cancer is associated with higher tumour grades and positive nodal status.
Br J Cancer 2006; 94(4):540-7BJ

Abstract

Human kallikrein 14 (KLK14) is a steroid hormone-regulated member of the tissue kallikrein family of serine proteases, for which a prognostic and diagnostic value in breast cancer has been suggested. To further characterise the value of KLK14 as a breast tumour marker, we have carefully analysed KLK14 expression in normal breast tissue and breast cancer both on the RNA level by real-time RT-PCR (n = 39), and on the protein level (n = 127) using a KLK14-specific antibody for immunohistochemistry. We correlated KLK14 protein expression data with available clinico-pathological parameters (mean follow-up time was 55 months) including patient prognosis. KLK14 RNA expression as quantified by real-time RT-PCR was significantly more abundant in breast tumours compared to normal breast tissue (P = 0.027), an issue that had not been clarified recently. Concordantly with the RNA data, cytoplasmic KLK14 protein expression was significantly higher in invasive breast carcinomas compared to normal breast tissues (P = 0.003). Furthermore, KLK14 protein expression was associated with higher tumour grade (P = 0.041) and positive nodal status (P = 0.045) but was not significantly associated with shortened disease-free or overall patient survival time in univariate analyses. We conclude that KLK14 is clearly overexpressed in breast cancer in comparison to normal breast tissues and is positively associated with conventional parameters of tumour aggressiveness, but due to a missing association with survival times, the use of KLK14 immunohistochemistry as a prognostic marker in breast cancer is questionable.

Authors+Show Affiliations

Institute of Pathology, Department of Urology, Charité Universitätsmedizin, Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16434994

Citation

Fritzsche, F, et al. "Expression of Human Kallikrein 14 (KLK14) in Breast Cancer Is Associated With Higher Tumour Grades and Positive Nodal Status." British Journal of Cancer, vol. 94, no. 4, 2006, pp. 540-7.
Fritzsche F, Gansukh T, Borgoño CA, et al. Expression of human Kallikrein 14 (KLK14) in breast cancer is associated with higher tumour grades and positive nodal status. Br J Cancer. 2006;94(4):540-7.
Fritzsche, F., Gansukh, T., Borgoño, C. A., Burkhardt, M., Pahl, S., Mayordomo, E., ... Kristiansen, G. (2006). Expression of human Kallikrein 14 (KLK14) in breast cancer is associated with higher tumour grades and positive nodal status. British Journal of Cancer, 94(4), pp. 540-7.
Fritzsche F, et al. Expression of Human Kallikrein 14 (KLK14) in Breast Cancer Is Associated With Higher Tumour Grades and Positive Nodal Status. Br J Cancer. 2006 Feb 27;94(4):540-7. PubMed PMID: 16434994.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of human Kallikrein 14 (KLK14) in breast cancer is associated with higher tumour grades and positive nodal status. AU - Fritzsche,F, AU - Gansukh,T, AU - Borgoño,C A, AU - Burkhardt,M, AU - Pahl,S, AU - Mayordomo,E, AU - Winzer,K-J, AU - Weichert,W, AU - Denkert,C, AU - Jung,K, AU - Stephan,C, AU - Dietel,M, AU - Diamandis,E P, AU - Dahl,E, AU - Kristiansen,G, PY - 2006/1/26/pubmed PY - 2006/3/24/medline PY - 2006/1/26/entrez SP - 540 EP - 7 JF - British journal of cancer JO - Br. J. Cancer VL - 94 IS - 4 N2 - Human kallikrein 14 (KLK14) is a steroid hormone-regulated member of the tissue kallikrein family of serine proteases, for which a prognostic and diagnostic value in breast cancer has been suggested. To further characterise the value of KLK14 as a breast tumour marker, we have carefully analysed KLK14 expression in normal breast tissue and breast cancer both on the RNA level by real-time RT-PCR (n = 39), and on the protein level (n = 127) using a KLK14-specific antibody for immunohistochemistry. We correlated KLK14 protein expression data with available clinico-pathological parameters (mean follow-up time was 55 months) including patient prognosis. KLK14 RNA expression as quantified by real-time RT-PCR was significantly more abundant in breast tumours compared to normal breast tissue (P = 0.027), an issue that had not been clarified recently. Concordantly with the RNA data, cytoplasmic KLK14 protein expression was significantly higher in invasive breast carcinomas compared to normal breast tissues (P = 0.003). Furthermore, KLK14 protein expression was associated with higher tumour grade (P = 0.041) and positive nodal status (P = 0.045) but was not significantly associated with shortened disease-free or overall patient survival time in univariate analyses. We conclude that KLK14 is clearly overexpressed in breast cancer in comparison to normal breast tissues and is positively associated with conventional parameters of tumour aggressiveness, but due to a missing association with survival times, the use of KLK14 immunohistochemistry as a prognostic marker in breast cancer is questionable. SN - 0007-0920 UR - https://www.unboundmedicine.com/medline/citation/16434994/Expression_of_human_Kallikrein_14__KLK14__in_breast_cancer_is_associated_with_higher_tumour_grades_and_positive_nodal_status_ DB - PRIME DP - Unbound Medicine ER -