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The chloroplast genome of Nicotiana sylvestris and Nicotiana tomentosiformis: complete sequencing confirms that the Nicotiana sylvestris progenitor is the maternal genome donor of Nicotiana tabacum.
Mol Genet Genomics 2006; 275(4):367-73MG

Abstract

The tobacco cultivar Nicotiana tabacum is a natural amphidiploid that is thought to be derived from ancestors of Nicotiana sylvestris and Nicotiana tomentosiformis. To compare these chloroplast genomes, DNA was prepared from isolated chloroplasts from green leaves of N. sylvestris and N. tomentosiformis, and subjected to whole-genome shotgun sequencing. The N. sylvestris chloroplast genome comprises of 155,941 bp and shows identical gene organization with that of N. tabacum, except one ORF. Detailed comparison revealed only seven different sites between N. tabacum and N. sylvestris; three in introns, two in spacer regions and two in coding regions. The chloroplast DNA of N. tomentosiformis is 155,745 bp long and possesses also identical gene organization with that of N. tabacum, except four ORFs and one pseudogene. However, 1,194 sites differ between these two species. Compared with N. tabacum, the nucleotide substitution in the inverted repeat was much lower than that in the single-copy region. The present work confirms that the chloroplast genome from N. tabacum was derived from an ancestor of N. sylvestris, and suggests that the rate of nucleotide substitution of the chloroplast genomes from N. tabacum and N. sylvestris is very low.

Authors+Show Affiliations

Graduate School of Natural Sciences, Nagoya City University, 467-8501 Mizuho, Nagoya, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16435119

Citation

Yukawa, M, et al. "The Chloroplast Genome of Nicotiana Sylvestris and Nicotiana Tomentosiformis: Complete Sequencing Confirms That the Nicotiana Sylvestris Progenitor Is the Maternal Genome Donor of Nicotiana Tabacum." Molecular Genetics and Genomics : MGG, vol. 275, no. 4, 2006, pp. 367-73.
Yukawa M, Tsudzuki T, Sugiura M. The chloroplast genome of Nicotiana sylvestris and Nicotiana tomentosiformis: complete sequencing confirms that the Nicotiana sylvestris progenitor is the maternal genome donor of Nicotiana tabacum. Mol Genet Genomics. 2006;275(4):367-73.
Yukawa, M., Tsudzuki, T., & Sugiura, M. (2006). The chloroplast genome of Nicotiana sylvestris and Nicotiana tomentosiformis: complete sequencing confirms that the Nicotiana sylvestris progenitor is the maternal genome donor of Nicotiana tabacum. Molecular Genetics and Genomics : MGG, 275(4), pp. 367-73.
Yukawa M, Tsudzuki T, Sugiura M. The Chloroplast Genome of Nicotiana Sylvestris and Nicotiana Tomentosiformis: Complete Sequencing Confirms That the Nicotiana Sylvestris Progenitor Is the Maternal Genome Donor of Nicotiana Tabacum. Mol Genet Genomics. 2006;275(4):367-73. PubMed PMID: 16435119.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The chloroplast genome of Nicotiana sylvestris and Nicotiana tomentosiformis: complete sequencing confirms that the Nicotiana sylvestris progenitor is the maternal genome donor of Nicotiana tabacum. AU - Yukawa,M, AU - Tsudzuki,T, AU - Sugiura,M, Y1 - 2006/01/25/ PY - 2005/11/07/received PY - 2005/12/10/accepted PY - 2006/1/26/pubmed PY - 2006/6/29/medline PY - 2006/1/26/entrez SP - 367 EP - 73 JF - Molecular genetics and genomics : MGG JO - Mol. Genet. Genomics VL - 275 IS - 4 N2 - The tobacco cultivar Nicotiana tabacum is a natural amphidiploid that is thought to be derived from ancestors of Nicotiana sylvestris and Nicotiana tomentosiformis. To compare these chloroplast genomes, DNA was prepared from isolated chloroplasts from green leaves of N. sylvestris and N. tomentosiformis, and subjected to whole-genome shotgun sequencing. The N. sylvestris chloroplast genome comprises of 155,941 bp and shows identical gene organization with that of N. tabacum, except one ORF. Detailed comparison revealed only seven different sites between N. tabacum and N. sylvestris; three in introns, two in spacer regions and two in coding regions. The chloroplast DNA of N. tomentosiformis is 155,745 bp long and possesses also identical gene organization with that of N. tabacum, except four ORFs and one pseudogene. However, 1,194 sites differ between these two species. Compared with N. tabacum, the nucleotide substitution in the inverted repeat was much lower than that in the single-copy region. The present work confirms that the chloroplast genome from N. tabacum was derived from an ancestor of N. sylvestris, and suggests that the rate of nucleotide substitution of the chloroplast genomes from N. tabacum and N. sylvestris is very low. SN - 1617-4615 UR - https://www.unboundmedicine.com/medline/citation/16435119/The_chloroplast_genome_of_Nicotiana_sylvestris_and_Nicotiana_tomentosiformis:_complete_sequencing_confirms_that_the_Nicotiana_sylvestris_progenitor_is_the_maternal_genome_donor_of_Nicotiana_tabacum_ L2 - https://dx.doi.org/10.1007/s00438-005-0092-6 DB - PRIME DP - Unbound Medicine ER -