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Determination of drugs of abuse and their metabolites in human plasma by liquid chromatography-mass spectrometry. An application to 156 road fatalities.

Abstract

A method, using 0.2 ml of plasma, was designed for the simultaneous determination of morphine, 6-monoacetylmorphine, amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB, benzoylecgonine and cocaine. The drugs were analysed by LC-MS, after solid phase extraction in the presence of the deuterated analogues. Reversed phase separation on an Atlantis dC18 column was achieved in 10 min, under gradient conditions. The method was full validated, including linearity (2-250 ng/ml, r2>0.99), recovery (>50%), within-day and between-day precision and accuracy (CV and bias <15%), limit of detection (0.5 and 1 ng/ml) and quantitation (2 ng/ml), relative ion intensities and no matrix effect was observed. The procedure showed to be sensitive and specific, and was applied to 156 real cases from road fatalities (7.1% cases positive to cocaine and 0.6% to designer drugs).

Authors+Show Affiliations

Forensic Toxicology Service, Institute of Legal Medicine, University of Santiago de Compostela, Spain, C/San Francisco s/n, 15782 Santiago de Compostela, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

16436334

Citation

Concheiro, Marta, et al. "Determination of Drugs of Abuse and Their Metabolites in Human Plasma By Liquid Chromatography-mass Spectrometry. an Application to 156 Road Fatalities." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 832, no. 1, 2006, pp. 81-9.
Concheiro M, de Castro A, Quintela O, et al. Determination of drugs of abuse and their metabolites in human plasma by liquid chromatography-mass spectrometry. An application to 156 road fatalities. J Chromatogr B Analyt Technol Biomed Life Sci. 2006;832(1):81-9.
Concheiro, M., de Castro, A., Quintela, O., López-Rivadulla, M., & Cruz, A. (2006). Determination of drugs of abuse and their metabolites in human plasma by liquid chromatography-mass spectrometry. An application to 156 road fatalities. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 832(1), 81-9.
Concheiro M, et al. Determination of Drugs of Abuse and Their Metabolites in Human Plasma By Liquid Chromatography-mass Spectrometry. an Application to 156 Road Fatalities. J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Feb 17;832(1):81-9. PubMed PMID: 16436334.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of drugs of abuse and their metabolites in human plasma by liquid chromatography-mass spectrometry. An application to 156 road fatalities. AU - Concheiro,Marta, AU - de Castro,Ana, AU - Quintela,Oscar, AU - López-Rivadulla,Manuel, AU - Cruz,Angelines, Y1 - 2006/01/24/ PY - 2005/11/10/received PY - 2005/12/21/revised PY - 2005/12/22/accepted PY - 2006/1/27/pubmed PY - 2006/4/25/medline PY - 2006/1/27/entrez SP - 81 EP - 9 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 832 IS - 1 N2 - A method, using 0.2 ml of plasma, was designed for the simultaneous determination of morphine, 6-monoacetylmorphine, amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB, benzoylecgonine and cocaine. The drugs were analysed by LC-MS, after solid phase extraction in the presence of the deuterated analogues. Reversed phase separation on an Atlantis dC18 column was achieved in 10 min, under gradient conditions. The method was full validated, including linearity (2-250 ng/ml, r2>0.99), recovery (>50%), within-day and between-day precision and accuracy (CV and bias <15%), limit of detection (0.5 and 1 ng/ml) and quantitation (2 ng/ml), relative ion intensities and no matrix effect was observed. The procedure showed to be sensitive and specific, and was applied to 156 real cases from road fatalities (7.1% cases positive to cocaine and 0.6% to designer drugs). SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16436334/Determination_of_drugs_of_abuse_and_their_metabolites_in_human_plasma_by_liquid_chromatography_mass_spectrometry__An_application_to_156_road_fatalities_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(06)00005-5 DB - PRIME DP - Unbound Medicine ER -