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Effects of arginine supplementation on splenocyte cytokine mRNA expression in rats with gut-derived sepsis.
World J Gastroenterol. 2005 Dec 07; 11(45):7091-6.WJ

Abstract

AIM

To investigate the effects of arginine (Arg)-enriched diets before sepsis and/or Arg-containing total parenteral nutrition (TPN) after sepsis or both on cytokine mRNA expression levels in splenocytes of rats with gut-derived sepsis.

METHODS

Rats were assigned to four experimental groups. Groups 1 and 2 were fed with a semipurified diet, while groups 3 and 4 had part of the casein replaced by Arg which provided 2% of the total calories. After the rats were fed with these diets for 10 d, sepsis was induced by cecal ligation and puncture (CLP), at the same time an internal jugular vein was cannulated. All rats were maintained on TPN for 3 d. Groups 1 and 3 were infused with conventional TPN, while groups 2 and 4 were supplemented with Arg which provided 2% of the total calories in the TPN solution. All rats were killed 3 d after CLP to examine their splenocyte subpopulation distribution and cytokine expression levels.

RESULTS

Plasma interleukin (IL)-2, IL-4, tumor necrosis factor-alpha (TNF-alpha) and interferon (IFN-gamma) were not detectable 3 d after CLP. There were no differences in the distributions of CD45Ra(+), CD3(+), CD4(+), and CD8(+) cells in whole blood and splenocytes among the four groups. The splenocyte IL-2 mRNA expression in the Arg-supplemented groups was significantly higher than that in group 1. IL-4 mRNA expression in groups 3 and 4 was significantly higher than that in groups 1 and 2. The mRNA expression of IL-10 and IFN-gamma was significantly higher in group 4 than in the other three groups. There was no difference in TNF-alpha mRNA expression among the four groups.

CONCLUSION

The influence of Arg on the whole blood and splenic lymphocyte subpopulation distribution is not obvious. However, Arg administration, especially before and after CLP, significantly enhances the mRNA expression levels of Th1 and Th2 cytokines in the spleen of rats with gut-derived sepsis.

Authors+Show Affiliations

School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei, Taiwan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16437653

Citation

Shang, Huey-Fang, et al. "Effects of Arginine Supplementation On Splenocyte Cytokine mRNA Expression in Rats With Gut-derived Sepsis." World Journal of Gastroenterology, vol. 11, no. 45, 2005, pp. 7091-6.
Shang HF, Hsu CS, Yeh CL, et al. Effects of arginine supplementation on splenocyte cytokine mRNA expression in rats with gut-derived sepsis. World J Gastroenterol. 2005;11(45):7091-6.
Shang, H. F., Hsu, C. S., Yeh, C. L., Pai, M. H., & Yeh, S. L. (2005). Effects of arginine supplementation on splenocyte cytokine mRNA expression in rats with gut-derived sepsis. World Journal of Gastroenterology, 11(45), 7091-6.
Shang HF, et al. Effects of Arginine Supplementation On Splenocyte Cytokine mRNA Expression in Rats With Gut-derived Sepsis. World J Gastroenterol. 2005 Dec 7;11(45):7091-6. PubMed PMID: 16437653.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of arginine supplementation on splenocyte cytokine mRNA expression in rats with gut-derived sepsis. AU - Shang,Huey-Fang, AU - Hsu,Chun-Sen, AU - Yeh,Chiu-Li, AU - Pai,Man-Hui, AU - Yeh,Sung-Ling, PY - 2006/1/27/pubmed PY - 2006/3/21/medline PY - 2006/1/27/entrez SP - 7091 EP - 6 JF - World journal of gastroenterology JO - World J Gastroenterol VL - 11 IS - 45 N2 - AIM: To investigate the effects of arginine (Arg)-enriched diets before sepsis and/or Arg-containing total parenteral nutrition (TPN) after sepsis or both on cytokine mRNA expression levels in splenocytes of rats with gut-derived sepsis. METHODS: Rats were assigned to four experimental groups. Groups 1 and 2 were fed with a semipurified diet, while groups 3 and 4 had part of the casein replaced by Arg which provided 2% of the total calories. After the rats were fed with these diets for 10 d, sepsis was induced by cecal ligation and puncture (CLP), at the same time an internal jugular vein was cannulated. All rats were maintained on TPN for 3 d. Groups 1 and 3 were infused with conventional TPN, while groups 2 and 4 were supplemented with Arg which provided 2% of the total calories in the TPN solution. All rats were killed 3 d after CLP to examine their splenocyte subpopulation distribution and cytokine expression levels. RESULTS: Plasma interleukin (IL)-2, IL-4, tumor necrosis factor-alpha (TNF-alpha) and interferon (IFN-gamma) were not detectable 3 d after CLP. There were no differences in the distributions of CD45Ra(+), CD3(+), CD4(+), and CD8(+) cells in whole blood and splenocytes among the four groups. The splenocyte IL-2 mRNA expression in the Arg-supplemented groups was significantly higher than that in group 1. IL-4 mRNA expression in groups 3 and 4 was significantly higher than that in groups 1 and 2. The mRNA expression of IL-10 and IFN-gamma was significantly higher in group 4 than in the other three groups. There was no difference in TNF-alpha mRNA expression among the four groups. CONCLUSION: The influence of Arg on the whole blood and splenic lymphocyte subpopulation distribution is not obvious. However, Arg administration, especially before and after CLP, significantly enhances the mRNA expression levels of Th1 and Th2 cytokines in the spleen of rats with gut-derived sepsis. SN - 1007-9327 UR - https://www.unboundmedicine.com/medline/citation/16437653/Effects_of_arginine_supplementation_on_splenocyte_cytokine_mRNA_expression_in_rats_with_gut_derived_sepsis_ L2 - https://www.wjgnet.com/1007-9327/full/v11/i45/7091.htm DB - PRIME DP - Unbound Medicine ER -