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Effect and mechanism of total saponin of Dioscorea on animal experimental hyperuricemia.
Am J Chin Med. 2006; 34(1):77-85.AJ

Abstract

In this study, we investigated the effects and mechanisms of Total Saponin of Dioscorea (TSD) on animal experimental hyperuricemia. Mouse and rat hyperuricemic models were made by orally administering yeast extract paste once a day (30 and 20 g/kg, respectively), for 7 days. Yeast would disturb normal purine metabolism by increasing xanthine oxidase (XOD) activity and generating large quantities of uric acid. This model is similar to human hyperuricemia, which is induced by high-protein diets, due to a purine and nucleic acid metabolic disturbance. Another mouse hyperuricemia model was generated by intraperitoneal injection once with uric acid 250 mg/kg or potassium oxonate 300 mg/kg. Potassium oxonate, a urate oxidase inhibitor, can raise the serum uric acid level by inhibiting the decomposition of uric acid. Likewise, injecting uric acid can also increase serum uric acid concentration. The concentration of uric acid in serum or urine was detected by the phosphotungstic acid method, and the activity of XOD was assayed by a test kit. The results showed that TSD (240, 120 and 60 mg/kg, ig) could significantly lower the level of serum uric acid in hyperuricemic mice. TSD (120 and 60 mg/kg, ig) could also lower the level of serum uric acid in hyperuricemic rats, reduce the activity of XOD in the serum and liver of hyperuricemic rats, and increase the level of urine uric acid concentration as well as 24-hour total uric acid excretion. In conclusion, TSD possesses a potent anti-hyperuricemic effect on hyperuricemic animals, and the mechanism may be relevant in accelerating the excretion and decreasing the production of uric acid.

Authors+Show Affiliations

Institute of Clinical Pharmacology, Anhui Medical University, Hefei, China 230032, China. chguangl@163.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16437741

Citation

Chen, Guang-Liang, et al. "Effect and Mechanism of Total Saponin of Dioscorea On Animal Experimental Hyperuricemia." The American Journal of Chinese Medicine, vol. 34, no. 1, 2006, pp. 77-85.
Chen GL, Wei W, Xu SY. Effect and mechanism of total saponin of Dioscorea on animal experimental hyperuricemia. Am J Chin Med. 2006;34(1):77-85.
Chen, G. L., Wei, W., & Xu, S. Y. (2006). Effect and mechanism of total saponin of Dioscorea on animal experimental hyperuricemia. The American Journal of Chinese Medicine, 34(1), 77-85.
Chen GL, Wei W, Xu SY. Effect and Mechanism of Total Saponin of Dioscorea On Animal Experimental Hyperuricemia. Am J Chin Med. 2006;34(1):77-85. PubMed PMID: 16437741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect and mechanism of total saponin of Dioscorea on animal experimental hyperuricemia. AU - Chen,Guang-Liang, AU - Wei,Wei, AU - Xu,Shu-Yun, PY - 2006/1/27/pubmed PY - 2006/9/1/medline PY - 2006/1/27/entrez SP - 77 EP - 85 JF - The American journal of Chinese medicine JO - Am. J. Chin. Med. VL - 34 IS - 1 N2 - In this study, we investigated the effects and mechanisms of Total Saponin of Dioscorea (TSD) on animal experimental hyperuricemia. Mouse and rat hyperuricemic models were made by orally administering yeast extract paste once a day (30 and 20 g/kg, respectively), for 7 days. Yeast would disturb normal purine metabolism by increasing xanthine oxidase (XOD) activity and generating large quantities of uric acid. This model is similar to human hyperuricemia, which is induced by high-protein diets, due to a purine and nucleic acid metabolic disturbance. Another mouse hyperuricemia model was generated by intraperitoneal injection once with uric acid 250 mg/kg or potassium oxonate 300 mg/kg. Potassium oxonate, a urate oxidase inhibitor, can raise the serum uric acid level by inhibiting the decomposition of uric acid. Likewise, injecting uric acid can also increase serum uric acid concentration. The concentration of uric acid in serum or urine was detected by the phosphotungstic acid method, and the activity of XOD was assayed by a test kit. The results showed that TSD (240, 120 and 60 mg/kg, ig) could significantly lower the level of serum uric acid in hyperuricemic mice. TSD (120 and 60 mg/kg, ig) could also lower the level of serum uric acid in hyperuricemic rats, reduce the activity of XOD in the serum and liver of hyperuricemic rats, and increase the level of urine uric acid concentration as well as 24-hour total uric acid excretion. In conclusion, TSD possesses a potent anti-hyperuricemic effect on hyperuricemic animals, and the mechanism may be relevant in accelerating the excretion and decreasing the production of uric acid. SN - 0192-415X UR - https://www.unboundmedicine.com/medline/citation/16437741/Effect_and_mechanism_of_total_saponin_of_Dioscorea_on_animal_experimental_hyperuricemia_ L2 - https://www.worldscientific.com/doi/full/10.1142/S0192415X06003655?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -