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Quantitative detection of Streptococcus mutans in the dental plaque of Japanese preschool children by real-time PCR.
Lett Appl Microbiol. 2006 Feb; 42(2):127-31.LA

Abstract

AIMS

To detect quantitatively the total bacteria and Streptococcus mutans in dental plaque by real-time PCR with prbac, Sm and GTF-B primers, and to compare their presence with the prevalence of dental caries in Japanese preschool children.

METHODS AND RESULTS

Human dental plaque samples were collected from the labial surfaces of the upper primary central incisors of 107 children. The dental status was recorded as dft by WHO caries diagnostic criteria. Positive dt and dft scores by the Sm or GTF-B primer were significantly higher than negative scores (P < 0.01). The proportions of Strep. mutans to the total bacteria from sound, and sound and/or filled upper primary incisors were significantly lower than those from decayed or filled, and decayed incisors, respectively (P < 0.01).

CONCLUSIONS

The ratios of Strep. mutans to total bacteria in plaque detected by real-time PCR with Sm and GTF-B primers were closely associated with the prevalence of dental caries in Japanese preschool children.

SIGNIFICANCE AND IMPACT OF THE STUDY

These assays may be useful for the assessment of an individual's risk of dental caries.

Authors+Show Affiliations

Department of Oral Health Science, Division of Pediatric Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Japan. arshadrubina@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16441376

Citation

Hata, S, et al. "Quantitative Detection of Streptococcus Mutans in the Dental Plaque of Japanese Preschool Children By Real-time PCR." Letters in Applied Microbiology, vol. 42, no. 2, 2006, pp. 127-31.
Hata S, Hata H, Miyasawa-Hori H, et al. Quantitative detection of Streptococcus mutans in the dental plaque of Japanese preschool children by real-time PCR. Lett Appl Microbiol. 2006;42(2):127-31.
Hata, S., Hata, H., Miyasawa-Hori, H., Kudo, A., & Mayanagi, H. (2006). Quantitative detection of Streptococcus mutans in the dental plaque of Japanese preschool children by real-time PCR. Letters in Applied Microbiology, 42(2), 127-31.
Hata S, et al. Quantitative Detection of Streptococcus Mutans in the Dental Plaque of Japanese Preschool Children By Real-time PCR. Lett Appl Microbiol. 2006;42(2):127-31. PubMed PMID: 16441376.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative detection of Streptococcus mutans in the dental plaque of Japanese preschool children by real-time PCR. AU - Hata,S, AU - Hata,H, AU - Miyasawa-Hori,H, AU - Kudo,A, AU - Mayanagi,H, PY - 2006/1/31/pubmed PY - 2006/5/26/medline PY - 2006/1/31/entrez SP - 127 EP - 31 JF - Letters in applied microbiology JO - Lett Appl Microbiol VL - 42 IS - 2 N2 - AIMS: To detect quantitatively the total bacteria and Streptococcus mutans in dental plaque by real-time PCR with prbac, Sm and GTF-B primers, and to compare their presence with the prevalence of dental caries in Japanese preschool children. METHODS AND RESULTS: Human dental plaque samples were collected from the labial surfaces of the upper primary central incisors of 107 children. The dental status was recorded as dft by WHO caries diagnostic criteria. Positive dt and dft scores by the Sm or GTF-B primer were significantly higher than negative scores (P < 0.01). The proportions of Strep. mutans to the total bacteria from sound, and sound and/or filled upper primary incisors were significantly lower than those from decayed or filled, and decayed incisors, respectively (P < 0.01). CONCLUSIONS: The ratios of Strep. mutans to total bacteria in plaque detected by real-time PCR with Sm and GTF-B primers were closely associated with the prevalence of dental caries in Japanese preschool children. SIGNIFICANCE AND IMPACT OF THE STUDY: These assays may be useful for the assessment of an individual's risk of dental caries. SN - 0266-8254 UR - https://www.unboundmedicine.com/medline/citation/16441376/Quantitative_detection_of_Streptococcus_mutans_in_the_dental_plaque_of_Japanese_preschool_children_by_real_time_PCR_ DB - PRIME DP - Unbound Medicine ER -