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Melatonin attenuates diabetes-induced oxidative stress in rabbits.
J Pineal Res. 2006 Mar; 40(2):168-76.JP

Abstract

Oxidative stress is considered to be the main cause of diabetic complications. As the role of antioxidants in diabetes therapy is still underestimated, the aim of the present investigation was to study the antioxidative action of melatonin in comparison with N-acetylcysteine (NAC) under diabetic conditions. Alloxan-diabetic rabbits were treated daily with either melatonin (1 mg/kg, i.p.), NAC (10 mg/kg, i.p.) or saline. Blood glutathione redox state and serum hydroxyl free radicals (HFR), creatinine and urea levels were monitored. After 3 wk of treatment animals were killed and HFR content, reduced glutathione/oxidized glutathione (GSH/GSSG) ratio as well as the activities of glutathione reductase, glutathione peroxidase and gamma-glutamylcysteine synthetase were estimated in both liver and kidney cortex. Diabetes evoked a several-fold increase in HFR levels accompanied by a significant decline in GSH/GSSG ratio in serum and the examined organs. In contrast to NAC, melatonin (at 1/10 the dose of NAC) attenuated diabetes-induced alterations in glutathione redox state and HFR levels, normalized creatinine concentration and diminished urea content in serum. Moreover, the indole resulted in an increase in glutathione reductase activity in both studied organs and in a rise in glutathione peroxidase and gamma-glutamylcysteine synthetase activities in the liver. In contrast to NAC, melatonin seems to be beneficial for diabetes therapy because of its potent antioxidative and nephroprotective action. The indole-induced increase in the activities of the enzymes of glutathione metabolism might be of importance for antioxidative action of melatonin under diabetic conditions.

Authors+Show Affiliations

Department of Metabolic Regulation, Institute of Biochemistry, Warsaw University, Warsaw, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16441554

Citation

Winiarska, Katarzyna, et al. "Melatonin Attenuates Diabetes-induced Oxidative Stress in Rabbits." Journal of Pineal Research, vol. 40, no. 2, 2006, pp. 168-76.
Winiarska K, Fraczyk T, Malinska D, et al. Melatonin attenuates diabetes-induced oxidative stress in rabbits. J Pineal Res. 2006;40(2):168-76.
Winiarska, K., Fraczyk, T., Malinska, D., Drozak, J., & Bryla, J. (2006). Melatonin attenuates diabetes-induced oxidative stress in rabbits. Journal of Pineal Research, 40(2), 168-76.
Winiarska K, et al. Melatonin Attenuates Diabetes-induced Oxidative Stress in Rabbits. J Pineal Res. 2006;40(2):168-76. PubMed PMID: 16441554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Melatonin attenuates diabetes-induced oxidative stress in rabbits. AU - Winiarska,Katarzyna, AU - Fraczyk,Tomasz, AU - Malinska,Dominika, AU - Drozak,Jakub, AU - Bryla,Jadwiga, PY - 2006/1/31/pubmed PY - 2006/4/14/medline PY - 2006/1/31/entrez SP - 168 EP - 76 JF - Journal of pineal research JO - J Pineal Res VL - 40 IS - 2 N2 - Oxidative stress is considered to be the main cause of diabetic complications. As the role of antioxidants in diabetes therapy is still underestimated, the aim of the present investigation was to study the antioxidative action of melatonin in comparison with N-acetylcysteine (NAC) under diabetic conditions. Alloxan-diabetic rabbits were treated daily with either melatonin (1 mg/kg, i.p.), NAC (10 mg/kg, i.p.) or saline. Blood glutathione redox state and serum hydroxyl free radicals (HFR), creatinine and urea levels were monitored. After 3 wk of treatment animals were killed and HFR content, reduced glutathione/oxidized glutathione (GSH/GSSG) ratio as well as the activities of glutathione reductase, glutathione peroxidase and gamma-glutamylcysteine synthetase were estimated in both liver and kidney cortex. Diabetes evoked a several-fold increase in HFR levels accompanied by a significant decline in GSH/GSSG ratio in serum and the examined organs. In contrast to NAC, melatonin (at 1/10 the dose of NAC) attenuated diabetes-induced alterations in glutathione redox state and HFR levels, normalized creatinine concentration and diminished urea content in serum. Moreover, the indole resulted in an increase in glutathione reductase activity in both studied organs and in a rise in glutathione peroxidase and gamma-glutamylcysteine synthetase activities in the liver. In contrast to NAC, melatonin seems to be beneficial for diabetes therapy because of its potent antioxidative and nephroprotective action. The indole-induced increase in the activities of the enzymes of glutathione metabolism might be of importance for antioxidative action of melatonin under diabetic conditions. SN - 0742-3098 UR - https://www.unboundmedicine.com/medline/citation/16441554/Melatonin_attenuates_diabetes_induced_oxidative_stress_in_rabbits_ DB - PRIME DP - Unbound Medicine ER -