Tags

Type your tag names separated by a space and hit enter

Antihyperalgesic effects of local injections of anandamide, ibuprofen, rofecoxib and their combinations in a model of neuropathic pain.
Neuropharmacology. 2006 Jun; 50(7):814-23.N

Abstract

Nonsteroidal anti-inflammatory drugs (NSAIDs) inhibit fatty acid amidohydrolase (FAAH), the enzyme responsible for the metabolism of anandamide, an endocannabinoid. The analgesic interactions between anandamide (0.01 microg), ibuprofen (0.1 microg) and rofecoxib (0.1 microg) or their combinations administered locally in the hind paw of neuropathic rats were investigated together with the effects of specific antagonists for the cannabinoid CB(1) (AM251; 80 microg) and CB(2) (AM630; 25 microg) receptors. Mechanical allodynia and thermal hyperalgesia were evaluated in 108 Wistar rats allocated to: (1-4) NaCl 0.9%; anandamide; ibuprofen; rofecoxib; (5-6) anandamide+ibuprofen or rofecoxib; (7-8) AM251 or AM630; (9-10) anandamide+AM251 or AM630; (11-12) ibuprofen+AM251 or AM630; (13-14) rofecoxib+AM251 or AM630; (15-16) anandamide+ibuprofen+AM251 or AM630; (17-18) anandamide+rofecoxib+AM251 or AM630. Drugs were given subcutaneously in the hind paw 15min before pain tests. Anandamide, ibuprofen, rofecoxib and their combinations significantly decreased mechanical allodynia and thermal hyperalgesia with an ED(50) of 1.6+/-0.68ng and 1.1+/-1.09 ng for anandamide, respectively. The effects of NSAIDs were not antagonized by AM251 or AM630 but those of anandamide were inhibited by AM251 but not by AM630. In conclusion, locally injected anandamide, ibuprofen, rofecoxib and their combinations decreased pain behavior in neuropathic animals. Local use of endocannabinoids to treat neuropathic pain may be an interesting way to treat this condition without having the deleterious central effects of systemic cannabinoids.

Authors+Show Affiliations

Department of Pharmacology, Faculty of Medicine, Université de Montréal - CHUM, 3840 rue St-Urbain, Montréal, H2W 1T8 Québec, Canada.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16442133

Citation

Guindon, Josée, and Pierre Beaulieu. "Antihyperalgesic Effects of Local Injections of Anandamide, Ibuprofen, Rofecoxib and Their Combinations in a Model of Neuropathic Pain." Neuropharmacology, vol. 50, no. 7, 2006, pp. 814-23.
Guindon J, Beaulieu P. Antihyperalgesic effects of local injections of anandamide, ibuprofen, rofecoxib and their combinations in a model of neuropathic pain. Neuropharmacology. 2006;50(7):814-23.
Guindon, J., & Beaulieu, P. (2006). Antihyperalgesic effects of local injections of anandamide, ibuprofen, rofecoxib and their combinations in a model of neuropathic pain. Neuropharmacology, 50(7), 814-23.
Guindon J, Beaulieu P. Antihyperalgesic Effects of Local Injections of Anandamide, Ibuprofen, Rofecoxib and Their Combinations in a Model of Neuropathic Pain. Neuropharmacology. 2006;50(7):814-23. PubMed PMID: 16442133.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antihyperalgesic effects of local injections of anandamide, ibuprofen, rofecoxib and their combinations in a model of neuropathic pain. AU - Guindon,Josée, AU - Beaulieu,Pierre, Y1 - 2006/01/24/ PY - 2005/07/03/received PY - 2005/11/18/revised PY - 2005/12/06/accepted PY - 2006/1/31/pubmed PY - 2006/10/7/medline PY - 2006/1/31/entrez SP - 814 EP - 23 JF - Neuropharmacology JO - Neuropharmacology VL - 50 IS - 7 N2 - Nonsteroidal anti-inflammatory drugs (NSAIDs) inhibit fatty acid amidohydrolase (FAAH), the enzyme responsible for the metabolism of anandamide, an endocannabinoid. The analgesic interactions between anandamide (0.01 microg), ibuprofen (0.1 microg) and rofecoxib (0.1 microg) or their combinations administered locally in the hind paw of neuropathic rats were investigated together with the effects of specific antagonists for the cannabinoid CB(1) (AM251; 80 microg) and CB(2) (AM630; 25 microg) receptors. Mechanical allodynia and thermal hyperalgesia were evaluated in 108 Wistar rats allocated to: (1-4) NaCl 0.9%; anandamide; ibuprofen; rofecoxib; (5-6) anandamide+ibuprofen or rofecoxib; (7-8) AM251 or AM630; (9-10) anandamide+AM251 or AM630; (11-12) ibuprofen+AM251 or AM630; (13-14) rofecoxib+AM251 or AM630; (15-16) anandamide+ibuprofen+AM251 or AM630; (17-18) anandamide+rofecoxib+AM251 or AM630. Drugs were given subcutaneously in the hind paw 15min before pain tests. Anandamide, ibuprofen, rofecoxib and their combinations significantly decreased mechanical allodynia and thermal hyperalgesia with an ED(50) of 1.6+/-0.68ng and 1.1+/-1.09 ng for anandamide, respectively. The effects of NSAIDs were not antagonized by AM251 or AM630 but those of anandamide were inhibited by AM251 but not by AM630. In conclusion, locally injected anandamide, ibuprofen, rofecoxib and their combinations decreased pain behavior in neuropathic animals. Local use of endocannabinoids to treat neuropathic pain may be an interesting way to treat this condition without having the deleterious central effects of systemic cannabinoids. SN - 0028-3908 UR - https://www.unboundmedicine.com/medline/citation/16442133/Antihyperalgesic_effects_of_local_injections_of_anandamide_ibuprofen_rofecoxib_and_their_combinations_in_a_model_of_neuropathic_pain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0028-3908(05)00413-2 DB - PRIME DP - Unbound Medicine ER -