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Biomonitoring of the genotoxic potential of aqueous extracts of soils and bottom ash resulting from municipal solid waste incineration, using the comet and micronucleus tests on amphibian (Xenopus laevis) larvae and bacterial assays (Mutatox and Ames tests).
Sci Total Environ. 2006 Feb 15; 355(1-3):232-46.ST

Abstract

The management of contaminated soils and wastes is a matter of considerable human concern. The present study evaluates the genotoxic potential of aqueous extracts of two soils (leachates) and of bottom ash resulting from municipal solid waste incineration (MSWIBA percolate), using amphibian larvae (Xenopus laevis). Soil A was contaminated by residues of solvents and metals and Soil B by polycyclic aromatic hydrocarbons and metals. MSWIBA was predominantly contaminated by metals. Two genotoxic endpoints were analysed in circulating erythrocytes taken from larvae: clastogenic and/or aneugenic effects (micronucleus induction) after 12 days of exposure and DNA-strand-breaking potency (comet assay) after 1 and 12 days of exposure. In addition, in vitro bacterial assays (Mutatox and Ames tests) were carried out and the results were compared with those of the amphibian test. Physicochemical analyses were also taken into account. Results obtained with the amphibians established the genotoxicity of the aqueous extracts and the comet assay revealed that they were genotoxic from the first day of exposure. The latter test could thus be considered as a genotoxicity-screening tool. Although genotoxicity persisted after 12 days' exposure, DNA damage decreased overall between days 1 and 12 in the MSWIBA percolate, in contrast to the soil leachates. Bacterial tests detected genotoxicity only for the leachate of soil A (Mutatox). The results confirm the ecotoxicological relevance of the amphibian model and underscore the importance of bioassays, as a complement to physico-chemical data, for risk evaluation.

Authors+Show Affiliations

Laboratoire d'Ecologie des Hydrosystèmes -LEH, FRE CNRS UPS 2630, Centre Universitaire de formation et de Recherche Jean-François Champollion, Campus d'Albi, place de Verdun, 81012 Albi Cedex 9, France. florence.mouchet@wanadoo.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16442436

Citation

Mouchet, F, et al. "Biomonitoring of the Genotoxic Potential of Aqueous Extracts of Soils and Bottom Ash Resulting From Municipal Solid Waste Incineration, Using the Comet and Micronucleus Tests On Amphibian (Xenopus Laevis) Larvae and Bacterial Assays (Mutatox and Ames Tests)." The Science of the Total Environment, vol. 355, no. 1-3, 2006, pp. 232-46.
Mouchet F, Gauthier L, Mailhes C, et al. Biomonitoring of the genotoxic potential of aqueous extracts of soils and bottom ash resulting from municipal solid waste incineration, using the comet and micronucleus tests on amphibian (Xenopus laevis) larvae and bacterial assays (Mutatox and Ames tests). Sci Total Environ. 2006;355(1-3):232-46.
Mouchet, F., Gauthier, L., Mailhes, C., Jourdain, M. J., Ferrier, V., Triffault, G., & Devaux, A. (2006). Biomonitoring of the genotoxic potential of aqueous extracts of soils and bottom ash resulting from municipal solid waste incineration, using the comet and micronucleus tests on amphibian (Xenopus laevis) larvae and bacterial assays (Mutatox and Ames tests). The Science of the Total Environment, 355(1-3), 232-46.
Mouchet F, et al. Biomonitoring of the Genotoxic Potential of Aqueous Extracts of Soils and Bottom Ash Resulting From Municipal Solid Waste Incineration, Using the Comet and Micronucleus Tests On Amphibian (Xenopus Laevis) Larvae and Bacterial Assays (Mutatox and Ames Tests). Sci Total Environ. 2006 Feb 15;355(1-3):232-46. PubMed PMID: 16442436.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biomonitoring of the genotoxic potential of aqueous extracts of soils and bottom ash resulting from municipal solid waste incineration, using the comet and micronucleus tests on amphibian (Xenopus laevis) larvae and bacterial assays (Mutatox and Ames tests). AU - Mouchet,F, AU - Gauthier,L, AU - Mailhes,C, AU - Jourdain,M J, AU - Ferrier,V, AU - Triffault,G, AU - Devaux,A, Y1 - 2005/04/07/ PY - 2004/07/26/received PY - 2005/02/18/accepted PY - 2006/1/31/pubmed PY - 2006/4/8/medline PY - 2006/1/31/entrez SP - 232 EP - 46 JF - The Science of the total environment JO - Sci Total Environ VL - 355 IS - 1-3 N2 - The management of contaminated soils and wastes is a matter of considerable human concern. The present study evaluates the genotoxic potential of aqueous extracts of two soils (leachates) and of bottom ash resulting from municipal solid waste incineration (MSWIBA percolate), using amphibian larvae (Xenopus laevis). Soil A was contaminated by residues of solvents and metals and Soil B by polycyclic aromatic hydrocarbons and metals. MSWIBA was predominantly contaminated by metals. Two genotoxic endpoints were analysed in circulating erythrocytes taken from larvae: clastogenic and/or aneugenic effects (micronucleus induction) after 12 days of exposure and DNA-strand-breaking potency (comet assay) after 1 and 12 days of exposure. In addition, in vitro bacterial assays (Mutatox and Ames tests) were carried out and the results were compared with those of the amphibian test. Physicochemical analyses were also taken into account. Results obtained with the amphibians established the genotoxicity of the aqueous extracts and the comet assay revealed that they were genotoxic from the first day of exposure. The latter test could thus be considered as a genotoxicity-screening tool. Although genotoxicity persisted after 12 days' exposure, DNA damage decreased overall between days 1 and 12 in the MSWIBA percolate, in contrast to the soil leachates. Bacterial tests detected genotoxicity only for the leachate of soil A (Mutatox). The results confirm the ecotoxicological relevance of the amphibian model and underscore the importance of bioassays, as a complement to physico-chemical data, for risk evaluation. SN - 0048-9697 UR - https://www.unboundmedicine.com/medline/citation/16442436/Biomonitoring_of_the_genotoxic_potential_of_aqueous_extracts_of_soils_and_bottom_ash_resulting_from_municipal_solid_waste_incineration_using_the_comet_and_micronucleus_tests_on_amphibian__Xenopus_laevis__larvae_and_bacterial_assays__Mutatox_and_Ames_tests__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(05)00183-X DB - PRIME DP - Unbound Medicine ER -