TY - JOUR T1 - Identification of polysaccharides from pericarp tissues of litchi (Litchi chinensis Sonn.) fruit in relation to their antioxidant activities. AU - Yang,Bao, AU - Wang,Jinshui, AU - Zhao,Mouming, AU - Liu,Yang, AU - Wang,Wei, AU - Jiang,Yueming, Y1 - 2006/01/26/ PY - 2005/09/05/received PY - 2005/12/17/revised PY - 2006/01/05/accepted PY - 2006/1/31/pubmed PY - 2006/12/14/medline PY - 2006/1/31/entrez SP - 634 EP - 8 JF - Carbohydrate research JO - Carbohydr Res VL - 341 IS - 5 N2 - A large number of polysaccharides are present in the pericarp tissues of harvested litchi fruits. A DEAE Sepharose fast-flow anion-exchange column and a Sephadex G-50 gel-permeation column were used to isolate and purify the major polysaccharides from litchi fruit pericarp tissues. Antioxidant activities of these major polysaccharide components were also evaluated. An aqueous extract of the polysaccharides from litchi fruit pericarp tissues was chromatographed on a DEAE anion-exchange column to yield two fractions. The largest amount of the polysaccharide fraction was subjected to further purification by gel filtration on Sephadex G-50. The purified product was a neutral polysaccharide, with a molecular weight of 14 kDa, comprised mainly of 65.6% mannose, 33.0% galactose and 1.4% arabinose. Analysis by Smith degradation indicated that there were 8.7% of (1-->2)-glycosidic linkages, 83.3% of (1-->3)-glycosidic linkages and 8.0% of (1-->6)-glycosidic linkages in the polysaccharide. Furthermore, different polysaccharide fractions extracted and purified from litchi fruit pericarp tissues exhibited strong antioxidant activities. Among these fractions, the purified polysaccharide had the highest antioxidant activity and should be explored as a novel potential antioxidant. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/16442509/Identification_of_polysaccharides_from_pericarp_tissues_of_litchi__Litchi_chinensis_Sonn___fruit_in_relation_to_their_antioxidant_activities_ DB - PRIME DP - Unbound Medicine ER -