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Alginate microspheres prepared by internal gelation: development and effect on insulin stability.
Int J Pharm. 2006 Mar 27; 311(1-2):1-10.IJ

Abstract

Recombinant human insulin was encapsulated within alginate microspheres by the emulsification/internal gelation technique with the objective of preserving protein stability during encapsulation procedure. The influence of process and formulation parameters was evaluated on the morphology and encapsulation efficiency of insulin. The in vitro release of insulin from microspheres was studied under simulated gastrointestinal conditions and the in vivo activity of protein after processing was assessed by subcutaneous administration of extracted insulin from microspheres to streptozotocin-induced diabetic rats. Microspheres mean diameter, ranging from 21 to 287 microm, decreased with the internal phase ratio, emulsifier concentration, mixer rotational speed and increased with alginate concentration. Insulin encapsulation efficiency, near 75%, was not affected by emulsifier concentration, mixer rotational speed and zinc/insulin hexamer molar ratio but decreased either by increasing internal phase ratio and calcium/alginate mass ratio or by decreasing acid/calcium molar ratio and alginate concentration. A high insulin release, above 75%, was obtained at pH 1.2 and under simulated intestinal pH a complete dissolution of microspheres occurred. Extracted insulin from microspheres decreased hyperglycemia of diabetic rats proving to be bioactive and showing that encapsulation in alginate microspheres using the emulsification/internal gelation is an appropriate method for protein encapsulation.

Authors+Show Affiliations

Laboratório de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade de Coimbra, Rua do Norte, 3000-295 Coimbra, Portugal. catarinasilva@ci.uc.ptNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16442757

Citation

Silva, Catarina M., et al. "Alginate Microspheres Prepared By Internal Gelation: Development and Effect On Insulin Stability." International Journal of Pharmaceutics, vol. 311, no. 1-2, 2006, pp. 1-10.
Silva CM, Ribeiro AJ, Figueiredo IV, et al. Alginate microspheres prepared by internal gelation: development and effect on insulin stability. Int J Pharm. 2006;311(1-2):1-10.
Silva, C. M., Ribeiro, A. J., Figueiredo, I. V., Gonçalves, A. R., & Veiga, F. (2006). Alginate microspheres prepared by internal gelation: development and effect on insulin stability. International Journal of Pharmaceutics, 311(1-2), 1-10.
Silva CM, et al. Alginate Microspheres Prepared By Internal Gelation: Development and Effect On Insulin Stability. Int J Pharm. 2006 Mar 27;311(1-2):1-10. PubMed PMID: 16442757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alginate microspheres prepared by internal gelation: development and effect on insulin stability. AU - Silva,Catarina M, AU - Ribeiro,António J, AU - Figueiredo,Isabel Vitória, AU - Gonçalves,Alexandra Rocha, AU - Veiga,Francisco, Y1 - 2006/01/26/ PY - 2005/07/08/received PY - 2005/10/11/revised PY - 2005/10/15/accepted PY - 2006/1/31/pubmed PY - 2006/10/17/medline PY - 2006/1/31/entrez SP - 1 EP - 10 JF - International journal of pharmaceutics JO - Int J Pharm VL - 311 IS - 1-2 N2 - Recombinant human insulin was encapsulated within alginate microspheres by the emulsification/internal gelation technique with the objective of preserving protein stability during encapsulation procedure. The influence of process and formulation parameters was evaluated on the morphology and encapsulation efficiency of insulin. The in vitro release of insulin from microspheres was studied under simulated gastrointestinal conditions and the in vivo activity of protein after processing was assessed by subcutaneous administration of extracted insulin from microspheres to streptozotocin-induced diabetic rats. Microspheres mean diameter, ranging from 21 to 287 microm, decreased with the internal phase ratio, emulsifier concentration, mixer rotational speed and increased with alginate concentration. Insulin encapsulation efficiency, near 75%, was not affected by emulsifier concentration, mixer rotational speed and zinc/insulin hexamer molar ratio but decreased either by increasing internal phase ratio and calcium/alginate mass ratio or by decreasing acid/calcium molar ratio and alginate concentration. A high insulin release, above 75%, was obtained at pH 1.2 and under simulated intestinal pH a complete dissolution of microspheres occurred. Extracted insulin from microspheres decreased hyperglycemia of diabetic rats proving to be bioactive and showing that encapsulation in alginate microspheres using the emulsification/internal gelation is an appropriate method for protein encapsulation. SN - 0378-5173 UR - https://www.unboundmedicine.com/medline/citation/16442757/Alginate_microspheres_prepared_by_internal_gelation:_development_and_effect_on_insulin_stability_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-5173(05)00857-4 DB - PRIME DP - Unbound Medicine ER -