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Role of cannabinoid receptors in Delta-9-tetrahydrocannabinol suppression of IL-12p40 in mouse bone marrow-derived dendritic cells infected with Legionella pneumophila.
Eur J Pharmacol. 2006 Feb 17; 532(1-2):170-7.EJ

Abstract

Delta-9-tetrahydrocannabinol (THC) injection suppresses serum interleukin-12 (IL-12) levels in Legionella pneumophila-infected mice. Dendritic cells are a major producer of IL-12 and mouse, bone marrow-derived dendritic cell cultures produced high levels of the IL-12p40 following L. pneumophila infection. Treatment with THC suppressed this cytokine response in a concentration-dependent manner and the endocannabinoid, 2-arachidonoyolglycerol, less potently suppressed cytokine production. Dendritic cells expressed mRNA for cannabinoid receptor 1 (CB(1)), cannabinoid CB(2) receptor, and vanilloid receptor 1 (TRPV1) and the addition of the G(i) inhibitor, pertussis toxin, completely attenuated suppression induced by 3 and 6 muM THC but not by 10 muM THC. Furthermore, THC suppression was partially attenuated in dendritic cells from cannabinoid CB(1) receptor and CB(2) receptor knockout mice and in dendritic cells co-treated with THC and cannabinoid receptor antagonists. Cytokine suppression was not attenuated by pretreatment with the TRPV1 antagonist, capsazepine. These results suggest that THC-induced suppression of serum IL-12 is partly due to a suppression of IL-12 production by dendritic cells and that G(i) signaling and cannabinoid receptors, but not TRPV1, are involved in this suppressive effect.

Authors+Show Affiliations

Department of Medical Microbiology and Immunology, MDC 10 University of South Florida College of Medicine, 12901 Bruce B. Downs Blvd., Tampa, FL 33612, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16443217

Citation

Lu, Tangying, et al. "Role of Cannabinoid Receptors in Delta-9-tetrahydrocannabinol Suppression of IL-12p40 in Mouse Bone Marrow-derived Dendritic Cells Infected With Legionella Pneumophila." European Journal of Pharmacology, vol. 532, no. 1-2, 2006, pp. 170-7.
Lu T, Newton C, Perkins I, et al. Role of cannabinoid receptors in Delta-9-tetrahydrocannabinol suppression of IL-12p40 in mouse bone marrow-derived dendritic cells infected with Legionella pneumophila. Eur J Pharmacol. 2006;532(1-2):170-7.
Lu, T., Newton, C., Perkins, I., Friedman, H., & Klein, T. W. (2006). Role of cannabinoid receptors in Delta-9-tetrahydrocannabinol suppression of IL-12p40 in mouse bone marrow-derived dendritic cells infected with Legionella pneumophila. European Journal of Pharmacology, 532(1-2), 170-7.
Lu T, et al. Role of Cannabinoid Receptors in Delta-9-tetrahydrocannabinol Suppression of IL-12p40 in Mouse Bone Marrow-derived Dendritic Cells Infected With Legionella Pneumophila. Eur J Pharmacol. 2006 Feb 17;532(1-2):170-7. PubMed PMID: 16443217.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of cannabinoid receptors in Delta-9-tetrahydrocannabinol suppression of IL-12p40 in mouse bone marrow-derived dendritic cells infected with Legionella pneumophila. AU - Lu,Tangying, AU - Newton,Cathy, AU - Perkins,Izabella, AU - Friedman,Herman, AU - Klein,Thomas W, Y1 - 2006/01/27/ PY - 2005/09/01/received PY - 2005/12/16/revised PY - 2005/12/19/accepted PY - 2006/1/31/pubmed PY - 2006/6/3/medline PY - 2006/1/31/entrez SP - 170 EP - 7 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 532 IS - 1-2 N2 - Delta-9-tetrahydrocannabinol (THC) injection suppresses serum interleukin-12 (IL-12) levels in Legionella pneumophila-infected mice. Dendritic cells are a major producer of IL-12 and mouse, bone marrow-derived dendritic cell cultures produced high levels of the IL-12p40 following L. pneumophila infection. Treatment with THC suppressed this cytokine response in a concentration-dependent manner and the endocannabinoid, 2-arachidonoyolglycerol, less potently suppressed cytokine production. Dendritic cells expressed mRNA for cannabinoid receptor 1 (CB(1)), cannabinoid CB(2) receptor, and vanilloid receptor 1 (TRPV1) and the addition of the G(i) inhibitor, pertussis toxin, completely attenuated suppression induced by 3 and 6 muM THC but not by 10 muM THC. Furthermore, THC suppression was partially attenuated in dendritic cells from cannabinoid CB(1) receptor and CB(2) receptor knockout mice and in dendritic cells co-treated with THC and cannabinoid receptor antagonists. Cytokine suppression was not attenuated by pretreatment with the TRPV1 antagonist, capsazepine. These results suggest that THC-induced suppression of serum IL-12 is partly due to a suppression of IL-12 production by dendritic cells and that G(i) signaling and cannabinoid receptors, but not TRPV1, are involved in this suppressive effect. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/16443217/Role_of_cannabinoid_receptors_in_Delta_9_tetrahydrocannabinol_suppression_of_IL_12p40_in_mouse_bone_marrow_derived_dendritic_cells_infected_with_Legionella_pneumophila_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(05)01351-8 DB - PRIME DP - Unbound Medicine ER -