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Immunohistochemical characterization of glomerular inflammatory cells and expression of adhesion molecules in anti-glomerular basement membrane (anti-GBM) glomerulonephritis induced in WKY rats with monoclonal anti-GBM antibodies of different subclasses.
Pathol Int. 2006 Feb; 56(2):55-61.PI

Abstract

According to previous report, adhesion of CD8-positive cells and macrophages to glomerular endotherial cells through the lymphocyte function-associated antigen-1 (LFA-1)/intercellular adhesion molecule-1 (ICAM-1) pathway is crucial for the initiation and subsequent progression of anti-glomerular basement membrane (anti-GBM) antibody-induced glomerulonephritis (anti-GBM nephritis) in WKY rats. In the present study glomerular inflammatory cell infiltration and LFA-1/ICAM-1 expression were examined in anti-GBM nephritis induced in WKY rats with monoclonal anti-GBM antibodies of different subclasses: IgG1, IgG2a, and IgG2b. The IgG2a and IgG2b subclasses induced significant proteinuria from day 3 as compared with the IgG1 subclass. Glomerular infiltration of macrophages and CD8-positive cells after administration of IgG2a and IgG2b subclass antibodies was significantly elevated compared to that for the IgG1 subclass. The intensity of glomerular ICAM-1 immunostaining by the IgG2a and IgG2b subclass antibodies tended to be stronger than that by the IgG1 subclass. Glomerular LFA-1-positive cell infiltration by the IgG2a and IgG2b subclasses was significantly higher than that of the IgG1 subclass. These results demonstrate that monoclonal antibodies belonging to the IgG2a and IgG2b subclasses strongly induce glomerular infiltration of inflammatory cells and expression of adhesion molecules in rat anti-GBM nephritis.

Authors+Show Affiliations

Division of Pediatrics and Perinatology, Faculty of Medicine, Tottori University, Yonago, Japan. CYY03617@nifty.ne.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16445816

Citation

Kado, Tadashi, et al. "Immunohistochemical Characterization of Glomerular Inflammatory Cells and Expression of Adhesion Molecules in Anti-glomerular Basement Membrane (anti-GBM) Glomerulonephritis Induced in WKY Rats With Monoclonal anti-GBM Antibodies of Different Subclasses." Pathology International, vol. 56, no. 2, 2006, pp. 55-61.
Kado T, Kohda T, Okada S, et al. Immunohistochemical characterization of glomerular inflammatory cells and expression of adhesion molecules in anti-glomerular basement membrane (anti-GBM) glomerulonephritis induced in WKY rats with monoclonal anti-GBM antibodies of different subclasses. Pathol Int. 2006;56(2):55-61.
Kado, T., Kohda, T., Okada, S., Hayashi, A., Utsunomiya, Y., Kanzaki, S., Sado, Y., & Yamamoto, T. (2006). Immunohistochemical characterization of glomerular inflammatory cells and expression of adhesion molecules in anti-glomerular basement membrane (anti-GBM) glomerulonephritis induced in WKY rats with monoclonal anti-GBM antibodies of different subclasses. Pathology International, 56(2), 55-61.
Kado T, et al. Immunohistochemical Characterization of Glomerular Inflammatory Cells and Expression of Adhesion Molecules in Anti-glomerular Basement Membrane (anti-GBM) Glomerulonephritis Induced in WKY Rats With Monoclonal anti-GBM Antibodies of Different Subclasses. Pathol Int. 2006;56(2):55-61. PubMed PMID: 16445816.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunohistochemical characterization of glomerular inflammatory cells and expression of adhesion molecules in anti-glomerular basement membrane (anti-GBM) glomerulonephritis induced in WKY rats with monoclonal anti-GBM antibodies of different subclasses. AU - Kado,Tadashi, AU - Kohda,Takayuki, AU - Okada,Shinichi, AU - Hayashi,Atsushi, AU - Utsunomiya,Yasushi, AU - Kanzaki,Susumu, AU - Sado,Yoshikazu, AU - Yamamoto,Tadashi, PY - 2006/2/1/pubmed PY - 2006/5/10/medline PY - 2006/2/1/entrez SP - 55 EP - 61 JF - Pathology international JO - Pathol Int VL - 56 IS - 2 N2 - According to previous report, adhesion of CD8-positive cells and macrophages to glomerular endotherial cells through the lymphocyte function-associated antigen-1 (LFA-1)/intercellular adhesion molecule-1 (ICAM-1) pathway is crucial for the initiation and subsequent progression of anti-glomerular basement membrane (anti-GBM) antibody-induced glomerulonephritis (anti-GBM nephritis) in WKY rats. In the present study glomerular inflammatory cell infiltration and LFA-1/ICAM-1 expression were examined in anti-GBM nephritis induced in WKY rats with monoclonal anti-GBM antibodies of different subclasses: IgG1, IgG2a, and IgG2b. The IgG2a and IgG2b subclasses induced significant proteinuria from day 3 as compared with the IgG1 subclass. Glomerular infiltration of macrophages and CD8-positive cells after administration of IgG2a and IgG2b subclass antibodies was significantly elevated compared to that for the IgG1 subclass. The intensity of glomerular ICAM-1 immunostaining by the IgG2a and IgG2b subclass antibodies tended to be stronger than that by the IgG1 subclass. Glomerular LFA-1-positive cell infiltration by the IgG2a and IgG2b subclasses was significantly higher than that of the IgG1 subclass. These results demonstrate that monoclonal antibodies belonging to the IgG2a and IgG2b subclasses strongly induce glomerular infiltration of inflammatory cells and expression of adhesion molecules in rat anti-GBM nephritis. SN - 1320-5463 UR - https://www.unboundmedicine.com/medline/citation/16445816/Immunohistochemical_characterization_of_glomerular_inflammatory_cells_and_expression_of_adhesion_molecules_in_anti_glomerular_basement_membrane__anti_GBM__glomerulonephritis_induced_in_WKY_rats_with_monoclonal_anti_GBM_antibodies_of_different_subclasses_ L2 - https://doi.org/10.1111/j.1440-1827.2006.01920.x DB - PRIME DP - Unbound Medicine ER -