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[Frequency of K-ras mutation in biliary and pancreatic tumors].
Rev Med Chil. 2005 Dec; 133(12):1434-40.RM

Abstract

BACKGROUND

The ras gene family (H-ras, N-ras and K-ras) are oncogenes that mutate frequently in human cancer, specially in tumors of the biliary tract and pancreas.

AIM

To determine the frequency of K-ras gene codon 12 mutation in pancreatic and biliary tumors.

MATERIAL AND METHODS

Samples of 35 gallbladder, 15 ampulla of Vater, 10 biliary tract and 9 pancreatic tumors, were analyzed. The tumor tissue was microdissected from paraffin embedded biopsies. The mutation was detected by a combination of polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).

RESULTS

Overall, 46% of samples had K-ras gene mutations. Mutation frequency was 80, 56, 50 and 29% for ampulla of Vater, pancreatic, biliary tract and gallbladder tumors, respectively. When compared with the rest, gallbladder tumors had a significantly lower frequency of the mutation. Median survival for biliary tract tumors was 6 months, compared with 65 months for gallbladder tumors (p <0.05).

CONCLUSIONS

Gallbladder carcinoma had the lower frequency of K-ras mutation, when compared with pancreatic, biliary tract and ampulla of Vater tumors.

Authors+Show Affiliations

Departamento de Anatomía Patológica, Facultad de Medicina, Universidad de la Frontera. jcroa@ufro.clNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article

Language

spa

PubMed ID

16446870

Citation

Roa, Juan Carlos, et al. "[Frequency of K-ras Mutation in Biliary and Pancreatic Tumors]." Revista Medica De Chile, vol. 133, no. 12, 2005, pp. 1434-40.
Roa JC, Anabalón L, Tapia O, et al. [Frequency of K-ras mutation in biliary and pancreatic tumors]. Rev Med Chil. 2005;133(12):1434-40.
Roa, J. C., Anabalón, L., Tapia, O., Melo, A., de Aretxabala, X., & Roa, I. (2005). [Frequency of K-ras mutation in biliary and pancreatic tumors]. Revista Medica De Chile, 133(12), 1434-40.
Roa JC, et al. [Frequency of K-ras Mutation in Biliary and Pancreatic Tumors]. Rev Med Chil. 2005;133(12):1434-40. PubMed PMID: 16446870.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Frequency of K-ras mutation in biliary and pancreatic tumors]. AU - Roa,Juan Carlos, AU - Anabalón,Leonardo, AU - Tapia,Oscar, AU - Melo,Angélica, AU - de Aretxabala,Xabier, AU - Roa,Iván, Y1 - 2006/01/27/ PY - 2006/2/1/pubmed PY - 2006/9/29/medline PY - 2006/2/1/entrez SP - 1434 EP - 40 JF - Revista medica de Chile JO - Rev Med Chil VL - 133 IS - 12 N2 - BACKGROUND: The ras gene family (H-ras, N-ras and K-ras) are oncogenes that mutate frequently in human cancer, specially in tumors of the biliary tract and pancreas. AIM: To determine the frequency of K-ras gene codon 12 mutation in pancreatic and biliary tumors. MATERIAL AND METHODS: Samples of 35 gallbladder, 15 ampulla of Vater, 10 biliary tract and 9 pancreatic tumors, were analyzed. The tumor tissue was microdissected from paraffin embedded biopsies. The mutation was detected by a combination of polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). RESULTS: Overall, 46% of samples had K-ras gene mutations. Mutation frequency was 80, 56, 50 and 29% for ampulla of Vater, pancreatic, biliary tract and gallbladder tumors, respectively. When compared with the rest, gallbladder tumors had a significantly lower frequency of the mutation. Median survival for biliary tract tumors was 6 months, compared with 65 months for gallbladder tumors (p <0.05). CONCLUSIONS: Gallbladder carcinoma had the lower frequency of K-ras mutation, when compared with pancreatic, biliary tract and ampulla of Vater tumors. SN - 0034-9887 UR - https://www.unboundmedicine.com/medline/citation/16446870/[Frequency_of_K_ras_mutation_in_biliary_and_pancreatic_tumors]_ L2 - http://www.scielo.cl/scielo.php?script=sci_arttext&amp;pid=S0034-98872005001200004&amp;lng=en&amp;nrm=iso&amp;tlng=en DB - PRIME DP - Unbound Medicine ER -