Tags

Type your tag names separated by a space and hit enter

Prevention of nonspecific bacterial cell adhesion in immunoassays by use of cranberry juice.
Anal Chem. 2006 Feb 01; 78(3):853-7.AC

Abstract

The ability of Vaccinum macrocarpon, the North American cranberry, to prevent bacterial adhesion has been used to advantage in the prevention of urinary tract infections and has recently been described for the prevention of adhesion of bacteria responsible for oral infections and stomach ulcers. This report documents the ability of cranberry juice to reduce nonspecific adhesion of bacteria to the borosilicate glass microscope slides used in an immunoarray biosensor format. Nonspecific binding of analytes in the array sensor leads to high background signals that cause increased detection limits and false positives. Reduction in background-to-signal ratios can be seen as the juice concentration is increased from 0 to 50% of the sample. This impact cannot be duplicated with grape, orange, apple, or white cranberry juice. Sugar content and pH have been eliminated as the agents in the juice responsible for the anti-adhesive activity.

Authors+Show Affiliations

Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16448060

Citation

Johnson-White, Brandy, et al. "Prevention of Nonspecific Bacterial Cell Adhesion in Immunoassays By Use of Cranberry Juice." Analytical Chemistry, vol. 78, no. 3, 2006, pp. 853-7.
Johnson-White B, Buquo L, Zeinali M, et al. Prevention of nonspecific bacterial cell adhesion in immunoassays by use of cranberry juice. Anal Chem. 2006;78(3):853-7.
Johnson-White, B., Buquo, L., Zeinali, M., & Ligler, F. S. (2006). Prevention of nonspecific bacterial cell adhesion in immunoassays by use of cranberry juice. Analytical Chemistry, 78(3), 853-7.
Johnson-White B, et al. Prevention of Nonspecific Bacterial Cell Adhesion in Immunoassays By Use of Cranberry Juice. Anal Chem. 2006 Feb 1;78(3):853-7. PubMed PMID: 16448060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevention of nonspecific bacterial cell adhesion in immunoassays by use of cranberry juice. AU - Johnson-White,Brandy, AU - Buquo,Lauren, AU - Zeinali,Mazyar, AU - Ligler,Frances S, PY - 2006/2/2/pubmed PY - 2007/4/12/medline PY - 2006/2/2/entrez SP - 853 EP - 7 JF - Analytical chemistry JO - Anal. Chem. VL - 78 IS - 3 N2 - The ability of Vaccinum macrocarpon, the North American cranberry, to prevent bacterial adhesion has been used to advantage in the prevention of urinary tract infections and has recently been described for the prevention of adhesion of bacteria responsible for oral infections and stomach ulcers. This report documents the ability of cranberry juice to reduce nonspecific adhesion of bacteria to the borosilicate glass microscope slides used in an immunoarray biosensor format. Nonspecific binding of analytes in the array sensor leads to high background signals that cause increased detection limits and false positives. Reduction in background-to-signal ratios can be seen as the juice concentration is increased from 0 to 50% of the sample. This impact cannot be duplicated with grape, orange, apple, or white cranberry juice. Sugar content and pH have been eliminated as the agents in the juice responsible for the anti-adhesive activity. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/16448060/Prevention_of_nonspecific_bacterial_cell_adhesion_in_immunoassays_by_use_of_cranberry_juice_ L2 - https://dx.doi.org/10.1021/ac051700v DB - PRIME DP - Unbound Medicine ER -