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Apple procyanidin oligomers absorption in rats after oral administration: analysis of procyanidins in plasma using the porter method and high-performance liquid chromatography/tandem mass spectrometry.
J Agric Food Chem. 2006 Feb 08; 54(3):884-92.JA

Abstract

In this study, we investigated the absorption of apple procyanidins, namely, apple condensed tannins (ACTs), in rats using the Porter method and high-performance liquid chromatography/tandem mass spectrometry. The apple procyanidin concentrations in the rat plasma reached a maximum 2 h after administration and decreased thereafter. To investigate the limits of the absorption of apple procyanidins in the polymerization degree, we administered the procyanidin oligomer fraction, which was separated from ACT using normal-phase chromatography according to the degree of polymerization. Procyanidins from each dimer to pentamer group were detected in the plasma by the Porter method. Moreover, by the study using reconstituted procyanidins, polymeric procyanidins influenced the absorption of procyanidin oligomers. These results suggest that ACTs are absorbed and directly involved in physiological functions in the rats.

Authors+Show Affiliations

Fundamental Research Laboratory, Asahi Breweries, Ltd., I-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, Japan. toshihik.shoji@asahibeer.co.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16448199

Citation

Shoji, Toshihiko, et al. "Apple Procyanidin Oligomers Absorption in Rats After Oral Administration: Analysis of Procyanidins in Plasma Using the Porter Method and High-performance Liquid Chromatography/tandem Mass Spectrometry." Journal of Agricultural and Food Chemistry, vol. 54, no. 3, 2006, pp. 884-92.
Shoji T, Masumoto S, Moriichi N, et al. Apple procyanidin oligomers absorption in rats after oral administration: analysis of procyanidins in plasma using the porter method and high-performance liquid chromatography/tandem mass spectrometry. J Agric Food Chem. 2006;54(3):884-92.
Shoji, T., Masumoto, S., Moriichi, N., Akiyama, H., Kanda, T., Ohtake, Y., & Goda, Y. (2006). Apple procyanidin oligomers absorption in rats after oral administration: analysis of procyanidins in plasma using the porter method and high-performance liquid chromatography/tandem mass spectrometry. Journal of Agricultural and Food Chemistry, 54(3), 884-92.
Shoji T, et al. Apple Procyanidin Oligomers Absorption in Rats After Oral Administration: Analysis of Procyanidins in Plasma Using the Porter Method and High-performance Liquid Chromatography/tandem Mass Spectrometry. J Agric Food Chem. 2006 Feb 8;54(3):884-92. PubMed PMID: 16448199.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apple procyanidin oligomers absorption in rats after oral administration: analysis of procyanidins in plasma using the porter method and high-performance liquid chromatography/tandem mass spectrometry. AU - Shoji,Toshihiko, AU - Masumoto,Saeko, AU - Moriichi,Nina, AU - Akiyama,Hiroshi, AU - Kanda,Tomomasa, AU - Ohtake,Yasuyuki, AU - Goda,Yukihiro, PY - 2006/2/2/pubmed PY - 2006/3/29/medline PY - 2006/2/2/entrez SP - 884 EP - 92 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 54 IS - 3 N2 - In this study, we investigated the absorption of apple procyanidins, namely, apple condensed tannins (ACTs), in rats using the Porter method and high-performance liquid chromatography/tandem mass spectrometry. The apple procyanidin concentrations in the rat plasma reached a maximum 2 h after administration and decreased thereafter. To investigate the limits of the absorption of apple procyanidins in the polymerization degree, we administered the procyanidin oligomer fraction, which was separated from ACT using normal-phase chromatography according to the degree of polymerization. Procyanidins from each dimer to pentamer group were detected in the plasma by the Porter method. Moreover, by the study using reconstituted procyanidins, polymeric procyanidins influenced the absorption of procyanidin oligomers. These results suggest that ACTs are absorbed and directly involved in physiological functions in the rats. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16448199/Apple_procyanidin_oligomers_absorption_in_rats_after_oral_administration:_analysis_of_procyanidins_in_plasma_using_the_porter_method_and_high_performance_liquid_chromatography/tandem_mass_spectrometry_ L2 - https://dx.doi.org/10.1021/jf052260b DB - PRIME DP - Unbound Medicine ER -