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Insulin-like growth factor I and II expression and modulation in amoeboid microglial cells by lipopolysaccharide and retinoic acid.

Abstract

Insulin-like growth factors I and II are known to regulate the development of the CNS. We examined the developmental changes in insulin-like growth factor I and insulin-like growth factor II expression in the postnatal rat corpus callosum. Insulin-like growth factor I and insulin-like growth factor II mRNA expression increased at 3 days as compared with 1 day whereas the protein expression increased up to 7 days. Insulin-like growth factor I and insulin-like growth factor II immunoexpression was specifically localized in round cells confirmed by double immunofluorescence with OX-42 to be the amoeboid microglial cells. Insulin-like growth factor I expression was observed up to 7 days in amoeboid microglial cells while insulin-like growth factor II expression was detected in 1-3 day old rats. Exposure of primary rat microglial cell cultures to lipopolysaccharide increased insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression significantly along with their immunoexpression in microglial cells. The lipopolysaccharide-induced increase in insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression was significantly decreased with all-trans-retinoic acid. We conclude that insulin-like growth factor I and insulin-like growth factor II expression in amoeboid microglial cells in the developing brain is related to their activation. Once the activation is inhibited, either by transformation of the amoeboid microglial cells into ramified microglia regarded as resting cells or as shown by the effect of all-trans-retinoic acid administration, insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression is downregulated.

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  • Authors+Show Affiliations

    ,

    Department of Anatomy, Yong Loo Lin School of Medicine, Blk MD10, 4 Medical Drive, National University of Singapore, Singapore 117597. antkaurc@nus.edu.sg

    , ,

    Source

    Neuroscience 138:4 2006 pg 1233-44

    MeSH

    Animals
    Animals, Newborn
    Brain
    CD11b Antigen
    Cell Differentiation
    Cell Shape
    Cells, Cultured
    Chemotaxis
    Corpus Callosum
    Down-Regulation
    Gene Expression Regulation, Developmental
    Glial Fibrillary Acidic Protein
    Gliosis
    Insulin-Like Growth Factor I
    Insulin-Like Growth Factor II
    Lipopolysaccharides
    Microglia
    Neural Pathways
    RNA, Messenger
    Rats
    Rats, Wistar
    Transcriptional Activation
    Tretinoin
    Up-Regulation

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    16448778

    Citation

    Kaur, C, et al. "Insulin-like Growth Factor I and II Expression and Modulation in Amoeboid Microglial Cells By Lipopolysaccharide and Retinoic Acid." Neuroscience, vol. 138, no. 4, 2006, pp. 1233-44.
    Kaur C, Sivakumar V, Dheen ST, et al. Insulin-like growth factor I and II expression and modulation in amoeboid microglial cells by lipopolysaccharide and retinoic acid. Neuroscience. 2006;138(4):1233-44.
    Kaur, C., Sivakumar, V., Dheen, S. T., & Ling, E. A. (2006). Insulin-like growth factor I and II expression and modulation in amoeboid microglial cells by lipopolysaccharide and retinoic acid. Neuroscience, 138(4), pp. 1233-44.
    Kaur C, et al. Insulin-like Growth Factor I and II Expression and Modulation in Amoeboid Microglial Cells By Lipopolysaccharide and Retinoic Acid. Neuroscience. 2006;138(4):1233-44. PubMed PMID: 16448778.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Insulin-like growth factor I and II expression and modulation in amoeboid microglial cells by lipopolysaccharide and retinoic acid. AU - Kaur,C, AU - Sivakumar,V, AU - Dheen,S T, AU - Ling,E A, Y1 - 2006/01/31/ PY - 2005/08/25/received PY - 2005/11/23/revised PY - 2005/12/01/accepted PY - 2006/2/2/pubmed PY - 2006/6/9/medline PY - 2006/2/2/entrez SP - 1233 EP - 44 JF - Neuroscience JO - Neuroscience VL - 138 IS - 4 N2 - Insulin-like growth factors I and II are known to regulate the development of the CNS. We examined the developmental changes in insulin-like growth factor I and insulin-like growth factor II expression in the postnatal rat corpus callosum. Insulin-like growth factor I and insulin-like growth factor II mRNA expression increased at 3 days as compared with 1 day whereas the protein expression increased up to 7 days. Insulin-like growth factor I and insulin-like growth factor II immunoexpression was specifically localized in round cells confirmed by double immunofluorescence with OX-42 to be the amoeboid microglial cells. Insulin-like growth factor I expression was observed up to 7 days in amoeboid microglial cells while insulin-like growth factor II expression was detected in 1-3 day old rats. Exposure of primary rat microglial cell cultures to lipopolysaccharide increased insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression significantly along with their immunoexpression in microglial cells. The lipopolysaccharide-induced increase in insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression was significantly decreased with all-trans-retinoic acid. We conclude that insulin-like growth factor I and insulin-like growth factor II expression in amoeboid microglial cells in the developing brain is related to their activation. Once the activation is inhibited, either by transformation of the amoeboid microglial cells into ramified microglia regarded as resting cells or as shown by the effect of all-trans-retinoic acid administration, insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression is downregulated. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/16448778/Insulin_like_growth_factor_I_and_II_expression_and_modulation_in_amoeboid_microglial_cells_by_lipopolysaccharide_and_retinoic_acid_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(05)01390-4 DB - PRIME DP - Unbound Medicine ER -