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Insulin-like growth factor I and II expression and modulation in amoeboid microglial cells by lipopolysaccharide and retinoic acid.
Neuroscience 2006; 138(4):1233-44N

Abstract

Insulin-like growth factors I and II are known to regulate the development of the CNS. We examined the developmental changes in insulin-like growth factor I and insulin-like growth factor II expression in the postnatal rat corpus callosum. Insulin-like growth factor I and insulin-like growth factor II mRNA expression increased at 3 days as compared with 1 day whereas the protein expression increased up to 7 days. Insulin-like growth factor I and insulin-like growth factor II immunoexpression was specifically localized in round cells confirmed by double immunofluorescence with OX-42 to be the amoeboid microglial cells. Insulin-like growth factor I expression was observed up to 7 days in amoeboid microglial cells while insulin-like growth factor II expression was detected in 1-3 day old rats. Exposure of primary rat microglial cell cultures to lipopolysaccharide increased insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression significantly along with their immunoexpression in microglial cells. The lipopolysaccharide-induced increase in insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression was significantly decreased with all-trans-retinoic acid. We conclude that insulin-like growth factor I and insulin-like growth factor II expression in amoeboid microglial cells in the developing brain is related to their activation. Once the activation is inhibited, either by transformation of the amoeboid microglial cells into ramified microglia regarded as resting cells or as shown by the effect of all-trans-retinoic acid administration, insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression is downregulated.

Authors+Show Affiliations

Department of Anatomy, Yong Loo Lin School of Medicine, Blk MD10, 4 Medical Drive, National University of Singapore, Singapore 117597. antkaurc@nus.edu.sgNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16448778

Citation

Kaur, C, et al. "Insulin-like Growth Factor I and II Expression and Modulation in Amoeboid Microglial Cells By Lipopolysaccharide and Retinoic Acid." Neuroscience, vol. 138, no. 4, 2006, pp. 1233-44.
Kaur C, Sivakumar V, Dheen ST, et al. Insulin-like growth factor I and II expression and modulation in amoeboid microglial cells by lipopolysaccharide and retinoic acid. Neuroscience. 2006;138(4):1233-44.
Kaur, C., Sivakumar, V., Dheen, S. T., & Ling, E. A. (2006). Insulin-like growth factor I and II expression and modulation in amoeboid microglial cells by lipopolysaccharide and retinoic acid. Neuroscience, 138(4), pp. 1233-44.
Kaur C, et al. Insulin-like Growth Factor I and II Expression and Modulation in Amoeboid Microglial Cells By Lipopolysaccharide and Retinoic Acid. Neuroscience. 2006;138(4):1233-44. PubMed PMID: 16448778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Insulin-like growth factor I and II expression and modulation in amoeboid microglial cells by lipopolysaccharide and retinoic acid. AU - Kaur,C, AU - Sivakumar,V, AU - Dheen,S T, AU - Ling,E A, Y1 - 2006/01/31/ PY - 2005/08/25/received PY - 2005/11/23/revised PY - 2005/12/01/accepted PY - 2006/2/2/pubmed PY - 2006/6/9/medline PY - 2006/2/2/entrez SP - 1233 EP - 44 JF - Neuroscience JO - Neuroscience VL - 138 IS - 4 N2 - Insulin-like growth factors I and II are known to regulate the development of the CNS. We examined the developmental changes in insulin-like growth factor I and insulin-like growth factor II expression in the postnatal rat corpus callosum. Insulin-like growth factor I and insulin-like growth factor II mRNA expression increased at 3 days as compared with 1 day whereas the protein expression increased up to 7 days. Insulin-like growth factor I and insulin-like growth factor II immunoexpression was specifically localized in round cells confirmed by double immunofluorescence with OX-42 to be the amoeboid microglial cells. Insulin-like growth factor I expression was observed up to 7 days in amoeboid microglial cells while insulin-like growth factor II expression was detected in 1-3 day old rats. Exposure of primary rat microglial cell cultures to lipopolysaccharide increased insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression significantly along with their immunoexpression in microglial cells. The lipopolysaccharide-induced increase in insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression was significantly decreased with all-trans-retinoic acid. We conclude that insulin-like growth factor I and insulin-like growth factor II expression in amoeboid microglial cells in the developing brain is related to their activation. Once the activation is inhibited, either by transformation of the amoeboid microglial cells into ramified microglia regarded as resting cells or as shown by the effect of all-trans-retinoic acid administration, insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression is downregulated. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/16448778/Insulin_like_growth_factor_I_and_II_expression_and_modulation_in_amoeboid_microglial_cells_by_lipopolysaccharide_and_retinoic_acid_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(05)01390-4 DB - PRIME DP - Unbound Medicine ER -