Tags

Type your tag names separated by a space and hit enter

Expression analysis of barley (Hordeum vulgare L.) during salinity stress.
Funct Integr Genomics. 2006 Apr; 6(2):143-56.FI

Abstract

Barley (Hordeum vulgare L.) is a salt-tolerant crop species with considerable economic importance in salinity-affected arid and semiarid regions of the world. In this work, barley cultivar Morex was used for transcriptional profiling during salinity stress using a microarray containing approximately 22,750 probe sets. The experiment was designed to target the early responses of genes to a salinity stress at seedling stage. We found a comparable number of probe sets up-regulated and down-regulated in response to salinity. The differentially expressed genes were broadly characterized using gene ontology and through expression-based hierarchical clustering to identify interesting features in the data. A prominent feature of the response to salinity was the induction of genes involved in jasmonic acid biosynthesis and genes known to respond to jasmonic acid treatment. A large number of abiotic stress (heat, drought, and low temperature) related genes were also found to be responsive to salinity stress. Our results also indicate osmoprotection to be an early response of barley under salinity stress. Additionally, we compared the results of our studies with two other reports characterizing gene expression of barley under salinity stress and found very few genes in common.

Authors+Show Affiliations

Department of Botany and Plant Sciences, University of California, Riverside, CA, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16450154

Citation

Walia, Harkamal, et al. "Expression Analysis of Barley (Hordeum Vulgare L.) During Salinity Stress." Functional & Integrative Genomics, vol. 6, no. 2, 2006, pp. 143-56.
Walia H, Wilson C, Wahid A, et al. Expression analysis of barley (Hordeum vulgare L.) during salinity stress. Funct Integr Genomics. 2006;6(2):143-56.
Walia, H., Wilson, C., Wahid, A., Condamine, P., Cui, X., & Close, T. J. (2006). Expression analysis of barley (Hordeum vulgare L.) during salinity stress. Functional & Integrative Genomics, 6(2), 143-56.
Walia H, et al. Expression Analysis of Barley (Hordeum Vulgare L.) During Salinity Stress. Funct Integr Genomics. 2006;6(2):143-56. PubMed PMID: 16450154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression analysis of barley (Hordeum vulgare L.) during salinity stress. AU - Walia,Harkamal, AU - Wilson,Clyde, AU - Wahid,Abdul, AU - Condamine,Pascal, AU - Cui,Xinping, AU - Close,Timothy J, Y1 - 2006/02/01/ PY - 2005/08/10/received PY - 2005/09/25/accepted PY - 2005/09/25/revised PY - 2006/2/2/pubmed PY - 2009/5/1/medline PY - 2006/2/2/entrez SP - 143 EP - 56 JF - Functional & integrative genomics JO - Funct Integr Genomics VL - 6 IS - 2 N2 - Barley (Hordeum vulgare L.) is a salt-tolerant crop species with considerable economic importance in salinity-affected arid and semiarid regions of the world. In this work, barley cultivar Morex was used for transcriptional profiling during salinity stress using a microarray containing approximately 22,750 probe sets. The experiment was designed to target the early responses of genes to a salinity stress at seedling stage. We found a comparable number of probe sets up-regulated and down-regulated in response to salinity. The differentially expressed genes were broadly characterized using gene ontology and through expression-based hierarchical clustering to identify interesting features in the data. A prominent feature of the response to salinity was the induction of genes involved in jasmonic acid biosynthesis and genes known to respond to jasmonic acid treatment. A large number of abiotic stress (heat, drought, and low temperature) related genes were also found to be responsive to salinity stress. Our results also indicate osmoprotection to be an early response of barley under salinity stress. Additionally, we compared the results of our studies with two other reports characterizing gene expression of barley under salinity stress and found very few genes in common. SN - 1438-793X UR - https://www.unboundmedicine.com/medline/citation/16450154/Expression_analysis_of_barley__Hordeum_vulgare_L___during_salinity_stress_ DB - PRIME DP - Unbound Medicine ER -