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Calcitonin gene-related peptide partially mediates nociception in acute experimental pancreatitis.
Surgery. 2006 Feb; 139(2):197-201.S

Abstract

BACKGROUND

The mechanism by which pancreatitis causes pain is unknown. The neuropeptide calcitonin gene-related peptide (CGRP) is released after sensory nerve activation and promotes nociceptive signaling in models of visceral pain. We hypothesized that acute pancreatitis leads to the activation of pancreatic sensory neurons that release CGRP in the dorsal horn of the spinal cord. This signal is ultimately transmitted to the brain, and pain is sensed.

METHODS

To induce pancreatitis, rats were injected with l-arginine (500 mg/kg) intraperitoneally or saline (control). Pancreatitis was confirmed by measuring serum amylase and evaluating pancreatic histology. Activation of nociceptive pathways was evaluated by counting Fos-like immunoreactive nuclei (FLI) in the dorsal horn of the spinal cord at T3-L1. Some animals received the CGRP antagonist CGRP(8-37) (50 microg intrathecally) 2 hours before perfusion. Animals were compared using a 2-tailed t test.

RESULTS

l-Arginine treatment induced acute necrotizing pancreatitis in the rat at 24 hours. l-Arginine (24 hours) increased FLI in the dorsal horn of the spinal cord, with a peak effect at L1. Intrathecal administration of CGRP(8-37) significantly decreased the number of FLI nuclei in the dorsal horn of the spinal cord in T11-L1.

CONCLUSIONS

Nociception in the l-arginine model of acute pancreatitis is partially mediated by the release of CGRP in the dorsal horn of the spinal cord. Antagonism of CGRP or its receptors may be useful in treating pain from acute pancreatitis.

Authors+Show Affiliations

Department of Surgery, University of California, San Francisco, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16455328

Citation

Wick, Elizabeth C., et al. "Calcitonin Gene-related Peptide Partially Mediates Nociception in Acute Experimental Pancreatitis." Surgery, vol. 139, no. 2, 2006, pp. 197-201.
Wick EC, Pikios S, Grady EF, et al. Calcitonin gene-related peptide partially mediates nociception in acute experimental pancreatitis. Surgery. 2006;139(2):197-201.
Wick, E. C., Pikios, S., Grady, E. F., & Kirkwood, K. S. (2006). Calcitonin gene-related peptide partially mediates nociception in acute experimental pancreatitis. Surgery, 139(2), 197-201.
Wick EC, et al. Calcitonin Gene-related Peptide Partially Mediates Nociception in Acute Experimental Pancreatitis. Surgery. 2006;139(2):197-201. PubMed PMID: 16455328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Calcitonin gene-related peptide partially mediates nociception in acute experimental pancreatitis. AU - Wick,Elizabeth C, AU - Pikios,Stella, AU - Grady,Eileen F, AU - Kirkwood,Kimberly S, PY - 2005/01/22/received PY - 2005/08/04/revised PY - 2005/08/05/accepted PY - 2006/2/4/pubmed PY - 2006/3/1/medline PY - 2006/2/4/entrez SP - 197 EP - 201 JF - Surgery JO - Surgery VL - 139 IS - 2 N2 - BACKGROUND: The mechanism by which pancreatitis causes pain is unknown. The neuropeptide calcitonin gene-related peptide (CGRP) is released after sensory nerve activation and promotes nociceptive signaling in models of visceral pain. We hypothesized that acute pancreatitis leads to the activation of pancreatic sensory neurons that release CGRP in the dorsal horn of the spinal cord. This signal is ultimately transmitted to the brain, and pain is sensed. METHODS: To induce pancreatitis, rats were injected with l-arginine (500 mg/kg) intraperitoneally or saline (control). Pancreatitis was confirmed by measuring serum amylase and evaluating pancreatic histology. Activation of nociceptive pathways was evaluated by counting Fos-like immunoreactive nuclei (FLI) in the dorsal horn of the spinal cord at T3-L1. Some animals received the CGRP antagonist CGRP(8-37) (50 microg intrathecally) 2 hours before perfusion. Animals were compared using a 2-tailed t test. RESULTS: l-Arginine treatment induced acute necrotizing pancreatitis in the rat at 24 hours. l-Arginine (24 hours) increased FLI in the dorsal horn of the spinal cord, with a peak effect at L1. Intrathecal administration of CGRP(8-37) significantly decreased the number of FLI nuclei in the dorsal horn of the spinal cord in T11-L1. CONCLUSIONS: Nociception in the l-arginine model of acute pancreatitis is partially mediated by the release of CGRP in the dorsal horn of the spinal cord. Antagonism of CGRP or its receptors may be useful in treating pain from acute pancreatitis. SN - 0039-6060 UR - https://www.unboundmedicine.com/medline/citation/16455328/Calcitonin_gene_related_peptide_partially_mediates_nociception_in_acute_experimental_pancreatitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-6060(05)00519-2 DB - PRIME DP - Unbound Medicine ER -