2,5-Dihydroxyacetophenone: a matrix for highly sensitive matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of proteins using manual and automated preparation techniques.Rapid Commun Mass Spectrom. 2006; 20(5):785-9.RC
Abstract
2,5-Dihydroxyacetophenone (DHAP) is presented as a matrix which enables highly sensitive matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometric analysis of peptides, proteins and glycoproteins on AnchorChip targets. Depending on the protein, lower fmol amounts can be detected due to the increased homogeneity and concentration of the crystallization of the analyte/matrix mixture on the anchors. Best results could be generated in the mass range of 8-100 kDa. All sample/matrix preparation steps starting from mixing of DHAP matrix solution with sample solution to the transfer of the mixture to the MALDI-TOF target can be performed manually or automatically allowing low- and high-throughput analyses.
MeSH
Pub Type(s)
Journal Article
Research Support, Non-U.S. Gov't
Language
eng
PubMed ID
16456805
Citation
Wenzel, Thomas, et al. "2,5-Dihydroxyacetophenone: a Matrix for Highly Sensitive Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometric Analysis of Proteins Using Manual and Automated Preparation Techniques." Rapid Communications in Mass Spectrometry : RCM, vol. 20, no. 5, 2006, pp. 785-9.
Wenzel T, Sparbier K, Mieruch T, et al. 2,5-Dihydroxyacetophenone: a matrix for highly sensitive matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of proteins using manual and automated preparation techniques. Rapid Commun Mass Spectrom. 2006;20(5):785-9.
Wenzel, T., Sparbier, K., Mieruch, T., & Kostrzewa, M. (2006). 2,5-Dihydroxyacetophenone: a matrix for highly sensitive matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of proteins using manual and automated preparation techniques. Rapid Communications in Mass Spectrometry : RCM, 20(5), 785-9.
Wenzel T, et al. 2,5-Dihydroxyacetophenone: a Matrix for Highly Sensitive Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometric Analysis of Proteins Using Manual and Automated Preparation Techniques. Rapid Commun Mass Spectrom. 2006;20(5):785-9. PubMed PMID: 16456805.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - 2,5-Dihydroxyacetophenone: a matrix for highly sensitive matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of proteins using manual and automated preparation techniques.
AU - Wenzel,Thomas,
AU - Sparbier,Katrin,
AU - Mieruch,Thorsten,
AU - Kostrzewa,Markus,
PY - 2006/2/4/pubmed
PY - 2006/4/7/medline
PY - 2006/2/4/entrez
SP - 785
EP - 9
JF - Rapid communications in mass spectrometry : RCM
JO - Rapid Commun Mass Spectrom
VL - 20
IS - 5
N2 - 2,5-Dihydroxyacetophenone (DHAP) is presented as a matrix which enables highly sensitive matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometric analysis of peptides, proteins and glycoproteins on AnchorChip targets. Depending on the protein, lower fmol amounts can be detected due to the increased homogeneity and concentration of the crystallization of the analyte/matrix mixture on the anchors. Best results could be generated in the mass range of 8-100 kDa. All sample/matrix preparation steps starting from mixing of DHAP matrix solution with sample solution to the transfer of the mixture to the MALDI-TOF target can be performed manually or automatically allowing low- and high-throughput analyses.
SN - 0951-4198
UR - https://www.unboundmedicine.com/medline/citation/16456805/25_Dihydroxyacetophenone:_a_matrix_for_highly_sensitive_matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometric_analysis_of_proteins_using_manual_and_automated_preparation_techniques_
DB - PRIME
DP - Unbound Medicine
ER -
