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Molecular cloning of the major lethal toxins from two kraits (Bungarus flaviceps and Bungarus candidus).
Toxicon. 2006 Mar 15; 47(4):416-24.T

Abstract

The major lethal toxins present in the venoms of the red-headed krait, Bungarus flaviceps, and the Malayan krait, Bungarus candidus, have both been purified. Each consists of two polypeptide chains, A and B, joined by a disulfide bond. In the present study, primary structures of these toxins were determined by Edman degradation and by nucleotide sequencing of the cDNA clones. Amino acid sequencing of the N-terminus and enzymatically digested peptides revealed that the A and B chains were highly homologous to those of beta-bungarotoxins (beta-Bgts) from Bungarus multicinctus, respectively. We isolated cDNA clones encoding the A and B chains from both B. flaviceps and B. candidus venom gland cDNA libraries using probes designed based on the cDNA sequence of beta-Bgt from B. multicinctus. Two isoforms of the A chain and one isoform of the B chain were obtained from B. flaviceps, and one isoform of the A chain and two isoforms of the B chain were obtained from B. candidus. Both of the two A chains from B. flaviceps are made up of 119 amino acids and comprise 15 cysteine residues, while the A chains of beta-Bgt from other Bungarus species including B. candidus comprise 13 cysteine residues. The B chains from both species are composed of 59 amino acid residues and comprise seven cysteines. In conclusion, the lethal toxin from B. flaviceps is considered to be a novel isoform of beta-Bgt, which has a different pattern of cysteine residues from known beta-Bgts.

Authors+Show Affiliations

Institute of Medicinal Chemistry, Hoshi University, Tokyo 142-8501, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16458338

Citation

Yanoshita, Ryohei, et al. "Molecular Cloning of the Major Lethal Toxins From Two Kraits (Bungarus Flaviceps and Bungarus Candidus)." Toxicon : Official Journal of the International Society On Toxinology, vol. 47, no. 4, 2006, pp. 416-24.
Yanoshita R, Ogawa Y, Murayama N, et al. Molecular cloning of the major lethal toxins from two kraits (Bungarus flaviceps and Bungarus candidus). Toxicon. 2006;47(4):416-24.
Yanoshita, R., Ogawa, Y., Murayama, N., Omori-Satoh, T., Saguchi, K., Higuchi, S., Khow, O., Chanhome, L., Samejima, Y., & Sitprija, V. (2006). Molecular cloning of the major lethal toxins from two kraits (Bungarus flaviceps and Bungarus candidus). Toxicon : Official Journal of the International Society On Toxinology, 47(4), 416-24.
Yanoshita R, et al. Molecular Cloning of the Major Lethal Toxins From Two Kraits (Bungarus Flaviceps and Bungarus Candidus). Toxicon. 2006 Mar 15;47(4):416-24. PubMed PMID: 16458338.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning of the major lethal toxins from two kraits (Bungarus flaviceps and Bungarus candidus). AU - Yanoshita,Ryohei, AU - Ogawa,Yuko, AU - Murayama,Nobuhiro, AU - Omori-Satoh,Tamotsu, AU - Saguchi,Ken-Ichi, AU - Higuchi,Shigesada, AU - Khow,Orawan, AU - Chanhome,Lawan, AU - Samejima,Yuji, AU - Sitprija,Visith, Y1 - 2006/02/02/ PY - 2005/08/29/received PY - 2005/11/25/revised PY - 2005/12/06/accepted PY - 2006/2/7/pubmed PY - 2006/4/21/medline PY - 2006/2/7/entrez SP - 416 EP - 24 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 47 IS - 4 N2 - The major lethal toxins present in the venoms of the red-headed krait, Bungarus flaviceps, and the Malayan krait, Bungarus candidus, have both been purified. Each consists of two polypeptide chains, A and B, joined by a disulfide bond. In the present study, primary structures of these toxins were determined by Edman degradation and by nucleotide sequencing of the cDNA clones. Amino acid sequencing of the N-terminus and enzymatically digested peptides revealed that the A and B chains were highly homologous to those of beta-bungarotoxins (beta-Bgts) from Bungarus multicinctus, respectively. We isolated cDNA clones encoding the A and B chains from both B. flaviceps and B. candidus venom gland cDNA libraries using probes designed based on the cDNA sequence of beta-Bgt from B. multicinctus. Two isoforms of the A chain and one isoform of the B chain were obtained from B. flaviceps, and one isoform of the A chain and two isoforms of the B chain were obtained from B. candidus. Both of the two A chains from B. flaviceps are made up of 119 amino acids and comprise 15 cysteine residues, while the A chains of beta-Bgt from other Bungarus species including B. candidus comprise 13 cysteine residues. The B chains from both species are composed of 59 amino acid residues and comprise seven cysteines. In conclusion, the lethal toxin from B. flaviceps is considered to be a novel isoform of beta-Bgt, which has a different pattern of cysteine residues from known beta-Bgts. SN - 0041-0101 UR - https://www.unboundmedicine.com/medline/citation/16458338/Molecular_cloning_of_the_major_lethal_toxins_from_two_kraits__Bungarus_flaviceps_and_Bungarus_candidus__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(05)00423-X DB - PRIME DP - Unbound Medicine ER -