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Müller glial cells express nestin coupled with glial fibrillary acidic protein in experimentally induced glaucoma in the rat retina.
Neuroscience. 2006 May 12; 139(2):723-32.N

Abstract

This study was aimed to investigate reactive changes of Müller glial cells in rats subjected to experimentally induced glaucoma. In the latter, it is well documented that elevated intraocular pressure leads to the loss of ganglion cells as confirmed in this study. The present results have shown that Müller glial cells as well as astrocytes closely associated with the ganglion cells reacted vigorously to increased intraocular pressure as manifested by the induced and upregulated expression of nestin and glial fibrillar acidic protein. A major finding in glaucomatous rats was the induced expression of nestin together with glial fibrillar acidic protein with the rise of the intraocular pressure beginning at 2 h. The marked nestin expression appeared to be most intense at 1 week after operation and was sustained at 3 weeks. Induced nestin expression in Müller glial cells was demonstrated unequivocally in whole-mount preparation of the retina. In the same tissue preparation, nestin expression was also detected in some astrocytes. Western blotting analysis confirmed a marked increase in expression of nestin and glial fibrillar acidic protein. Present results suggest that nestin as well as glial fibrillar acidic protein is a useful biomarker for retina injury. The induced expression of these intermediate filament proteins in Müller glial cells especially at their end-feet and also in some astrocytes adjoining the neuronal injury suggests a potential neuroprotective mechanism in response to acute rise in intraocular pressure resulting in neuronal degeneration.

Authors+Show Affiliations

Department of Anatomy, Yong Loo Lin School of Medicine, Block MD 10, 4 Medical Drive, National University of Singapore, Singapore 117597.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16458441

Citation

Xue, L P., et al. "Müller Glial Cells Express Nestin Coupled With Glial Fibrillary Acidic Protein in Experimentally Induced Glaucoma in the Rat Retina." Neuroscience, vol. 139, no. 2, 2006, pp. 723-32.
Xue LP, Lu J, Cao Q, et al. Müller glial cells express nestin coupled with glial fibrillary acidic protein in experimentally induced glaucoma in the rat retina. Neuroscience. 2006;139(2):723-32.
Xue, L. P., Lu, J., Cao, Q., Hu, S., Ding, P., & Ling, E. A. (2006). Müller glial cells express nestin coupled with glial fibrillary acidic protein in experimentally induced glaucoma in the rat retina. Neuroscience, 139(2), 723-32.
Xue LP, et al. Müller Glial Cells Express Nestin Coupled With Glial Fibrillary Acidic Protein in Experimentally Induced Glaucoma in the Rat Retina. Neuroscience. 2006 May 12;139(2):723-32. PubMed PMID: 16458441.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Müller glial cells express nestin coupled with glial fibrillary acidic protein in experimentally induced glaucoma in the rat retina. AU - Xue,L P, AU - Lu,J, AU - Cao,Q, AU - Hu,S, AU - Ding,P, AU - Ling,E-A, Y1 - 2006/02/03/ PY - 2005/10/26/received PY - 2005/12/19/revised PY - 2005/12/20/accepted PY - 2006/2/7/pubmed PY - 2006/8/1/medline PY - 2006/2/7/entrez SP - 723 EP - 32 JF - Neuroscience JO - Neuroscience VL - 139 IS - 2 N2 - This study was aimed to investigate reactive changes of Müller glial cells in rats subjected to experimentally induced glaucoma. In the latter, it is well documented that elevated intraocular pressure leads to the loss of ganglion cells as confirmed in this study. The present results have shown that Müller glial cells as well as astrocytes closely associated with the ganglion cells reacted vigorously to increased intraocular pressure as manifested by the induced and upregulated expression of nestin and glial fibrillar acidic protein. A major finding in glaucomatous rats was the induced expression of nestin together with glial fibrillar acidic protein with the rise of the intraocular pressure beginning at 2 h. The marked nestin expression appeared to be most intense at 1 week after operation and was sustained at 3 weeks. Induced nestin expression in Müller glial cells was demonstrated unequivocally in whole-mount preparation of the retina. In the same tissue preparation, nestin expression was also detected in some astrocytes. Western blotting analysis confirmed a marked increase in expression of nestin and glial fibrillar acidic protein. Present results suggest that nestin as well as glial fibrillar acidic protein is a useful biomarker for retina injury. The induced expression of these intermediate filament proteins in Müller glial cells especially at their end-feet and also in some astrocytes adjoining the neuronal injury suggests a potential neuroprotective mechanism in response to acute rise in intraocular pressure resulting in neuronal degeneration. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/16458441/Müller_glial_cells_express_nestin_coupled_with_glial_fibrillary_acidic_protein_in_experimentally_induced_glaucoma_in_the_rat_retina_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(05)01496-X DB - PRIME DP - Unbound Medicine ER -