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Eya1 is required for lineage-specific differentiation, but not for cell survival in the zebrafish adenohypophysis.
Dev Biol. 2006 Apr 01; 292(1):189-204.DB

Abstract

The homeodomain transcription factor Six1 and its modulator, the protein phosphatase Eya1, cooperate to promote cell differentiation and survival during mouse organ development. Here, we studied the effects caused by loss of eya1 and six1 function on pituitary development in zebrafish. eya1 and six1 are co-expressed in all adenohypophyseal cells. Nevertheless, eya1 (aal, dog) mutants show lineage-specific defects, defining corticotropes, melanotropes, and gonadotropes as an Eya1-dependent lineage, which is complementary to the Pit1 lineage. Furthermore, eya1 is required for maintenance of pit1 expression, leading to subsequent loss of cognate hormone gene expression in thyrotropes and somatotropes of mutant embryos, whereas prolactin expression in lactotropes persists. In contrast to other organs, adenohypophyseal cells of eya1 mutants do not become apoptotic, and the adenohypophysis remains at rather normal size. Also, cells do not trans-differentiate, as in the case of pit1 mutants, but display morphological features characteristic for nonsecretory cells. Some of the adenohypophyseal defects of eya1 mutants are moderately enhanced in combination with antisense-mediated loss of Six1 function, which per se does not affect pituitary cell differentiation. In conclusion, this is the first report of an essential role of Eya1 during pituitary development in vertebrates. Eya1 is required for lineage-specific differentiation of adenohypophyseal cells, but not for their survival, thereby uncoupling the differentiation-promoting and anti-apoptotic effects of Eya proteins seen in other tissues.

Authors+Show Affiliations

Max-Planck Institute of Immunobiology, Stuebeweg 51, 79108 Freiburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16458879

Citation

Nica, Gabriela, et al. "Eya1 Is Required for Lineage-specific Differentiation, but Not for Cell Survival in the Zebrafish Adenohypophysis." Developmental Biology, vol. 292, no. 1, 2006, pp. 189-204.
Nica G, Herzog W, Sonntag C, et al. Eya1 is required for lineage-specific differentiation, but not for cell survival in the zebrafish adenohypophysis. Dev Biol. 2006;292(1):189-204.
Nica, G., Herzog, W., Sonntag, C., Nowak, M., Schwarz, H., Zapata, A. G., & Hammerschmidt, M. (2006). Eya1 is required for lineage-specific differentiation, but not for cell survival in the zebrafish adenohypophysis. Developmental Biology, 292(1), 189-204.
Nica G, et al. Eya1 Is Required for Lineage-specific Differentiation, but Not for Cell Survival in the Zebrafish Adenohypophysis. Dev Biol. 2006 Apr 1;292(1):189-204. PubMed PMID: 16458879.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Eya1 is required for lineage-specific differentiation, but not for cell survival in the zebrafish adenohypophysis. AU - Nica,Gabriela, AU - Herzog,Wiebke, AU - Sonntag,Carmen, AU - Nowak,Matthias, AU - Schwarz,Heinz, AU - Zapata,Agustin G, AU - Hammerschmidt,Matthias, Y1 - 2006/02/03/ PY - 2005/07/08/received PY - 2005/12/19/revised PY - 2005/12/20/accepted PY - 2006/2/7/pubmed PY - 2006/5/12/medline PY - 2006/2/7/entrez SP - 189 EP - 204 JF - Developmental biology JO - Dev. Biol. VL - 292 IS - 1 N2 - The homeodomain transcription factor Six1 and its modulator, the protein phosphatase Eya1, cooperate to promote cell differentiation and survival during mouse organ development. Here, we studied the effects caused by loss of eya1 and six1 function on pituitary development in zebrafish. eya1 and six1 are co-expressed in all adenohypophyseal cells. Nevertheless, eya1 (aal, dog) mutants show lineage-specific defects, defining corticotropes, melanotropes, and gonadotropes as an Eya1-dependent lineage, which is complementary to the Pit1 lineage. Furthermore, eya1 is required for maintenance of pit1 expression, leading to subsequent loss of cognate hormone gene expression in thyrotropes and somatotropes of mutant embryos, whereas prolactin expression in lactotropes persists. In contrast to other organs, adenohypophyseal cells of eya1 mutants do not become apoptotic, and the adenohypophysis remains at rather normal size. Also, cells do not trans-differentiate, as in the case of pit1 mutants, but display morphological features characteristic for nonsecretory cells. Some of the adenohypophyseal defects of eya1 mutants are moderately enhanced in combination with antisense-mediated loss of Six1 function, which per se does not affect pituitary cell differentiation. In conclusion, this is the first report of an essential role of Eya1 during pituitary development in vertebrates. Eya1 is required for lineage-specific differentiation of adenohypophyseal cells, but not for their survival, thereby uncoupling the differentiation-promoting and anti-apoptotic effects of Eya proteins seen in other tissues. SN - 0012-1606 UR - https://www.unboundmedicine.com/medline/citation/16458879/Eya1_is_required_for_lineage_specific_differentiation_but_not_for_cell_survival_in_the_zebrafish_adenohypophysis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(05)00937-1 DB - PRIME DP - Unbound Medicine ER -