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Enantiomeric separation of synthetic 2,3-dihydroxy-3-phenylpropionate compounds by beta-cyclodextrin-modified capillary electrophoresis.
J Chromatogr A. 2006 Mar 10; 1108(2):258-62.JC

Abstract

A new capillary electrophoretic method was developed for enantiomeric separation and optical impurity analysis of three synthetic 2,3-dihydroxy-3-phenylpropionate compounds using native beta-cyclodextrin (beta-CD) as chiral selector and borate as a background electrolyte. The separation was carried out in uncoated capillary (58.5 cm x 75 microm I.D., effective length 48.5 cm). The results showed that beta-CD as the chiral selector exhibited good enantioselectivity and the baseline separation was obtained at pH 9.8, 200 mM borate buffer containing 1.7% beta-CD at applied voltage 15 kV and capillary temperature 20 degrees C within 15 min. The precision of each tested compound was less than 1.0% at migration time and 5.0% in corrected peak area and the accuracy of the method was in the range of 98.7-105%. Furthermore, the developed method was successfully applied to the determination of the undesirable trace (2S,3R)-(+)-form impurity in the synthetic (2R,3S)-(-)-2,3-dihydroxy-3-phenylpropionate samples.

Authors+Show Affiliations

Zhao Y
Department of Chemistry, Fourth Military Medical University, Xi'an 710032, China.
Yang X
No affiliation info available
Sun X
No affiliation info available
Jiang R
No affiliation info available
Zhang S
No affiliation info available

MeSH

BuffersElectrophoresis, CapillaryHydrogen-Ion ConcentrationPhenylpropionatesReproducibility of ResultsSensitivity and SpecificityStereoisomerismbeta-Cyclodextrins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16458902

Citation

Zhao, Yan, et al. "Enantiomeric Separation of Synthetic 2,3-dihydroxy-3-phenylpropionate Compounds By Beta-cyclodextrin-modified Capillary Electrophoresis." Journal of Chromatography. A, vol. 1108, no. 2, 2006, pp. 258-62.
Zhao Y, Yang X, Sun X, et al. Enantiomeric separation of synthetic 2,3-dihydroxy-3-phenylpropionate compounds by beta-cyclodextrin-modified capillary electrophoresis. J Chromatogr A. 2006;1108(2):258-62.
Zhao, Y., Yang, X., Sun, X., Jiang, R., & Zhang, S. (2006). Enantiomeric separation of synthetic 2,3-dihydroxy-3-phenylpropionate compounds by beta-cyclodextrin-modified capillary electrophoresis. Journal of Chromatography. A, 1108(2), 258-62.
Zhao Y, et al. Enantiomeric Separation of Synthetic 2,3-dihydroxy-3-phenylpropionate Compounds By Beta-cyclodextrin-modified Capillary Electrophoresis. J Chromatogr A. 2006 Mar 10;1108(2):258-62. PubMed PMID: 16458902.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enantiomeric separation of synthetic 2,3-dihydroxy-3-phenylpropionate compounds by beta-cyclodextrin-modified capillary electrophoresis. AU - Zhao,Yan, AU - Yang,Xingbin, AU - Sun,Xiaoli, AU - Jiang,Ru, AU - Zhang,Shengyong, Y1 - 2006/02/03/ PY - 2005/10/22/received PY - 2005/12/30/revised PY - 2006/01/09/accepted PY - 2006/2/7/pubmed PY - 2006/6/22/medline PY - 2006/2/7/entrez SP - 258 EP - 62 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1108 IS - 2 N2 - A new capillary electrophoretic method was developed for enantiomeric separation and optical impurity analysis of three synthetic 2,3-dihydroxy-3-phenylpropionate compounds using native beta-cyclodextrin (beta-CD) as chiral selector and borate as a background electrolyte. The separation was carried out in uncoated capillary (58.5 cm x 75 microm I.D., effective length 48.5 cm). The results showed that beta-CD as the chiral selector exhibited good enantioselectivity and the baseline separation was obtained at pH 9.8, 200 mM borate buffer containing 1.7% beta-CD at applied voltage 15 kV and capillary temperature 20 degrees C within 15 min. The precision of each tested compound was less than 1.0% at migration time and 5.0% in corrected peak area and the accuracy of the method was in the range of 98.7-105%. Furthermore, the developed method was successfully applied to the determination of the undesirable trace (2S,3R)-(+)-form impurity in the synthetic (2R,3S)-(-)-2,3-dihydroxy-3-phenylpropionate samples. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16458902/Enantiomeric_separation_of_synthetic_23_dihydroxy_3_phenylpropionate_compounds_by_beta_cyclodextrin_modified_capillary_electrophoresis_ DB - PRIME DP - Unbound Medicine ER -

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