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Evaluation of fusion between liposomes and erythrocytes for intracellular derivatization of amino acids in cells.
J Chromatogr A. 2006 Mar 31; 1110(1-2):81-5.JC

Abstract

Erythrocytes were fused with liposome for intracellular derivatization of amino acids in cells. The fusion efficiency was evaluated with capillary electrophoresis (CE) and laser-induced fluorescence (LIF) detection. Reagent fluorescein isothiocyanate (FITC) was enveloped in liposomes and introduced into erythrocytes by fusion between liposomes and erythrocytes. The amino acids in the fused cells were derivated by the introduced FITC and the derivated amino acids were extracted for detection by capillary electrophoresis equipped with laser-induced fluorescence detector. The fusion conditions were investigated. It was found that incubation of liposome and erythrocytes in the presence of 13% polyethylene glycol 6000 (PEG 6000) for 15min produced the highest fusion efficiency and kept the erythrocytes stability.

Authors+Show Affiliations

State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

16460741

Citation

Zhang, Hua, et al. "Evaluation of Fusion Between Liposomes and Erythrocytes for Intracellular Derivatization of Amino Acids in Cells." Journal of Chromatography. A, vol. 1110, no. 1-2, 2006, pp. 81-5.
Zhang H, Du J, Zhao J, et al. Evaluation of fusion between liposomes and erythrocytes for intracellular derivatization of amino acids in cells. J Chromatogr A. 2006;1110(1-2):81-5.
Zhang, H., Du, J., Zhao, J., & Li, Y. Z. (2006). Evaluation of fusion between liposomes and erythrocytes for intracellular derivatization of amino acids in cells. Journal of Chromatography. A, 1110(1-2), 81-5.
Zhang H, et al. Evaluation of Fusion Between Liposomes and Erythrocytes for Intracellular Derivatization of Amino Acids in Cells. J Chromatogr A. 2006 Mar 31;1110(1-2):81-5. PubMed PMID: 16460741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of fusion between liposomes and erythrocytes for intracellular derivatization of amino acids in cells. AU - Zhang,Hua, AU - Du,Juan, AU - Zhao,Jie, AU - Li,Yue-zhong, Y1 - 2006/02/07/ PY - 2005/10/12/received PY - 2006/01/02/revised PY - 2006/01/18/accepted PY - 2006/2/8/pubmed PY - 2006/6/29/medline PY - 2006/2/8/entrez SP - 81 EP - 5 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1110 IS - 1-2 N2 - Erythrocytes were fused with liposome for intracellular derivatization of amino acids in cells. The fusion efficiency was evaluated with capillary electrophoresis (CE) and laser-induced fluorescence (LIF) detection. Reagent fluorescein isothiocyanate (FITC) was enveloped in liposomes and introduced into erythrocytes by fusion between liposomes and erythrocytes. The amino acids in the fused cells were derivated by the introduced FITC and the derivated amino acids were extracted for detection by capillary electrophoresis equipped with laser-induced fluorescence detector. The fusion conditions were investigated. It was found that incubation of liposome and erythrocytes in the presence of 13% polyethylene glycol 6000 (PEG 6000) for 15min produced the highest fusion efficiency and kept the erythrocytes stability. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16460741/Evaluation_of_fusion_between_liposomes_and_erythrocytes_for_intracellular_derivatization_of_amino_acids_in_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(06)00213-5 DB - PRIME DP - Unbound Medicine ER -