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Strategy for identification of Bacillus cereus and Bacillus thuringiensis strains closely related to Bacillus anthracis.
Appl Environ Microbiol 2006; 72(2):1295-301AE

Abstract

Bacillus cereus strains that are genetically closely related to B. anthracis can display anthrax-like virulence traits (A. R. Hoffmaster et al., Proc. Natl. Acad. Sci. USA 101:8449-8454, 2004). Hence, approaches that rapidly identify these "near neighbors" are of great interest for the study of B. anthracis virulence mechanisms, as well as to prevent the use of such strains for B. anthracis-based bioweapon development. Here, a strategy is proposed for the identification of near neighbors of B. anthracis based on single nucleotide polymorphisms (SNP) in the 16S-23S rRNA intergenic spacer (ITS) containing tRNA genes, characteristic of B. anthracis. By using restriction site insertion-PCR (RSI-PCR) the presence of two SNP typical of B. anthracis was screened in 126 B. cereus group strains of different origin. Two B. cereus strains and one B. thuringiensis strain showed RSI-PCR profiles identical to that of B. anthracis. The sequencing of the entire ITS containing tRNA genes revealed two of the strains to be identical to B. anthracis. The strict relationship with B. anthracis was confirmed by multilocus sequence typing (MLST) of four other independent loci: cerA, plcR, AC-390, and SG-749. The relationship to B. anthracis of the three strains described by MLST was comparable and even higher to that of four B. cereus strains associated with periodontitis in humans and previously reported as the closest known strains to B. anthracis. SNP in ITS containing tRNA genes combined with RSI-PCR provide a very efficient tool for the identification of strains closely related to B. anthracis.

Authors+Show Affiliations

Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli Studi di Milano, via Celoria 2, 20133 Milan, Italy. daniele.daffonchio@unimi.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16461679

Citation

Daffonchio, Daniele, et al. "Strategy for Identification of Bacillus Cereus and Bacillus Thuringiensis Strains Closely Related to Bacillus Anthracis." Applied and Environmental Microbiology, vol. 72, no. 2, 2006, pp. 1295-301.
Daffonchio D, Raddadi N, Merabishvili M, et al. Strategy for identification of Bacillus cereus and Bacillus thuringiensis strains closely related to Bacillus anthracis. Appl Environ Microbiol. 2006;72(2):1295-301.
Daffonchio, D., Raddadi, N., Merabishvili, M., Cherif, A., Carmagnola, L., Brusetti, L., ... Borin, S. (2006). Strategy for identification of Bacillus cereus and Bacillus thuringiensis strains closely related to Bacillus anthracis. Applied and Environmental Microbiology, 72(2), pp. 1295-301.
Daffonchio D, et al. Strategy for Identification of Bacillus Cereus and Bacillus Thuringiensis Strains Closely Related to Bacillus Anthracis. Appl Environ Microbiol. 2006;72(2):1295-301. PubMed PMID: 16461679.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Strategy for identification of Bacillus cereus and Bacillus thuringiensis strains closely related to Bacillus anthracis. AU - Daffonchio,Daniele, AU - Raddadi,Noura, AU - Merabishvili,Maya, AU - Cherif,Ameur, AU - Carmagnola,Lorenzo, AU - Brusetti,Lorenzo, AU - Rizzi,Aurora, AU - Chanishvili,Nina, AU - Visca,Paolo, AU - Sharp,Richard, AU - Borin,Sara, PY - 2006/2/8/pubmed PY - 2006/4/21/medline PY - 2006/2/8/entrez SP - 1295 EP - 301 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 72 IS - 2 N2 - Bacillus cereus strains that are genetically closely related to B. anthracis can display anthrax-like virulence traits (A. R. Hoffmaster et al., Proc. Natl. Acad. Sci. USA 101:8449-8454, 2004). Hence, approaches that rapidly identify these "near neighbors" are of great interest for the study of B. anthracis virulence mechanisms, as well as to prevent the use of such strains for B. anthracis-based bioweapon development. Here, a strategy is proposed for the identification of near neighbors of B. anthracis based on single nucleotide polymorphisms (SNP) in the 16S-23S rRNA intergenic spacer (ITS) containing tRNA genes, characteristic of B. anthracis. By using restriction site insertion-PCR (RSI-PCR) the presence of two SNP typical of B. anthracis was screened in 126 B. cereus group strains of different origin. Two B. cereus strains and one B. thuringiensis strain showed RSI-PCR profiles identical to that of B. anthracis. The sequencing of the entire ITS containing tRNA genes revealed two of the strains to be identical to B. anthracis. The strict relationship with B. anthracis was confirmed by multilocus sequence typing (MLST) of four other independent loci: cerA, plcR, AC-390, and SG-749. The relationship to B. anthracis of the three strains described by MLST was comparable and even higher to that of four B. cereus strains associated with periodontitis in humans and previously reported as the closest known strains to B. anthracis. SNP in ITS containing tRNA genes combined with RSI-PCR provide a very efficient tool for the identification of strains closely related to B. anthracis. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/16461679/Strategy_for_identification_of_Bacillus_cereus_and_Bacillus_thuringiensis_strains_closely_related_to_Bacillus_anthracis_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=16461679 DB - PRIME DP - Unbound Medicine ER -