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Txp40, a ubiquitous insecticidal toxin protein from Xenorhabdus and Photorhabdus bacteria.
Appl Environ Microbiol. 2006 Feb; 72(2):1653-62.AE

Abstract

Xenorhabdus and Photorhabdus are gram-negative bacteria that produce a range of proteins that are toxic to insects. We recently identified a novel 42-kDa protein from Xenorhabdus nematophila that was lethal to the larvae of insects such as Galleria mellonella and Helicoverpa armigera when it was injected at doses of 30 to 40 ng/g larvae. In the present work, the toxin gene txp40 was identified in another 59 strains of Xenorhabdus and Photorhabdus, indicating that it is both highly conserved and widespread among these bacteria. Recombinant toxin protein was shown to be active against a variety of insect species by direct injection into the larvae of the lepidopteran species G. mellonella, H. armigera, and Plodia interpunctella and the dipteran species Lucilia cuprina. The protein exhibited significant cytotoxicity against two dipteran cell lines and two lepidopteran cell lines but not against a mammalian cell line. Histological data from H. armigera larvae into which the toxin was injected suggested that the primary site of action of the toxin is the midgut, although some damage to the fat body was also observed.

Authors+Show Affiliations

CSIRO Entomology, GPO Box 1700, Acton, ACT 2601, Australia. sue.brown@csiro.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16461722

Citation

Brown, S E., et al. "Txp40, a Ubiquitous Insecticidal Toxin Protein From Xenorhabdus and Photorhabdus Bacteria." Applied and Environmental Microbiology, vol. 72, no. 2, 2006, pp. 1653-62.
Brown SE, Cao AT, Dobson P, et al. Txp40, a ubiquitous insecticidal toxin protein from Xenorhabdus and Photorhabdus bacteria. Appl Environ Microbiol. 2006;72(2):1653-62.
Brown, S. E., Cao, A. T., Dobson, P., Hines, E. R., Akhurst, R. J., & East, P. D. (2006). Txp40, a ubiquitous insecticidal toxin protein from Xenorhabdus and Photorhabdus bacteria. Applied and Environmental Microbiology, 72(2), 1653-62.
Brown SE, et al. Txp40, a Ubiquitous Insecticidal Toxin Protein From Xenorhabdus and Photorhabdus Bacteria. Appl Environ Microbiol. 2006;72(2):1653-62. PubMed PMID: 16461722.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Txp40, a ubiquitous insecticidal toxin protein from Xenorhabdus and Photorhabdus bacteria. AU - Brown,S E, AU - Cao,A T, AU - Dobson,P, AU - Hines,E R, AU - Akhurst,R J, AU - East,P D, PY - 2006/2/8/pubmed PY - 2006/4/21/medline PY - 2006/2/8/entrez SP - 1653 EP - 62 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 72 IS - 2 N2 - Xenorhabdus and Photorhabdus are gram-negative bacteria that produce a range of proteins that are toxic to insects. We recently identified a novel 42-kDa protein from Xenorhabdus nematophila that was lethal to the larvae of insects such as Galleria mellonella and Helicoverpa armigera when it was injected at doses of 30 to 40 ng/g larvae. In the present work, the toxin gene txp40 was identified in another 59 strains of Xenorhabdus and Photorhabdus, indicating that it is both highly conserved and widespread among these bacteria. Recombinant toxin protein was shown to be active against a variety of insect species by direct injection into the larvae of the lepidopteran species G. mellonella, H. armigera, and Plodia interpunctella and the dipteran species Lucilia cuprina. The protein exhibited significant cytotoxicity against two dipteran cell lines and two lepidopteran cell lines but not against a mammalian cell line. Histological data from H. armigera larvae into which the toxin was injected suggested that the primary site of action of the toxin is the midgut, although some damage to the fat body was also observed. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/16461722/Txp40_a_ubiquitous_insecticidal_toxin_protein_from_Xenorhabdus_and_Photorhabdus_bacteria_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=16461722 DB - PRIME DP - Unbound Medicine ER -