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Serotypes and mating types of clinical isolates from feline cryptococcosis in Japan.
J Vet Med Sci. 2006 Jan; 68(1):91-4.JV

Abstract

Most isolates of Cryptococcus neoformans (teleomorph: Filobasidiella neoformans) from human patients and from environmental materials in Japan have been identified as serotype A mating type a by the seroagglutination test and mating experiments. A PCR method using the mating type alpha allele-specific primer of the STE12 gene and the serotype- and mating type-specific primers of the STE20 gene for identification of C. neoformans has been developed. Using the PCR method, conserved strains and clinical isolates from feline cryptococcosis were examined for serotype and the mating type. The results showed that all clinical isolates examined were identified as serotype A, MATalpha, indicating that feline cryptococcsis cases in Japan are caused by C. neoformans serotype A, MATalpha, as is the case in humans.

Authors+Show Affiliations

Okabayashi K
Department of Veterinary Biochemistry, College of Bioresource Sciences, Nihon University, Kanagawa 252-8510, Japan.
Kano R
No affiliation info available
Watanabe T
No affiliation info available
Hasegawa A
No affiliation info available

MeSH

AnimalsCat DiseasesCatsCryptococcosisCryptococcus neoformansDNA PrimersGenes, Mating Type, FungalJapanPolymerase Chain ReactionSerotypingSpecies Specificity

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16462126

Citation

Okabayashi, Ken, et al. "Serotypes and Mating Types of Clinical Isolates From Feline Cryptococcosis in Japan." The Journal of Veterinary Medical Science, vol. 68, no. 1, 2006, pp. 91-4.
Okabayashi K, Kano R, Watanabe T, et al. Serotypes and mating types of clinical isolates from feline cryptococcosis in Japan. J Vet Med Sci. 2006;68(1):91-4.
Okabayashi, K., Kano, R., Watanabe, T., & Hasegawa, A. (2006). Serotypes and mating types of clinical isolates from feline cryptococcosis in Japan. The Journal of Veterinary Medical Science, 68(1), 91-4.
Okabayashi K, et al. Serotypes and Mating Types of Clinical Isolates From Feline Cryptococcosis in Japan. J Vet Med Sci. 2006;68(1):91-4. PubMed PMID: 16462126.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serotypes and mating types of clinical isolates from feline cryptococcosis in Japan. AU - Okabayashi,Ken, AU - Kano,Rui, AU - Watanabe,Toshi, AU - Hasegawa,Atsuhiko, PY - 2006/2/8/pubmed PY - 2006/9/23/medline PY - 2006/2/8/entrez SP - 91 EP - 4 JF - The Journal of veterinary medical science JO - J Vet Med Sci VL - 68 IS - 1 N2 - Most isolates of Cryptococcus neoformans (teleomorph: Filobasidiella neoformans) from human patients and from environmental materials in Japan have been identified as serotype A mating type a by the seroagglutination test and mating experiments. A PCR method using the mating type alpha allele-specific primer of the STE12 gene and the serotype- and mating type-specific primers of the STE20 gene for identification of C. neoformans has been developed. Using the PCR method, conserved strains and clinical isolates from feline cryptococcosis were examined for serotype and the mating type. The results showed that all clinical isolates examined were identified as serotype A, MATalpha, indicating that feline cryptococcsis cases in Japan are caused by C. neoformans serotype A, MATalpha, as is the case in humans. SN - 0916-7250 UR - https://www.unboundmedicine.com/medline/citation/16462126/Serotypes_and_mating_types_of_clinical_isolates_from_feline_cryptococcosis_in_Japan_ DB - PRIME DP - Unbound Medicine ER -